Restriction map for
pF1KSDA0832
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AhdI Alw44I AlwNI ApaI
ApaLI AscI AseI AsnI AspEI
AspHI AvaI AviII AvrII BamHI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BlnI BlpI
BmyI Bpu1102I BsaBI BseAI BseRI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
CelII ClaI CpoI DraII DsaI
EagI Eam1105I EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HpaI
KasI KspI MamI MfeI MluI
MroI MslI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 410 GGTACC G^GTACC
AccI 940 GTATAC GT^[AC][GT]AC
AcyI 187 GACGCC G[GA]^CG[CT]C
AgeI 944 ACCGGT A^CCGGT
AocI 1269 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1191 AGCGCT AGC^GCT
Asp718I 410 GGTACC G^GTACC
AspI 151 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 885 GGACC G^G[AT]CC
BanI 410 GGTACC G^G[CT][GA]CC
BbvCI 670 CCTCAGC CCTCAGC(-5/-2)
BfaI 1318 CTAG C^TAG
BglII 743 AGATCT A^GATCT
BpmI 312 CTCCAG CTGGAG(16/14)
BsaAI 608 TACGTG [CT]AC^GT[GA]
BsaHI 187 GACGCC G[GA]^CG[CT]C
BsaI 947 GGTCTC GGTCTC(1/5)
BsiEI 816 CGACCG CG[GA][CT]^CG
BsmI 448 GCATTC GAATGC(1/-1)
BspHI 554 TCATGA T^CATGA
BsrFI 944 ACCGGT [GA]^CCGG[CT]
Bst1107I 940 GTATAC GTA^TAC
BstXI 165 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 940 GTATAC GTA^TAC
Bsu36I 1269 CCTGAGG CC^T[ACGT]AGG
Cfr10I 944 ACCGGT [GA]^CCGG[CT]
DraI 564 TTTAAA TTT^AAA
DraIII 800 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 760 GACCCTACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1191 AGCGCT AGC^GCT
Eco81I 1269 CCTGAGG CC^T[ACGT]AGG
HaeII 1191 AGCGCT [GA]GCGC^[CT]
Hin1I 187 GACGCC G[GA]^CG[CT]C
HincII 157 GTCAAC GT[CT]^[GA]AC
HindII 157 GTCAAC GT[CT]^[GA]AC
HindIII 1168 AAGCTT A^AGCTT
KpnI 410 GGTACC GGTAC^C
MaeI 1318 CTAG C^TAG
MseI 565 TTAA T^TAA
PflFI 151 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1215 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 944 ACCGGT A^CCGGT
PshAI 1228 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 554 TCATGA T^CATGA
SphI 575 GCATGC GCATG^C
StuI 1020 AGGCCT AGG^CCT
Tru9I 565 TTAA T^TAA
Tth111I 151 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1215 CCAGCACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 920 AAATTT [GA]^AATT[CT]
1093 GAATTT
AlwI 331 GATCC GGATC(4/5)
927 GATCC
ApoI 920 AAATTT [GA]^AATT[CT]
1093 GAATTT
Asp700I 52 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1156 GAAGCCGTTC
BalI 219 TGGCCA TGG^CCA
1212 TGGCCA
BbsI 1000 GAAGAC GAAGAC(2/6)
1225 GAAGAC
BglI 170 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
439 GCCTGCAAGGC
BpuAI 1000 GAAGAC GAAGAC(2/6)
1225 GAAGAC
BsaWI 735 ACCGGA [AT]^CCGG[AT]
944 ACCGGT
BsgI 40 CTGCAC GTGCAG(16/14)
1294 GTGCAG
BsrI 191 CCAGT ACTGG(1/-1)
1005 CCAGT
BstYI 330 AGATCC [GA]^GATC[CT]
743 AGATCT
Cfr13I 283 GGGCC G^G[ACGT]CC
885 GGACC
DdeI 671 CTCAG C^T[ACGT]AG
1270 CTGAG
EarI 180 CTCTTC CTCTTC(1/4)
52 GAAGAG
HapII 736 CCGG C^CGG
945 CCGG
HpaII 736 CCGG C^CGG
945 CCGG
HphI 394 GGTGA GGTGA(8/7)
1100 TCACC
Ksp632I 180 CTCTTC CTCTTC(1/4)
52 GAAGAG
MaeII 528 ACGT A^CGT
609 ACGT
MflI 330 AGATCC [GA]^GATC[CT]
743 AGATCT
MluNI 219 TGGCCA TGG^CCA
1212 TGGCCA
MscI 219 TGGCCA TGG^CCA
1212 TGGCCA
MspI 736 CCGG C^CGG
945 CCGG
NlaIV 123 GGAACC GG[ACGT]^[ACGT]CC
410 GGTACC
NspI 353 ACATGC [GA]CATG^[CT]
575 GCATGC
PleI 238 GACTC GAGTC(4/5)
1132 GACTC
SapI 52 GAAGAGC GCTCTTC(1/4)
179 GCTCTTC
Sau96I 283 GGGCC G^G[ACGT]CC
885 GGACC
XhoII 330 AGATCC [GA]^GATC[CT]
743 AGATCT
XmnI 52 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1156 GAAGCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI BbvI BsaJI
BsiYI BslI BsmAI BsmFI BssKI
BstF5I BstNI Cac8I CfoI DpnII
EaeI EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HinfI
ItaI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
PstI PvuII RsaI Sau3AI ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI