Restriction map for
pF1KSDA0688
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BbrPI BcgI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsiWI Bsp1407I BspDI BspLU11I
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV FbaI FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SbfI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tru9I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 405 TCCGGA T^CCGGA
AcsI 2080 AAATTC [GA]^AATT[CT]
ApoI 2080 AAATTC [GA]^AATT[CT]
AspI 2307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 866 TGCGCA TGC^GCA
BamHI 1194 GGATCC G^GATCC
BbsI 1096 GTCTTC GAAGAC(2/6)
BbvCI 140 CCTCAGC CCTCAGC(-5/-2)
BciVI 2092 GGATAC GTATCC(6/5)
BlpI 1730 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1730 GCTCAGC GC^T[ACGT]AGC
BpuAI 1096 GTCTTC GAAGAC(2/6)
BseAI 405 TCCGGA T^CCGGA
BsiEI 2410 CGGCCG CG[GA][CT]^CG
BsmBI 268 GAGACG CGTCTC(1/5)
BsmI 694 GCATTC GAATGC(1/-1)
BspEI 405 TCCGGA T^CCGGA
BspHI 1080 TCATGA T^CATGA
BsrDI 1292 CATTGC GCAATG(2/0)
BssSI 1641 CACGAG CACGAG(-5/-1)
CelII 1730 GCTCAGC GC^T[ACGT]AGC
EagI 2410 CGGCCG C^GGCCG
EclXI 2410 CGGCCG C^GGCCG
Eco52I 2410 CGGCCG C^GGCCG
EcoNI 333 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 866 TGCGCA TGC^GCA
KspI 2117 CCGCGG CCGC^GG
MaeII 584 ACGT A^CGT
MroI 405 TCCGGA T^CCGGA
NspI 1103 ACATGC [GA]CATG^[CT]
PflFI 2307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1080 TCATGA T^CATGA
SacII 2117 CCGCGG CCGC^GG
ScaI 1670 AGTACT AGT^ACT
SspI 1546 AATATT AAT^ATT
TaqI 311 TCGA T^CGA
TfiI 2469 GATTC G^A[AT]TC
Tsp509I 2081 AATT ^AATT
Tth111I 2307 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 311 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1747 CGCG CG^CG
2118 CGCG
AcyI 342 GGCGCC G[GA]^CG[CT]C
2489 GGCGCC
AocI 904 CCTGAGG CC^T[ACGT]AGG
1198 CCTGAGG
Aor51HI 632 AGCGCT AGC^GCT
716 AGCGCT
BbeI 342 GGCGCC GGCGC^C
2489 GGCGCC
BglI 145 GCCCGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1721 GCCCAACTGGC
BsaHI 342 GGCGCC G[GA]^CG[CT]C
2489 GGCGCC
BsrBI 88 CCGCTC CCGCTC(-3/-3)
1698 CCGCTC
BstAPI 1475 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2024 GCATGGTGTGC
BstUI 1747 CGCG CG^CG
2118 CGCG
BstXI 1422 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2243 CCACAGATGTGG
Bsu36I 904 CCTGAGG CC^T[ACGT]AGG
1198 CCTGAGG
Eco47III 632 AGCGCT AGC^GCT
716 AGCGCT
Eco81I 904 CCTGAGG CC^T[ACGT]AGG
1198 CCTGAGG
EcoT22I 1128 ATGCAT ATGCA^T
1968 ATGCAT
HgaI 270 GACGC GACGC(5/10)
209 GCGTC
Hin1I 342 GGCGCC G[GA]^CG[CT]C
2489 GGCGCC
KasI 342 GGCGCC G^GCGCC
2489 GGCGCC
MvnI 1747 CGCG CG^CG
2118 CGCG
NarI 342 GGCGCC GG^CGCC
2489 GGCGCC
NsiI 1128 ATGCAT ATGCA^T
1968 ATGCAT
PstI 1218 CTGCAG CTGCA^G
2294 CTGCAG
PvuII 1348 CAGCTG CAG^CTG
1384 CAGCTG
SapI 623 GAAGAGC GCTCTTC(1/4)
2459 GAAGAGC
SfoI 342 GGCGCC GGC^GCC
2489 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TseI
Tsp45I TspRI Van91I XhoII XmaCI
XmaI