Restriction map for
pF1KSDA0640
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AgeI AhdI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HaeII
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacI SacII SalI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tsp45I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 977 TCCGGA T^CCGGA
AcsI 265 GAATTC [GA]^AATT[CT]
AflII 427 CTTAAG C^TTAAG
AflIII 1271 ACATGT A^C[GA][CT]GT
Alw44I 117 GTGCAC G^TGCAC
AlwI 735 GGATC GGATC(4/5)
ApaLI 117 GTGCAC G^TGCAC
ApoI 265 GAATTC [GA]^AATT[CT]
AspI 582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 387 GTATCC GTATCC(6/5)
BclI 1670 TGATCA T^GATCA
BcnI 1316 CCCGG CC^[GC]GG
BfaI 1390 CTAG C^TAG
BfrI 427 CTTAAG C^TTAAG
BsaI 78 GGTCTC GGTCTC(1/5)
BsaWI 977 TCCGGA [AT]^CCGG[AT]
BseAI 977 TCCGGA T^CCGGA
BsgI 1103 GTGCAG GTGCAG(16/14)
BspEI 977 TCCGGA T^CCGGA
BspLU11I 1271 ACATGT A^CATGT
BsrFI 575 ACCGGC [GA]^CCGG[CT]
BssSI 1100 CTCGTG CACGAG(-5/-1)
BstAPI 1096 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1352 CCAGGTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 735 GGATCT [GA]^GATC[CT]
Cfr10I 575 ACCGGC [GA]^CCGG[CT]
EcoNI 1485 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 265 GAATTC G^AATTC
EcoT14I 1496 CCATGG C^C[AT][AT]GG
EcoT22I 341 ATGCAT ATGCA^T
FauI 956 CCCGC CCCGC(4/6)
FbaI 1670 TGATCA T^GATCA
HgaI 963 GCGTC GACGC(5/10)
MaeI 1390 CTAG C^TAG
MaeIII 644 GTTAC ^GT[ACGT]AC
MflI 735 GGATCT [GA]^GATC[CT]
MroI 977 TCCGGA T^CCGGA
NciI 1316 CCCGG CC^[GC]GG
NcoI 1496 CCATGG C^CATGG
NsiI 341 ATGCAT ATGCA^T
PflFI 582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1592 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 157 GAAGAGC GCTCTTC(1/4)
SbfI 1485 CCTGCAGG CCTGCA^GG
SexAI 1647 ACCAGGT A^CC[AT]GGT
Sse8387I 1485 CCTGCAGG CCTGCA^GG
StyI 1496 CCATGG C^C[AT][AT]GG
Tth111I 582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1592 CCAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1496 CCATGGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 735 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwNI 1057 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1480 CAGGCCCTG
AspHI 117 GTGCAC G[AT]GC[AT]^C
160 GAGCAC
BbsI 1267 GAAGAC GAAGAC(2/6)
1371 GTCTTC
BmyI 117 GTGCAC G[AGT]GC[ACT]^C
160 GAGCAC
BpuAI 1267 GAAGAC GAAGAC(2/6)
1371 GTCTTC
BsiHKAI 117 GTGCAC G[AT]GC[AT]^C
160 GAGCAC
BsmFI 1683 GGGAC GGGAC(10/14)
87 GTCCC
Bsp1286I 117 GTGCAC G[AGT]GC[ACT]^C
160 GAGCAC
BspHI 138 TCATGA T^CATGA
1620 TCATGA
BspMI 422 ACCTGC ACCTGC(4/8)
1686 ACCTGC
DdeI 377 CTGAG C^T[ACGT]AG
1700 CTGAG
DpnII 736 GATC ^GATC
1671 GATC
DraI 216 TTTAAA TTT^AAA
346 TTTAAA
DsaI 1496 CCATGG C^C[GA][CT]GG
1748 CCACGG
HphI 47 TCACC GGTGA(8/7)
1665 TCACC
MboI 736 GATC ^GATC
1671 GATC
NdeII 736 GATC ^GATC
1671 GATC
NspI 206 ACATGC [GA]CATG^[CT]
1271 ACATGT
PpuMI 98 AGGTCCT [GA]G^G[AT]CC[CT]
1683 GGGACCT
PvuII 988 CAGCTG CAG^CTG
1504 CAGCTG
RcaI 138 TCATGA T^CATGA
1620 TCATGA
Sau3AI 736 GATC ^GATC
1671 GATC
SfaNI 340 GATGC GCATC(5/9)
1096 GCATC
TaqI 56 TCGA T^CGA
1635 TCGA
TthHB8I 56 TCGA T^CGA
1635 TCGA
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AseI AsnI
AvaII BbvI BpmI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
ItaI Ksp632I MboII MnlI MseI
MspA1I MspI MvaI MwoI NlaIII
NlaIV PleI PshBI PstI RsaI
Sau96I ScrFI SfcI SmlI TfiI
Tru9I TseI Tsp509I TspRI