Restriction map for
pF1KSDA0817
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# NO CUT ENZYMES ( 51 enzymes) #
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AclI AflII AgeI Aor51HI AscI
AseI AsnI AviII BciVI BclI
BfrI BsaBI BsiWI BspDI BspHI
BssHII Bst1107I BstBI BstZ17I ClaI
DraI Eco47III EcoRI EcoRV EcoT22I
FbaI FspI HpaI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI SfuI
SgrAI SnaBI SpeI SspI SwaI
XbaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AatII 5576 GACGTC GACGT^C
Acc65I 2750 GGTACC G^GTACC
AccIII 5582 TCCGGA T^CCGGA
AcsI 7246 AAATTC [GA]^AATT[CT]
ApoI 7246 AAATTC [GA]^AATT[CT]
Asp700I 7084 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2750 GGTACC G^GTACC
AspI 766 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 7605 CGAGCACCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 5582 TCCGGA T^CCGGA
BspEI 5582 TCCGGA T^CCGGA
CpoI 3872 CGGACCG CG^G[AT]CCG
FseI 1600 GGCCGGCC GGCCGG^CC
KpnI 2750 GGTACC GGTAC^C
MroI 5582 TCCGGA T^CCGGA
MseI 398 TTAA T^TAA
NdeI 4514 CATATG CA^TATG
PaeR7I 8107 CTCGAG C^TCGAG
PflFI 766 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 811 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 3872 CGGACCG CG^G[AT]CCG
SalI 5573 GTCGAC G^TCGAC
SapI 7707 GCTCTTC GCTCTTC(1/4)
SphI 2584 GCATGC GCATG^C
Tru9I 398 TTAA T^TAA
Tth111I 766 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 8107 CTCGAG C^TCGAG
XmnI 7084 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 4831 GTCTAC GT^[AC][GT]AC
5573 GTCGAC
AvrII 1924 CCTAGG C^CTAGG
3213 CCTAGG
BbrPI 1279 CACGTG CAC^GTG
3147 CACGTG
BbvCI 3084 CCTCAGC CCTCAGC(-5/-2)
5165 CCTCAGC
BglII 1958 AGATCT A^GATCT
2300 AGATCT
BlnI 1924 CCTAGG C^CTAGG
3213 CCTAGG
BsaWI 203 ACCGGA [AT]^CCGG[AT]
5582 TCCGGA
BsiEI 1599 CGGCCG CG[GA][CT]^CG
7215 CGGCCG
BstEII 763 GGTGACC G^GT[ACGT]ACC
7896 GGTCACC
BstPI 763 GGTGACC G^GT[ACGT]ACC
7896 GGTCACC
EagI 1599 CGGCCG C^GGCCG
7215 CGGCCG
EclXI 1599 CGGCCG C^GGCCG
7215 CGGCCG
Eco52I 1599 CGGCCG C^GGCCG
7215 CGGCCG
EcoO65I 763 GGTGACC G^GT[ACGT]ACC
7896 GGTCACC
HincII 5251 GTCAAC GT[CT]^[GA]AC
5573 GTCGAC
HindII 5251 GTCAAC GT[CT]^[GA]AC
5573 GTCGAC
HindIII 335 AAGCTT A^AGCTT
5450 AAGCTT
PmaCI 1279 CACGTG CAC^GTG
3147 CACGTG
PmlI 1279 CACGTG CAC^GTG
3147 CACGTG
SacI 955 GAGCTC GAGCT^C
4909 GAGCTC
SbfI 510 CCTGCAGG CCTGCA^GG
6897 CCTGCAGG
ScaI 6782 AGTACT AGT^ACT
8234 AGTACT
SexAI 5246 ACCAGGT A^CC[AT]GGT
5476 ACCTGGT
SfiI 1839 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
6303 GGCCTCCTGGGCC
SmlI 7618 CTCAAG C^T[CT][GA]AG
8107 CTCGAG
Sse8387I 510 CCTGCAGG CCTGCA^GG
6897 CCTGCAGG
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# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI Eam1105I EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SspBI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI