Restriction map for
pF1KSDA0632
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI AspEI
AspI BamHI BbvCI BcgI BfrI
BglII BlpI Bpu1102I BsaBI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KspI MamI MfeI MflI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1919 GGTACC G^GTACC
AccI 1712 GTCTAC GT^[AC][GT]AC
AgeI 1916 ACCGGT A^CCGGT
AocI 786 CCTTAGG CC^T[ACGT]AGG
ApaI 35 GGGCCC GGGCC^C
Asp700I 570 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1919 GGTACC G^GTACC
AviII 778 TGCGCA TGC^GCA
AvrII 999 CCTAGG C^CTAGG
BalI 1682 TGGCCA TGG^CCA
BbeI 129 GGCGCC GGCGC^C
BbrPI 832 CACGTG CAC^GTG
BciVI 1242 GGATAC GTATCC(6/5)
BclI 437 TGATCA T^GATCA
BlnI 999 CCTAGG C^CTAGG
BsaAI 832 CACGTG [CT]AC^GT[GA]
BstAPI 705 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 786 CCTTAGG CC^T[ACGT]AGG
DpnII 438 GATC ^GATC
EarI 1587 GAAGAG CTCTTC(1/4)
Eco81I 786 CCTTAGG CC^T[ACGT]AGG
FbaI 437 TGATCA T^GATCA
FspI 778 TGCGCA TGC^GCA
HincII 1318 GTCAAC GT[CT]^[GA]AC
HindII 1318 GTCAAC GT[CT]^[GA]AC
KasI 129 GGCGCC G^GCGCC
KpnI 1919 GGTACC GGTAC^C
Ksp632I 1587 GAAGAG CTCTTC(1/4)
MboI 438 GATC ^GATC
MluNI 1682 TGGCCA TGG^CCA
MscI 1682 TGGCCA TGG^CCA
MseI 1568 TTAA T^TAA
NarI 129 GGCGCC GG^CGCC
NdeII 438 GATC ^GATC
NspI 1137 GCATGT [GA]CATG^[CT]
PflMI 377 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1916 ACCGGT A^CCGGT
PmaCI 832 CACGTG CAC^GTG
PmlI 832 CACGTG CAC^GTG
SacI 730 GAGCTC GAGCT^C
SapI 1587 GAAGAGC GCTCTTC(1/4)
Sau3AI 438 GATC ^GATC
SexAI 1541 ACCAGGT A^CC[AT]GGT
SfoI 129 GGCGCC GGC^GCC
SmaI 1333 CCCGGG CCC^GGG
StuI 572 AGGCCT AGG^CCT
Tru9I 1568 TTAA T^TAA
Tsp509I 380 AATT ^AATT
Van91I 377 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1130 CCATGCTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1333 CCCGGG C^CCGGG
XmaI 1333 CCCGGG C^CCGGG
XmnI 570 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 753 CGCG CG^CG
1785 CGCG
AccIII 1203 TCCGGA T^CCGGA
2030 TCCGGA
AcyI 129 GGCGCC G[GA]^CG[CT]C
622 GACGCC
BbsI 241 GAAGAC GAAGAC(2/6)
888 GAAGAC
BfaI 937 CTAG C^TAG
1000 CTAG
BglI 85 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1409 GCCTGGAGGGC
BpuAI 241 GAAGAC GAAGAC(2/6)
888 GAAGAC
BsaHI 129 GGCGCC G[GA]^CG[CT]C
622 GACGCC
BseAI 1203 TCCGGA T^CCGGA
2030 TCCGGA
BspEI 1203 TCCGGA T^CCGGA
2030 TCCGGA
BstEII 1371 GGTGACC G^GT[ACGT]ACC
1746 GGTGACC
BstPI 1371 GGTGACC G^GT[ACGT]ACC
1746 GGTGACC
BstUI 753 CGCG CG^CG
1785 CGCG
EcoO65I 1371 GGTGACC G^GT[ACGT]ACC
1746 GGTGACC
HgaI 622 GACGC GACGC(5/10)
174 GCGTC
Hin1I 129 GGCGCC G[GA]^CG[CT]C
622 GACGCC
MaeI 937 CTAG C^TAG
1000 CTAG
MaeII 647 ACGT A^CGT
833 ACGT
MroI 1203 TCCGGA T^CCGGA
2030 TCCGGA
MvnI 753 CGCG CG^CG
1785 CGCG
NcoI 44 CCATGG C^CATGG
512 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I