Restriction map for
pF1KSDA0758
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# NO CUT ENZYMES ( 89 enzymes) #
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Acc65I AccII AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BbeI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BspLU11I BsrBI
BssHII Bst1107I BstBI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
FseI FspI KasI KpnI KspI
MamI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 84 GTCTAC GT^[AC][GT]AC
AccIII 614 TCCGGA T^CCGGA
AclI 2071 AACGTT AA^CGTT
AocI 3591 CCTGAGG CC^T[ACGT]AGG
BalI 1409 TGGCCA TGG^CCA
BanI 443 GGCACC G^G[CT][GA]CC
BbrPI 2380 CACGTG CAC^GTG
BbvCI 3291 CCTCAGC CCTCAGC(-5/-2)
BsaAI 2380 CACGTG [CT]AC^GT[GA]
BsaI 3703 GGTCTC GGTCTC(1/5)
BsaWI 614 TCCGGA [AT]^CCGG[AT]
BseAI 614 TCCGGA T^CCGGA
Bsp1407I 753 TGTACA T^GTACA
BspEI 614 TCCGGA T^CCGGA
BsrFI 2060 GCCGGT [GA]^CCGG[CT]
BsrGI 753 TGTACA T^GTACA
BssSI 1554 CACGAG CACGAG(-5/-1)
BstAPI 2909 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3120 GGTGACC G^GT[ACGT]ACC
BstPI 3120 GGTGACC G^GT[ACGT]ACC
Bsu36I 3591 CCTGAGG CC^T[ACGT]AGG
Cfr10I 2060 GCCGGT [GA]^CCGG[CT]
DraIII 21 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 987 GACTTCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2696 CCATGG C^C[GA][CT]GG
EaeI 1409 TGGCCA [CT]^GGCC[GA]
Eco81I 3591 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3120 GGTGACC G^GT[ACGT]ACC
EcoRI 1650 GAATTC G^AATTC
EcoRV 2734 GATATC GAT^ATC
HaeII 911 GGCGCT [GA]GCGC^[CT]
HindIII 3824 AAGCTT A^AGCTT
HpaI 1843 GTTAAC GTT^AAC
MluNI 1409 TGGCCA TGG^CCA
MroI 614 TCCGGA T^CCGGA
MscI 1409 TGGCCA TGG^CCA
NcoI 2696 CCATGG C^CATGG
NspI 2002 ACATGC [GA]CATG^[CT]
PmaCI 2380 CACGTG CAC^GTG
PmlI 2380 CACGTG CAC^GTG
PshAI 321 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2071 AACGTT AA^CGTT
PvuII 122 CAGCTG CAG^CTG
ScaI 1584 AGTACT AGT^ACT
SexAI 3196 ACCTGGT A^CC[AT]GGT
SmaI 3455 CCCGGG CCC^GGG
SspBI 753 TGTACA T^GTACA
StuI 3595 AGGCCT AGG^CCT
XbaI 2661 TCTAGA T^CTAGA
XmaCI 3455 CCCGGG C^CCGGG
XmaI 3455 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 427 GACGTC GACGT^C
1675 GACGTC
AcyI 427 GACGTC G[GA]^CG[CT]C
1675 GACGTC
AspHI 357 GTGCTC G[AT]GC[AT]^C
2383 GTGCTC
AspI 859 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2941 GACAATGTC
AvaI 386 CTCGGG C^[CT]CG[GA]G
3455 CCCGGG
BamHI 231 GGATCC G^GATCC
2010 GGATCC
BanII 1959 GAGCCC G[GA]GC[CT]^C
2211 GAGCCC
BclI 2204 TGATCA T^GATCA
3539 TGATCA
BpmI 1022 CTCCAG CTGGAG(16/14)
2185 CTCCAG
BsaHI 427 GACGTC G[GA]^CG[CT]C
1675 GACGTC
BsgI 62 CTGCAC GTGCAG(16/14)
2551 GTGCAG
BsiHKAI 357 GTGCTC G[AT]GC[AT]^C
2383 GTGCTC
BsoBI 386 CTCGGG C^[CT]CG[GA]G
3455 CCCGGG
BspHI 108 TCATGA T^CATGA
557 TCATGA
CfoI 912 GCGC GCG^C
3147 GCGC
EcoT22I 218 ATGCAT ATGCA^T
1473 ATGCAT
FbaI 2204 TGATCA T^GATCA
3539 TGATCA
HgaI 2376 GACGC GACGC(5/10)
3296 GCGTC
HhaI 912 GCGC GCG^C
3147 GCGC
Hin1I 427 GACGTC G[GA]^CG[CT]C
1675 GACGTC
HinP1I 912 GCGC G^CGC
3147 GCGC
HincII 1843 GTTAAC GT[CT]^[GA]AC
3876 GTCAAC
HindII 1843 GTTAAC GT[CT]^[GA]AC
3876 GTCAAC
MspA1I 122 CAGCTG C[AC]G^C[GT]G
1690 CCGCTG
NdeI 692 CATATG CA^TATG
1807 CATATG
NsiI 218 ATGCAT ATGCA^T
1473 ATGCAT
PflFI 859 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2941 GACAATGTC
PpuMI 583 AGGTCCT [GA]G^G[AT]CC[CT]
3665 GGGTCCT
RcaI 108 TCATGA T^CATGA
557 TCATGA
SapI 3630 GAAGAGC GCTCTTC(1/4)
2291 GCTCTTC
SspI 196 AATATT AAT^ATT
1231 AATATT
Tth111I 859 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2941 GACAATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BcnI
BfaI BglI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII