Restriction map for
pF1KSDA0755
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# NO CUT ENZYMES ( 108 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BbeI
BbrPI BcgI BciVI BclI BfrI
BlnI BsaBI BsaI BseAI BsiEI
BsiWI BsmI BsoBI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstZ17I Bsu36I Cfr10I CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 1437 GACGTC GACGT^C
AccI 2771 GTCTAC GT^[AC][GT]AC
AcyI 1437 GACGTC G[GA]^CG[CT]C
AhdI 2609 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 3004 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 2609 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 218 GAGCTC G[AT]GC[AT]^C
BalI 1862 TGGCCA TGG^CCA
BanI 544 GGTGCC G^G[CT][GA]CC
BglI 129 GCCAGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 976 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 976 GCTAAGC GC^T[ACGT]AGC
BsaAI 16 TACGTG [CT]AC^GT[GA]
BsaHI 1437 GACGTC G[GA]^CG[CT]C
BsaWI 2459 ACCGGA [AT]^CCGG[AT]
BsiHKAI 218 GAGCTC G[AT]GC[AT]^C
BsmBI 1435 GAGACG CGTCTC(1/5)
BspDI 2161 ATCGAT AT^CGAT
BspMI 2706 ACCTGC ACCTGC(4/8)
BstAPI 1088 GCAACAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 232 GGTCACC G^GT[ACGT]ACC
BstPI 232 GGTCACC G^GT[ACGT]ACC
CelII 976 GCTAAGC GC^T[ACGT]AGC
CfoI 698 GCGC GCG^C
ClaI 2161 ATCGAT AT^CGAT
DraI 2380 TTTAAA TTT^AAA
EaeI 1862 TGGCCA [CT]^GGCC[GA]
Eam1105I 2609 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 232 GGTCACC G^GT[ACGT]ACC
HhaI 698 GCGC GCG^C
Hin1I 1437 GACGTC G[GA]^CG[CT]C
HinP1I 698 GCGC G^CGC
MfeI 2963 CAATTG C^AATTG
MluNI 1862 TGGCCA TGG^CCA
MscI 1862 TGGCCA TGG^CCA
MunI 2963 CAATTG C^AATTG
NcoI 2624 CCATGG C^CATGG
NheI 2316 GCTAGC G^CTAGC
SacI 218 GAGCTC GAGCT^C
SmlI 503 CTCAAG C^T[CT][GA]AG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 1412 AAATTC [GA]^AATT[CT]
2420 AAATTC
ApoI 1412 AAATTC [GA]^AATT[CT]
2420 AAATTC
Asp700I 1408 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1725 GAAGCTGTTC
BamHI 90 GGATCC G^GATCC
777 GGATCC
BanII 218 GAGCTC G[GA]GC[CT]^C
583 GGGCTC
BbvCI 43 CCTCAGC CCTCAGC(-5/-2)
1951 CCTCAGC
BcnI 175 CCGGG CC^[GC]GG
2415 CCGGG
BglII 2579 AGATCT A^GATCT
3077 AGATCT
BpmI 728 CTGGAG CTGGAG(16/14)
1961 CTGGAG
BsgI 308 CTGCAC GTGCAG(16/14)
737 CTGCAC
BspHI 1331 TCATGA T^CATGA
2618 TCATGA
BsrDI 452 CATTGC GCAATG(2/0)
2870 CATTGC
BstXI 198 CCATCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2624 CCATGGGTGTGG
DsaI 2183 CCGTGG C^C[GA][CT]GG
2624 CCATGG
EcoNI 49 CCTGGAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
628 CCTCTTCCAGG
EcoRV 656 GATATC GAT^ATC
1145 GATATC
NciI 175 CCGGG CC^[GC]GG
2415 CCGGG
NdeI 1380 CATATG CA^TATG
2443 CATATG
PstI 2304 CTGCAG CTGCA^G
2483 CTGCAG
RcaI 1331 TCATGA T^CATGA
2618 TCATGA
SapI 1423 GAAGAGC GCTCTTC(1/4)
3038 GCTCTTC
SspI 2751 AATATT AAT^ATT
2826 AATATT
StuI 57 AGGCCT AGG^CCT
1100 AGGCCT
XcmI 420 CCAGGGACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1865 CCAAGGACTGCGTGG
XmnI 1408 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1725 GAAGCTGTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaII BbsI BbvI BfaI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII