Restriction map for
pF1KSDA0690
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# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AgeI AhdI AscI
AseI AsnI AspEI AvrII BbeI
BbrPI BcgI BciVI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI Bsp1407I
BspDI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI Eam1105I EcoRV EcoT22I HpaI
KasI KspI MamI MfeI MluI
MunI NarI NdeI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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Acc65I 277 GGTACC G^GTACC
AccI 3584 GTCGAC GT^[AC][GT]AC
AcyI 228 GACGCC G[GA]^CG[CT]C
AflII 871 CTTAAG C^TTAAG
AflIII 2640 ACATGT A^C[GA][CT]GT
Alw44I 2292 GTGCAC G^TGCAC
ApaLI 2292 GTGCAC G^TGCAC
Asp700I 2715 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 277 GGTACC G^GTACC
AviII 1694 TGCGCA TGC^GCA
BfrI 871 CTTAAG C^TTAAG
BsaHI 228 GACGCC G[GA]^CG[CT]C
BsaI 1421 GAGACC GGTCTC(1/5)
BspHI 698 TCATGA T^CATGA
BspLU11I 2640 ACATGT A^CATGT
BssSI 2341 CTCGTG CACGAG(-5/-1)
BstAPI 1344 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2491 TTTAAA TTT^AAA
EcoRI 502 GAATTC G^AATTC
FseI 3436 GGCCGGCC GGCCGG^CC
FspI 1694 TGCGCA TGC^GCA
Hin1I 228 GACGCC G[GA]^CG[CT]C
KpnI 277 GGTACC GGTAC^C
NheI 861 GCTAGC G^CTAGC
PshAI 2442 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 698 TCATGA T^CATGA
SalI 3584 GTCGAC G^TCGAC
ScaI 437 AGTACT AGT^ACT
XcmI 759 CCACGCCAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2715 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 44 enzymes) #
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AccII 1439 CGCG CG^CG
2436 CGCG
AccIII 455 TCCGGA T^CCGGA
2804 TCCGGA
AcsI 502 GAATTC [GA]^AATT[CT]
1144 AAATTC
Aor51HI 47 AGCGCT AGC^GCT
332 AGCGCT
ApaI 2109 GGGCCC GGGCC^C
2117 GGGCCC
ApoI 502 GAATTC [GA]^AATT[CT]
1144 AAATTC
AspI 1963 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2569 GACGTGGTC
BamHI 3063 GGATCC G^GATCC
3444 GGATCC
BbsI 2154 GAAGAC GAAGAC(2/6)
1186 GTCTTC
BbvCI 2812 GCTGAGG CCTCAGC(-5/-2)
3301 GCTGAGG
BclI 3161 TGATCA T^GATCA
3254 TGATCA
BglII 368 AGATCT A^GATCT
1538 AGATCT
BpuAI 2154 GAAGAC GAAGAC(2/6)
1186 GTCTTC
BsaWI 455 TCCGGA [AT]^CCGG[AT]
2804 TCCGGA
BseAI 455 TCCGGA T^CCGGA
2804 TCCGGA
BsiEI 130 CGGCCG CG[GA][CT]^CG
3439 CGGCCG
BsmBI 226 GAGACG CGTCTC(1/5)
424 GAGACG
BsmI 1033 GAATGC GAATGC(1/-1)
3353 GCATTC
BspEI 455 TCCGGA T^CCGGA
2804 TCCGGA
BstUI 1439 CGCG CG^CG
2436 CGCG
DrdI 148 GACCTGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1666 GACCTGAGGGTC
EagI 130 CGGCCG C^GGCCG
3439 CGGCCG
EclXI 130 CGGCCG C^GGCCG
3439 CGGCCG
Eco47III 47 AGCGCT AGC^GCT
332 AGCGCT
Eco52I 130 CGGCCG C^GGCCG
3439 CGGCCG
FbaI 3161 TGATCA T^GATCA
3254 TGATCA
HincII 2797 GTCAAC GT[CT]^[GA]AC
3584 GTCGAC
HindII 2797 GTCAAC GT[CT]^[GA]AC
3584 GTCGAC
HindIII 2671 AAGCTT A^AGCTT
2707 AAGCTT
MroI 455 TCCGGA T^CCGGA
2804 TCCGGA
MseI 872 TTAA T^TAA
2492 TTAA
MvnI 1439 CGCG CG^CG
2436 CGCG
PflFI 1963 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2569 GACGTGGTC
PflMI 711 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1296 CCAGGCAGTGG
SacI 793 GAGCTC GAGCT^C
1161 GAGCTC
SapI 669 GAAGAGC GCTCTTC(1/4)
1488 GAAGAGC
SexAI 899 ACCTGGT A^CC[AT]GGT
1964 ACCTGGT
SmaI 2113 CCCGGG CCC^GGG
2819 CCCGGG
TfiI 402 GATTC G^A[AT]TC
1531 GAATC
Tru9I 872 TTAA T^TAA
2492 TTAA
Tth111I 1963 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2569 GACGTGGTC
Van91I 711 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1296 CCAGGCAGTGG
XmaCI 2113 CCCGGG C^CCGGG
2819 CCCGGG
XmaI 2113 CCCGGG C^CCGGG
2819 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII