Restriction map for
pF1KSDA0735
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbrPI BcgI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HgaI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1076 GGTACC G^GTACC
AccI 460 GTCTAC GT^[AC][GT]AC
AccII 61 CGCG CG^CG
Aor51HI 1082 AGCGCT AGC^GCT
ApaI 1150 GGGCCC GGGCC^C
Asp700I 996 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1076 GGTACC G^GTACC
BalI 306 TGGCCA TGG^CCA
BamHI 326 GGATCC G^GATCC
BbvCI 531 CCTCAGC CCTCAGC(-5/-2)
BclI 1835 TGATCA T^GATCA
BglII 131 AGATCT A^GATCT
BsaI 286 GAGACC GGTCTC(1/5)
BspMI 1116 GCAGGT ACCTGC(4/8)
BstUI 61 CGCG CG^CG
DraI 1390 TTTAAA TTT^AAA
DraIII 92 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1082 AGCGCT AGC^GCT
FauI 478 GCGGG CCCGC(4/6)
FbaI 1835 TGATCA T^GATCA
HapII 1652 CCGG C^CGG
HpaII 1652 CCGG C^CGG
KpnI 1076 GGTACC GGTAC^C
KspI 60 CCGCGG CCGC^GG
MaeII 1442 ACGT A^CGT
MluNI 306 TGGCCA TGG^CCA
MscI 306 TGGCCA TGG^CCA
MseI 1391 TTAA T^TAA
MspA1I 60 CCGCGG C[AC]G^C[GT]G
MspI 1652 CCGG C^CGG
MvnI 61 CGCG CG^CG
PaeR7I 2030 CTCGAG C^TCGAG
PshAI 415 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1727 CTGCAG CTGCA^G
SacII 60 CCGCGG CCGC^GG
SexAI 1072 ACCTGGT A^CC[AT]GGT
SmaI 1651 CCCGGG CCC^GGG
StuI 498 AGGCCT AGG^CCT
Tru9I 1391 TTAA T^TAA
Tsp45I 103 GTCAC ^GT[GC]AC
XcmI 775 CCACACTATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2030 CTCGAG C^TCGAG
XmaCI 1651 CCCGGG C^CCGGG
XmaI 1651 CCCGGG C^CCGGG
XmnI 996 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 416 ACATGT A^C[GA][CT]GT
1395 ACATGT
AlwNI 1786 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2038 CAGGTCCTG
BanII 897 GAGCCC G[GA]GC[CT]^C
1150 GGGCCC
BbsI 124 GAAGAC GAAGAC(2/6)
1438 GAAGAC
BciVI 155 GTATCC GTATCC(6/5)
1851 GTATCC
BcnI 1651 CCCGG CC^[GC]GG
1652 CCGGG
BfaI 448 CTAG C^TAG
913 CTAG
BpuAI 124 GAAGAC GAAGAC(2/6)
1438 GAAGAC
BseRI 262 GAGGAG GAGGAG(10/8)
695 GAGGAG
BspLU11I 416 ACATGT A^CATGT
1395 ACATGT
EarI 573 CTCTTC CTCTTC(1/4)
604 CTCTTC
EcoRI 673 GAATTC G^AATTC
1042 GAATTC
Ksp632I 573 CTCTTC CTCTTC(1/4)
604 CTCTTC
MaeI 448 CTAG C^TAG
913 CTAG
NciI 1651 CCCGG CC^[GC]GG
1652 CCGGG
PflMI 755 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
775 CCACACTATGG
PpuMI 985 GGGACCC [GA]G^G[AT]CC[CT]
2039 AGGTCCT
SfcI 1155 CTACAG C^T[GA][CT]AG
1727 CTGCAG
SfiI 180 GGCCAAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
351 GGCCCTGATGGCC
TaqI 2016 TCGA T^CGA
2031 TCGA
TthHB8I 2016 TCGA T^CGA
2031 TCGA
Van91I 755 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
775 CCACACTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanI BbeI BbvI BglI BmyI
BpmI BsaHI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
Hin1I HinP1I HinfI HphI ItaI
KasI MaeIII MboI MboII MflI
MnlI MslI MvaI MwoI NarI
NcoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI SmlI StyI TfiI
TseI Tsp509I TspRI XhoII