Restriction map for
pF1KSDA0736
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AviII BamHI BbrPI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 1247 GGTACC G^GTACC
AccII 1471 CGCG CG^CG
AcsI 1299 GAATTT [GA]^AATT[CT]
AcyI 348 GGCGCC G[GA]^CG[CT]C
ApoI 1299 GAATTT [GA]^AATT[CT]
Asp718I 1247 GGTACC G^GTACC
AspI 103 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1278 CCTAGG C^CTAGG
BalI 477 TGGCCA TGG^CCA
BbeI 348 GGCGCC GGCGC^C
BbvCI 2156 CCTCAGC CCTCAGC(-5/-2)
BglII 275 AGATCT A^GATCT
BlnI 1278 CCTAGG C^CTAGG
BsaHI 348 GGCGCC G[GA]^CG[CT]C
BsiEI 483 CGGCCG CG[GA][CT]^CG
BspLU11I 587 ACATGT A^CATGT
BsrBI 331 GAGCGG CCGCTC(-3/-3)
BsrFI 680 ACCGGC [GA]^CCGG[CT]
Bst1107I 1789 GTATAC GTA^TAC
BstUI 1471 CGCG CG^CG
BstXI 80 CCAAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1789 GTATAC GTA^TAC
Cfr10I 680 ACCGGC [GA]^CCGG[CT]
EagI 483 CGGCCG C^GGCCG
EclXI 483 CGGCCG C^GGCCG
Eco52I 483 CGGCCG C^GGCCG
HgaI 728 GCGTC GACGC(5/10)
Hin1I 348 GGCGCC G[GA]^CG[CT]C
KasI 348 GGCGCC G^GCGCC
KpnI 1247 GGTACC GGTAC^C
MluNI 477 TGGCCA TGG^CCA
MscI 477 TGGCCA TGG^CCA
MvnI 1471 CGCG CG^CG
NarI 348 GGCGCC GG^CGCC
PflFI 103 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1425 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2221 CTGCAG CTGCA^G
ScaI 1529 AGTACT AGT^ACT
SexAI 1243 ACCTGGT A^CC[AT]GGT
SfcI 2221 CTGCAG C^T[GA][CT]AG
SfoI 348 GGCGCC GGC^GCC
SspI 119 AATATT AAT^ATT
TaqI 1685 TCGA T^CGA
Tth111I 103 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1685 TCGA T^CGA
Van91I 1425 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 631 GTCTAC GT^[AC][GT]AC
1789 GTATAC
Aor51HI 574 AGCGCT AGC^GCT
1253 AGCGCT
BanII 1046 GGGCTC G[GA]GC[CT]^C
1068 GAGCCC
BglI 1039 GCCATTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1967 GCCTTTTTGGC
BmyI 1046 GGGCTC G[AGT]GC[ACT]^C
1068 GAGCCC
BsaBI 1218 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2100 GATCAGCATC
BsaI 17 GAGACC GGTCTC(1/5)
2203 GAGACC
Bsp1286I 1046 GGGCTC G[AGT]GC[ACT]^C
1068 GAGCCC
BsrDI 51 CATTGC GCAATG(2/0)
1986 CATTGC
BstAPI 696 GCAGTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2214 GCAGGTGCTGC
DsaI 36 CCGTGG C^C[GA][CT]GG
480 CCACGG
Eco47III 574 AGCGCT AGC^GCT
1253 AGCGCT
HincII 721 GTCAAC GT[CT]^[GA]AC
2013 GTTGAC
HindII 721 GTCAAC GT[CT]^[GA]AC
2013 GTTGAC
MamI 1218 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2100 GATCAGCATC
MseI 1190 TTAA T^TAA
1490 TTAA
SphI 72 GCATGC GCATG^C
614 GCATGC
StuI 621 AGGCCT AGG^CCT
1106 AGGCCT
Tru9I 1190 TTAA T^TAA
1490 TTAA
Tsp45I 1615 GTCAC ^GT[GC]AC
1759 GTGAC
Tsp509I 1214 AATT ^AATT
1300 AATT
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AvaI AvaII BanI BbsI
BbvI BcnI BfaI BpmI BpuAI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI BsoBI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
SmlI StyI TfiI TseI TspRI
XhoII XmaCI XmaI