Restriction map for
pF1KSDA0693
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AhdI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstPI BstXI
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI MaeI MamI MfeI MluI
MroI MseI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PmeI PshAI PshBI Psp1406I PvuII
RcaI RsrII SacI SalI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 947 GGTACC G^GTACC
AccI 175 GTATAC GT^[AC][GT]AC
AgeI 875 ACCGGT A^CCGGT
Alw44I 138 GTGCAC G^TGCAC
ApaLI 138 GTGCAC G^TGCAC
Asp718I 947 GGTACC G^GTACC
AvaI 1015 CCCGGG C^[CT]CG[GA]G
BalI 182 TGGCCA TGG^CCA
BbrPI 385 CACGTG CAC^GTG
BsoBI 1015 CCCGGG C^[CT]CG[GA]G
BsrBI 276 GAGCGG CCGCTC(-3/-3)
Bst1107I 175 GTATAC GTA^TAC
BstYI 155 AGATCC [GA]^GATC[CT]
BstZ17I 175 GTATAC GTA^TAC
DrdI 1119 GACAGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 131 CGGCCG C^GGCCG
EarI 723 CTCTTC CTCTTC(1/4)
EclXI 131 CGGCCG C^GGCCG
Eco52I 131 CGGCCG C^GGCCG
KpnI 947 GGTACC GGTAC^C
Ksp632I 723 CTCTTC CTCTTC(1/4)
KspI 788 CCGCGG CCGC^GG
MflI 155 AGATCC [GA]^GATC[CT]
MluNI 182 TGGCCA TGG^CCA
MscI 182 TGGCCA TGG^CCA
PinAI 875 ACCGGT A^CCGGT
PmaCI 385 CACGTG CAC^GTG
PmlI 385 CACGTG CAC^GTG
PpuMI 465 GGGACCC [GA]G^G[AT]CC[CT]
PstI 355 CTGCAG CTGCA^G
PvuI 188 CGATCG CGAT^CG
SacII 788 CCGCGG CCGC^GG
SapI 722 GCTCTTC GCTCTTC(1/4)
SbfI 354 CCTGCAGG CCTGCA^GG
SexAI 170 ACCTGGT A^CC[AT]GGT
SmaI 1015 CCCGGG CCC^GGG
Sse8387I 354 CCTGCAGG CCTGCA^GG
TaqI 884 TCGA T^CGA
Tsp509I 1177 AATT ^AATT
TspRI 97 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 884 TCGA T^CGA
XcmI 638 CCATCGCCATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 155 AGATCC [GA]^GATC[CT]
XmaCI 1015 CCCGGG C^CCGGG
XmaI 1015 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 386 ACGTGT A^C[GA][CT]GT
518 ACGTGT
AlwI 93 GATCC GGATC(4/5)
156 GATCC
ApaI 255 GGGCCC GGGCC^C
445 GGGCCC
AspHI 138 GTGCAC G[AT]GC[AT]^C
1109 GAGCAC
BglI 362 GCCAGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
699 GCCCTACCGGC
BsaAI 385 CACGTG [CT]AC^GT[GA]
517 TACGTG
BsaWI 164 ACCGGA [AT]^CCGG[AT]
875 ACCGGT
BsgI 160 CTGCAC GTGCAG(16/14)
295 CTGCAC
BsiHKAI 138 GTGCAC G[AT]GC[AT]^C
1109 GAGCAC
BsmAI 522 GTCTC GTCTC(1/5)
556 GTCTC
BsrFI 704 ACCGGC [GA]^CCGG[CT]
875 ACCGGT
BstAPI 153 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
213 GCAGTCCCTGC
Cfr10I 704 ACCGGC [GA]^CCGG[CT]
875 ACCGGT
EcoNI 351 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
738 CCTGGGCCAGG
MboII 66 GAAGA GAAGA(8/7)
724 TCTTC
NspI 209 ACATGC [GA]CATG^[CT]
539 ACATGC
ScaI 752 AGTACT AGT^ACT
809 AGTACT
Tsp45I 317 GTGAC ^GT[GC]AC
1056 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI KasI MaeII MaeIII MboI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI StyI TseI