Restriction map for
pF1KSDA0655
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbrPI BcgI BclI BfrI BlnI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII ScaI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 320 GGTACC G^GTACC
AcsI 2578 GAATTT [GA]^AATT[CT]
AcyI 2050 GACGCC G[GA]^CG[CT]C
AgeI 752 ACCGGT A^CCGGT
Alw44I 1378 GTGCAC G^TGCAC
ApaLI 1378 GTGCAC G^TGCAC
ApoI 2578 GAATTT [GA]^AATT[CT]
Asp700I 783 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 320 GGTACC G^GTACC
BalI 3065 TGGCCA TGG^CCA
BamHI 2465 GGATCC G^GATCC
BbsI 2973 GAAGAC GAAGAC(2/6)
BglII 419 AGATCT A^GATCT
BpuAI 2973 GAAGAC GAAGAC(2/6)
BsaAI 3025 TACGTG [CT]AC^GT[GA]
BsaHI 2050 GACGCC G[GA]^CG[CT]C
BsaI 347 GAGACC GGTCTC(1/5)
BsaWI 752 ACCGGT [AT]^CCGG[AT]
BsiEI 2154 CGACCG CG[GA][CT]^CG
BsmI 1164 GAATGC GAATGC(1/-1)
DraIII 3022 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
Hin1I 2050 GACGCC G[GA]^CG[CT]C
HindIII 562 AAGCTT A^AGCTT
KpnI 320 GGTACC GGTAC^C
MluNI 3065 TGGCCA TGG^CCA
MscI 3065 TGGCCA TGG^CCA
NcoI 2894 CCATGG C^CATGG
PinAI 752 ACCGGT A^CCGGT
PshAI 498 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2347 GTCGAC G^TCGAC
SexAI 1967 ACCTGGT A^CC[AT]GGT
SfiI 1980 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2189 CCCGGG CCC^GGG
SphI 2993 GCATGC GCATG^C
Tsp509I 2579 AATT ^AATT
XmaCI 2189 CCCGGG C^CCGGG
XmaI 2189 CCCGGG C^CCGGG
XmnI 783 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 394 GTCTAC GT^[AC][GT]AC
2347 GTCGAC
AocI 620 CCTCAGG CC^T[ACGT]AGG
777 CCTCAGG
BbvCI 875 CCTCAGC CCTCAGC(-5/-2)
1254 GCTGAGG
BciVI 2100 GGATAC GTATCC(6/5)
607 GTATCC
BsgI 1936 CTGCAC GTGCAG(16/14)
2478 CTGCAC
BsmBI 284 GAGACG CGTCTC(1/5)
2944 GAGACG
BssHII 1333 GCGCGC G^CGCGC
1589 GCGCGC
Bsu36I 620 CCTCAGG CC^T[ACGT]AGG
777 CCTCAGG
Eco81I 620 CCTCAGG CC^T[ACGT]AGG
777 CCTCAGG
HaeII 1746 GGCGCT [GA]GCGC^[CT]
1876 GGCGCT
HincII 505 GTCAAC GT[CT]^[GA]AC
2347 GTCGAC
HindII 505 GTCAAC GT[CT]^[GA]AC
2347 GTCGAC
MslI 292 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
361 CATTTGCATG
NaeI 21 GCCGGC GCC^GGC
1799 GCCGGC
NgoMIV 21 GCCGGC G^CCGGC
1799 GCCGGC
PleI 1504 GAGTC GAGTC(4/5)
1816 GAGTC
SapI 1152 GAAGAGC GCTCTTC(1/4)
1527 GAAGAGC
SmlI 433 CTCAAG C^T[CT][GA]AG
1549 CTCAAG
StuI 1988 AGGCCT AGG^CCT
2610 AGGCCT
TfiI 571 GAATC G^A[AT]TC
948 GAATC
XcmI 2123 CCACCTCGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2648 CCACACAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I SbfI ScrFI SfaNI SfcI
Sse8387I StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoII