Restriction map for
pF1KSDA0662
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# NO CUT ENZYMES ( 80 enzymes) #
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AccIII AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
AspEI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BseAI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI DraI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoRV FbaI FspI
HindIII HpaI KspI MamI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PinAI PmeI
PshBI Psp1406I RcaI SacII SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TfiI XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 773 GGTACC G^GTACC
AflIII 2036 ACGTGT A^C[GA][CT]GT
AlwNI 3095 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 947 CCTCAGG CC^T[ACGT]AGG
Aor51HI 845 AGCGCT AGC^GCT
ApaI 3116 GGGCCC GGGCC^C
Asp718I 773 GGTACC G^GTACC
AspI 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 1539 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 2791 GAGACC GGTCTC(1/5)
BsaWI 1350 ACCGGA [AT]^CCGG[AT]
BsiWI 3223 CGTACG C^GTACG
BssHII 2324 GCGCGC G^CGCGC
BstXI 608 CCAGCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 947 CCTCAGG CC^T[ACGT]AGG
CpoI 84 CGGACCG CG^G[AT]CCG
EagI 2241 CGGCCG C^GGCCG
EclXI 2241 CGGCCG C^GGCCG
Eco47III 845 AGCGCT AGC^GCT
Eco52I 2241 CGGCCG C^GGCCG
Eco81I 947 CCTCAGG CC^T[ACGT]AGG
EcoT22I 674 ATGCAT ATGCA^T
FseI 815 GGCCGGCC GGCCGG^CC
KpnI 773 GGTACC GGTAC^C
NdeI 1043 CATATG CA^TATG
NsiI 674 ATGCAT ATGCA^T
NspI 1742 ACATGC [GA]CATG^[CT]
PaeR7I 583 CTCGAG C^TCGAG
PflFI 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3138 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 563 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 483 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1219 CGATCG CGAT^CG
RsrII 84 CGGACCG CG^G[AT]CCG
SbfI 2409 CCTGCAGG CCTGCA^GG
ScaI 704 AGTACT AGT^ACT
SexAI 890 ACCAGGT A^CC[AT]GGT
Sse8387I 2409 CCTGCAGG CCTGCA^GG
Tth111I 889 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3138 CCAGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 583 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AatII 232 GACGTC GACGT^C
442 GACGTC
AccI 895 GTCGAC GT^[AC][GT]AC
2560 GTCGAC
AlwI 1314 GATCC GGATC(4/5)
956 GGATC
Asp700I 795 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAAGCCATTC
BbeI 123 GGCGCC GGCGC^C
760 GGCGCC
BbrPI 778 CACGTG CAC^GTG
2439 CACGTG
BsmBI 1418 CGTCTC CGTCTC(1/5)
3188 CGTCTC
BsrDI 677 CATTGC GCAATG(2/0)
2246 GCAATG
BstYI 956 GGATCT [GA]^GATC[CT]
1313 AGATCC
HgaI 2701 GACGC GACGC(5/10)
23 GCGTC
HincII 895 GTCGAC GT[CT]^[GA]AC
2560 GTCGAC
HindII 895 GTCGAC GT[CT]^[GA]AC
2560 GTCGAC
KasI 123 GGCGCC G^GCGCC
760 GGCGCC
MflI 956 GGATCT [GA]^GATC[CT]
1313 AGATCC
MseI 184 TTAA T^TAA
2666 TTAA
NarI 123 GGCGCC GG^CGCC
760 GGCGCC
PmaCI 778 CACGTG CAC^GTG
2439 CACGTG
PmlI 778 CACGTG CAC^GTG
2439 CACGTG
PvuII 1168 CAGCTG CAG^CTG
2282 CAGCTG
SalI 895 GTCGAC G^TCGAC
2560 GTCGAC
SapI 2581 GAAGAGC GCTCTTC(1/4)
261 GCTCTTC
SfoI 123 GGCGCC GGC^GCC
760 GGCGCC
Tru9I 184 TTAA T^TAA
2666 TTAA
XhoII 956 GGATCT [GA]^GATC[CT]
1313 AGATCC
XmnI 795 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAAGCCATTC
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# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI ApoI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XmaCI XmaI