Restriction map for
pF1KSDA0831
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflIII AgeI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspI AvaII AviII BalI BbeI
BbrPI BbvCI BciVI BclI BglI
BglII BlpI Bpu1102I BsaAI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 1041 GGCGTC G[GA]^CG[CT]C
AflII 207 CTTAAG C^TTAAG
AhdI 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 783 CCTCAGG CC^T[ACGT]AGG
ApaI 838 GGGCCC GGGCC^C
Asp700I 76 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 695 CTCGAG C^[CT]CG[GA]G
AvrII 825 CCTAGG C^CTAGG
BamHI 885 GGATCC G^GATCC
BanI 131 GGCACC G^G[CT][GA]CC
BbsI 324 GTCTTC GAAGAC(2/6)
BcgI 1090 GCAGCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 207 CTTAAG C^TTAAG
BlnI 825 CCTAGG C^CTAGG
BpuAI 324 GTCTTC GAAGAC(2/6)
BsaBI 625 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1041 GGCGTC G[GA]^CG[CT]C
BsaI 308 GAGACC GGTCTC(1/5)
BsgI 1067 GTGCAG GTGCAG(16/14)
BsmI 714 GAATGC GAATGC(1/-1)
BsoBI 695 CTCGAG C^[CT]CG[GA]G
BsrBI 915 GAGCGG CCGCTC(-3/-3)
BstF5I 1076 GGATG GGATG(2/0)
BstYI 885 GGATCC [GA]^GATC[CT]
Bsu36I 783 CCTCAGG CC^T[ACGT]AGG
DraI 1114 TTTAAA TTT^AAA
DsaI 872 CCATGG C^C[GA][CT]GG
Eam1105I 404 GACGATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 783 CCTCAGG CC^T[ACGT]AGG
EcoRV 994 GATATC GAT^ATC
FauI 313 CCCGC CCCGC(4/6)
FokI 1076 GGATG GGATG(9/13)
Hin1I 1041 GGCGTC G[GA]^CG[CT]C
HincII 601 GTCAAC GT[CT]^[GA]AC
HindII 601 GTCAAC GT[CT]^[GA]AC
MamI 625 GATGTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 885 GGATCC [GA]^GATC[CT]
MwoI 356 GCACTGTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 872 CCATGG C^CATGG
NruI 1055 TCGCGA TCG^CGA
NspI 747 GCATGT [GA]CATG^[CT]
PaeR7I 695 CTCGAG C^TCGAG
PflMI 1005 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1088 CTGCAG CTGCA^G
SacI 259 GAGCTC GAGCT^C
SspI 721 AATATT AAT^ATT
StuI 78 AGGCCT AGG^CCT
Van91I 1005 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 872 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 695 CTCGAG C^TCGAG
XhoII 885 GGATCC [GA]^GATC[CT]
XmnI 76 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 928 CGCG CG^CG
1056 CGCG
AciI 314 CCGC CCGC(-3/-1)
917 GCGG
AfaI 798 GTAC GT^AC
850 GTAC
BanII 259 GAGCTC G[GA]GC[CT]^C
838 GGGCCC
BfaI 826 CTAG C^TAG
977 CTAG
BpmI 351 CTCCAG CTGGAG(16/14)
899 CTGGAG
BsiYI 472 CCTAACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1005 CCAGTCTGTGG
BslI 472 CCTAACAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1005 CCAGTCTGTGG
BspMI 830 GCAGGT ACCTGC(4/8)
1069 GCAGGT
BstEII 178 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
BstPI 178 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
BstUI 928 CGCG CG^CG
1056 CGCG
CfoI 577 GCGC GCG^C
927 GCGC
EcoO65I 178 GGTGACC G^GT[ACGT]ACC
1101 GGTGACC
HhaI 577 GCGC GCG^C
927 GCGC
HinP1I 577 GCGC G^CGC
927 GCGC
MaeI 826 CTAG C^TAG
977 CTAG
MspA1I 501 CAGCTG C[AC]G^C[GT]G
595 CAGCTG
MvnI 928 CGCG CG^CG
1056 CGCG
PleI 868 GAGTC GAGTC(4/5)
1026 GAGTC
PvuII 501 CAGCTG CAG^CTG
595 CAGCTG
RsaI 798 GTAC GT^AC
850 GTAC
SexAI 182 ACCTGGT A^CC[AT]GGT
800 ACCTGGT
SfcI 498 CTACAG C^T[GA][CT]AG
1088 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AcsI AluI AlwI ApoI AspHI
BbvI BcnI BmyI BsaJI BseRI
BsiHKAI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HapII HgaI HindIII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MspI
MvaI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I