Restriction map for
pF1KSDA0719
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BfrI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmAI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MroI MunI
NaeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AflIII 1697 ACATGT A^C[GA][CT]GT
AlwI 1189 GATCC GGATC(4/5)
AlwNI 38 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 87 GGGCCC GGGCC^C
AvaII 301 GGTCC G^G[AT]CC
BalI 1721 TGGCCA TGG^CCA
BbeI 191 GGCGCC GGCGC^C
BbsI 249 GAAGAC GAAGAC(2/6)
BcgI 1428 CGAAGGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 889 GGATAC GTATCC(6/5)
BclI 1242 TGATCA T^GATCA
BfaI 598 CTAG C^TAG
BglII 1575 AGATCT A^GATCT
BpuAI 249 GAAGAC GAAGAC(2/6)
BsaBI 1204 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 131 CGGTCG CG[GA][CT]^CG
BspDI 1186 ATCGAT AT^CGAT
BspLU11I 1697 ACATGT A^CATGT
BspMI 1016 ACCTGC ACCTGC(4/8)
BsrI 269 CCAGT ACTGG(1/-1)
BstAPI 1143 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 795 GGATG GGATG(2/0)
BstYI 1575 AGATCT [GA]^GATC[CT]
ClaI 1186 ATCGAT AT^CGAT
DraI 373 TTTAAA TTT^AAA
DraII 300 AGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 1649 CCATGG C^C[GA][CT]GG
EcoO109I 300 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 799 GATATC GAT^ATC
FbaI 1242 TGATCA T^GATCA
FokI 795 GGATG GGATG(9/13)
HaeII 191 GGCGCC [GA]GCGC^[CT]
HgaI 217 GACGC GACGC(5/10)
HincII 448 GTTGAC GT[CT]^[GA]AC
HindII 448 GTTGAC GT[CT]^[GA]AC
HindIII 1090 AAGCTT A^AGCTT
HphI 1212 TCACC GGTGA(8/7)
KasI 191 GGCGCC G^GCGCC
MaeI 598 CTAG C^TAG
MaeII 576 ACGT A^CGT
MaeIII 628 GTCAC ^GT[ACGT]AC
MamI 1204 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1575 AGATCT [GA]^GATC[CT]
MluNI 1721 TGGCCA TGG^CCA
MscI 1721 TGGCCA TGG^CCA
NarI 191 GGCGCC GG^CGCC
NcoI 1649 CCATGG C^CATGG
NdeI 1535 CATATG CA^TATG
PpuMI 300 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1292 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 51 GAGCTC GAGCT^C
SfcI 434 CTACAG C^T[GA][CT]AG
SfiI 267 GGCCAGTCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 191 GGCGCC GGC^GCC
SmlI 1160 CTCAAG C^T[CT][GA]AG
SphI 671 GCATGC GCATG^C
SspI 368 AATATT AAT^ATT
TfiI 735 GAATC G^A[AT]TC
Tsp45I 628 GTCAC ^GT[GC]AC
XhoII 1575 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 110 CGCG CG^CG
156 CGCG
AcsI 882 AAATTC [GA]^AATT[CT]
1411 AAATTT
AcyI 191 GGCGCC G[GA]^CG[CT]C
217 GACGCC
ApoI 882 AAATTC [GA]^AATT[CT]
1411 AAATTT
AspHI 51 GAGCTC G[AT]GC[AT]^C
308 GTGCTC
AvaI 90 CCCGGG C^[CT]CG[GA]G
279 CCCGGG
BglI 108 GCCGCGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
268 GCCAGTCCGGC
BsaHI 191 GGCGCC G[GA]^CG[CT]C
217 GACGCC
BsiHKAI 51 GAGCTC G[AT]GC[AT]^C
308 GTGCTC
BsmFI 79 GGGAC GGGAC(10/14)
302 GTCCC
BsmI 946 GAATGC GAATGC(1/-1)
1197 GAATGC
BsoBI 90 CCCGGG C^[CT]CG[GA]G
279 CCCGGG
Bsp1407I 526 TGTACA T^GTACA
1127 TGTACA
BsrDI 992 CATTGC GCAATG(2/0)
1028 GCAATG
BsrGI 526 TGTACA T^GTACA
1127 TGTACA
BstUI 110 CGCG CG^CG
156 CGCG
EaeI 2 TGGCCG [CT]^GGCC[GA]
1721 TGGCCA
EarI 1362 CTCTTC CTCTTC(1/4)
1396 GAAGAG
Hin1I 191 GGCGCC G[GA]^CG[CT]C
217 GACGCC
Ksp632I 1362 CTCTTC CTCTTC(1/4)
1396 GAAGAG
MvnI 110 CGCG CG^CG
156 CGCG
PleI 177 GAGTC GAGTC(4/5)
1297 GAGTC
PvuII 118 CAGCTG CAG^CTG
1225 CAGCTG
SmaI 90 CCCGGG CCC^GGG
279 CCCGGG
SspBI 526 TGTACA T^GTACA
1127 TGTACA
TaqI 1095 TCGA T^CGA
1187 TCGA
TthHB8I 1095 TCGA T^CGA
1187 TCGA
XcmI 902 CCAAACAGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1724 CCAAATCGGAAATGG
XmaCI 90 CCCGGG C^CCGGG
279 CCCGGG
XmaI 90 CCCGGG C^CCGGG
279 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I FauI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp509I TspRI