Restriction map for
pF1KSDA0649
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AscI AseI AsnI
Asp718I AspI AviII AvrII BbrPI
BbvCI BciVI BclI BfrI BglII
BlnI BsaAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI KspI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AcsI 771 AAATTC [GA]^AATT[CT]
Aor51HI 1024 AGCGCT AGC^GCT
ApoI 771 AAATTC [GA]^AATT[CT]
BbsI 2041 GAAGAC GAAGAC(2/6)
BcgI 168 CGACGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 2041 GAAGAC GAAGAC(2/6)
BsaBI 144 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3063 CGACCG CG[GA][CT]^CG
BssHII 198 GCGCGC G^CGCGC
BstAPI 831 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1292 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 1024 AGCGCT AGC^GCT
HincII 3526 GTCAAC GT[CT]^[GA]AC
HindII 3526 GTCAAC GT[CT]^[GA]AC
MamI 144 GATGCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 2101 TTAA T^TAA
NspI 1979 GCATGT [GA]CATG^[CT]
PshAI 1303 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 453 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2119 CTCAAG C^T[CT][GA]AG
StuI 50 AGGCCT AGG^CCT
Tru9I 2101 TTAA T^TAA
Tsp509I 772 AATT ^AATT
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 1613 TCCGGA T^CCGGA
2213 TCCGGA
AhdI 3178 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACGAAGGGTC
Alw44I 265 GTGCAC G^TGCAC
2664 GTGCAC
AlwNI 694 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
832 CAGGGCCTG
AocI 912 CCTCAGG CC^T[ACGT]AGG
3507 CCTGAGG
ApaLI 265 GTGCAC G^TGCAC
2664 GTGCAC
AspEI 3178 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACGAAGGGTC
BamHI 607 GGATCC G^GATCC
2802 GGATCC
BbeI 2015 GGCGCC GGCGC^C
2974 GGCGCC
BseAI 1613 TCCGGA T^CCGGA
2213 TCCGGA
BspEI 1613 TCCGGA T^CCGGA
2213 TCCGGA
BspHI 8 TCATGA T^CATGA
1049 TCATGA
BspMI 1160 ACCTGC ACCTGC(4/8)
1907 ACCTGC
BssSI 2897 CACGAG CACGAG(-5/-1)
1413 CTCGTG
BstYI 607 GGATCC [GA]^GATC[CT]
2802 GGATCC
Bsu36I 912 CCTCAGG CC^T[ACGT]AGG
3507 CCTGAGG
Eam1105I 3178 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACGAAGGGTC
Eco81I 912 CCTCAGG CC^T[ACGT]AGG
3507 CCTGAGG
KasI 2015 GGCGCC G^GCGCC
2974 GGCGCC
MflI 607 GGATCC [GA]^GATC[CT]
2802 GGATCC
MroI 1613 TCCGGA T^CCGGA
2213 TCCGGA
MslI 532 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1974 CACTCGCATG
NarI 2015 GGCGCC GG^CGCC
2974 GGCGCC
NcoI 320 CCATGG C^CATGG
1298 CCATGG
PflMI 2650 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3110 CCAGCCCGTGG
RcaI 8 TCATGA T^CATGA
1049 TCATGA
SacI 2034 GAGCTC GAGCT^C
3477 GAGCTC
SapI 2250 GAAGAGC GCTCTTC(1/4)
3255 GCTCTTC
SfoI 2015 GGCGCC GGC^GCC
2974 GGCGCC
Van91I 2650 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3110 CCAGCCCGTGG
XhoII 607 GGATCC [GA]^GATC[CT]
2802 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XmaCI XmaI XmnI