Restriction map for
pF1KSDA0717
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# NO CUT ENZYMES ( 103 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BlnI BsaAI BsaBI BseAI
BsiEI BsiWI BsmI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwNI 130 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
BclI 969 TGATCA T^GATCA
BglI 2088 GCCCAAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1354 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1354 GCTGAGC GC^T[ACGT]AGC
BsaWI 1069 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1298 TGTACA T^GTACA
BspDI 1822 ATCGAT AT^CGAT
BspHI 1418 TCATGA T^CATGA
BsrFI 1678 ACCGGC [GA]^CCGG[CT]
BsrGI 1298 TGTACA T^GTACA
BstAPI 1640 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1354 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1678 ACCGGC [GA]^CCGG[CT]
ClaI 1822 ATCGAT AT^CGAT
FbaI 969 TGATCA T^GATCA
HaeII 1534 AGCGCC [GA]GCGC^[CT]
HgaI 230 GCGTC GACGC(5/10)
HincII 454 GTCAAC GT[CT]^[GA]AC
HindII 454 GTCAAC GT[CT]^[GA]AC
HinfI 4 GATTC G^A[ACGT]TC
MseI 1469 TTAA T^TAA
NcoI 999 CCATGG C^CATGG
PaeR7I 1363 CTCGAG C^TCGAG
PpuMI 782 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1885 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1418 TCATGA T^CATGA
SacI 1605 GAGCTC GAGCT^C
SapI 2103 GCTCTTC GCTCTTC(1/4)
SexAI 1754 ACCTGGT A^CC[AT]GGT
SmaI 1613 CCCGGG CCC^GGG
SphI 1649 GCATGC GCATG^C
SspBI 1298 TGTACA T^GTACA
TfiI 4 GATTC G^A[AT]TC
Tru9I 1469 TTAA T^TAA
Tsp509I 337 AATT ^AATT
XhoI 1363 CTCGAG C^TCGAG
XmaCI 1613 CCCGGG C^CCGGG
XmaI 1613 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccII 100 CGCG CG^CG
795 CGCG
AflIII 1037 ACGTGT A^C[GA][CT]GT
1922 ACGTGT
AlwI 1207 GATCC GGATC(4/5)
1106 GGATC
ApaI 918 GGGCCC GGGCC^C
1270 GGGCCC
AspHI 395 GAGCAC G[AT]GC[AT]^C
1605 GAGCTC
AviII 827 TGCGCA TGC^GCA
1379 TGCGCA
BbsI 1158 GTCTTC GAAGAC(2/6)
2130 GTCTTC
BglII 295 AGATCT A^GATCT
845 AGATCT
BpuAI 1158 GTCTTC GAAGAC(2/6)
2130 GTCTTC
BsiHKAI 395 GAGCAC G[AT]GC[AT]^C
1605 GAGCTC
BsmBI 231 CGTCTC CGTCTC(1/5)
209 GAGACG
BspMI 428 ACCTGC ACCTGC(4/8)
638 ACCTGC
BsrBI 787 CCGCTC CCGCTC(-3/-3)
820 GAGCGG
BstUI 100 CGCG CG^CG
795 CGCG
DsaI 71 CCGTGG C^C[GA][CT]GG
999 CCATGG
EarI 2104 CTCTTC CTCTTC(1/4)
1192 GAAGAG
FspI 827 TGCGCA TGC^GCA
1379 TGCGCA
Ksp632I 2104 CTCTTC CTCTTC(1/4)
1192 GAAGAG
MvnI 100 CGCG CG^CG
795 CGCG
StuI 1412 AGGCCT AGG^CCT
1439 AGGCCT
TspRI 1781 CAGTG CA[GC]TG[ACGT][ACGT]^
1864 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AatII Acc65I AciI AcyI AfaI
AluI Asp718I AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI KpnI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TthHB8I Van91I XcmI XhoII