Restriction map for
pF1KSDA0760
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AccIII AcsI AgeI ApoI AscI
AseI AsnI AvrII BamHI BbeI
BbvCI BcgI BclI BlnI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI DraI
DrdI EcoRI EcoRV EcoT22I FbaI
FseI HpaI KasI KspI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspBI
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 3067 GACGTC GACGT^C
Acc65I 2352 GGTACC G^GTACC
AclI 2617 AACGTT AA^CGTT
AflII 2407 CTTAAG C^TTAAG
AhdI 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2717 AGCGCT AGC^GCT
Asp700I 3618 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2352 GGTACC G^GTACC
AspEI 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2858 TGCGCA TGC^GCA
BbrPI 661 CACGTG CAC^GTG
BciVI 1713 GTATCC GTATCC(6/5)
BfaI 1462 CTAG C^TAG
BfrI 2407 CTTAAG C^TTAAG
BglI 2135 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 661 CACGTG [CT]AC^GT[GA]
BsaBI 3366 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1470 TCCGGT [AT]^CCGG[AT]
BsmI 710 GCATTC GAATGC(1/-1)
BsrDI 2051 GCAATG GCAATG(2/0)
BstEII 2754 GGTGACC G^GT[ACGT]ACC
BstPI 2754 GGTGACC G^GT[ACGT]ACC
EagI 2554 CGGCCG C^GGCCG
Eam1105I 43 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2554 CGGCCG C^GGCCG
Eco47III 2717 AGCGCT AGC^GCT
Eco52I 2554 CGGCCG C^GGCCG
EcoO65I 2754 GGTGACC G^GT[ACGT]ACC
FspI 2858 TGCGCA TGC^GCA
HindIII 3061 AAGCTT A^AGCTT
KpnI 2352 GGTACC GGTAC^C
MaeI 1462 CTAG C^TAG
MamI 3366 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 2459 ACGCGT A^CGCGT
PflFI 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 661 CACGTG CAC^GTG
PmlI 661 CACGTG CAC^GTG
PpuMI 3050 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 2617 AACGTT AA^CGTT
PvuII 682 CAGCTG CAG^CTG
SacI 2920 GAGCTC GAGCT^C
SexAI 3053 ACCTGGT A^CC[AT]GGT
StuI 2261 AGGCCT AGG^CCT
Tth111I 1878 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3618 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 2973 GGCGTC G[GA]^CG[CT]C
3067 GACGTC
AocI 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
BglII 1151 AGATCT A^GATCT
3590 AGATCT
BlpI 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
Bpu1102I 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
BsaHI 2973 GGCGTC G[GA]^CG[CT]C
3067 GACGTC
BsaI 2182 GAGACC GGTCTC(1/5)
3265 GAGACC
BsmBI 134 CGTCTC CGTCTC(1/5)
1320 CGTCTC
BspLU11I 1144 ACATGT A^CATGT
2696 ACATGT
Bsu36I 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
CelII 2377 GCTGAGC GC^T[ACGT]AGC
2966 GCTCAGC
EarI 340 CTCTTC CTCTTC(1/4)
279 GAAGAG
Eco81I 721 CCTGAGG CC^T[ACGT]AGG
2416 CCTGAGG
EcoNI 198 CCTCGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1989 CCTGTGTCAGG
Hin1I 2973 GGCGTC G[GA]^CG[CT]C
3067 GACGTC
HincII 733 GTCGAC GT[CT]^[GA]AC
1884 GTCGAC
HindII 733 GTCGAC GT[CT]^[GA]AC
1884 GTCGAC
Ksp632I 340 CTCTTC CTCTTC(1/4)
279 GAAGAG
MseI 1076 TTAA T^TAA
2408 TTAA
NaeI 3097 GCCGGC GCC^GGC
3172 GCCGGC
NgoMIV 3097 GCCGGC G^CCGGC
3172 GCCGGC
SalI 733 GTCGAC G^TCGAC
1884 GTCGAC
SphI 2399 GCATGC GCATG^C
2894 GCATGC
TfiI 10 GAATC G^A[AT]TC
2526 GAATC
Tru9I 1076 TTAA T^TAA
2408 TTAA
Tsp509I 15 AATT ^AATT
1919 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
Sse8387I StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII