Restriction map for
pF1KSDA0626
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
CpoI DraII DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HincII HindII HindIII HpaI HpaII
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 1131 GGTACC G^GTACC
AccII 421 CGCG CG^CG
AcsI 804 GAATTT [GA]^AATT[CT]
AcyI 910 GACGCC G[GA]^CG[CT]C
AlwI 3 GGATC GGATC(4/5)
ApoI 804 GAATTT [GA]^AATT[CT]
Asp718I 1131 GGTACC G^GTACC
BanII 129 GGGCTC G[GA]GC[CT]^C
BbrPI 369 CACGTG CAC^GTG
BbsI 540 GAAGAC GAAGAC(2/6)
BbvCI 934 CCTCAGC CCTCAGC(-5/-2)
BpuAI 540 GAAGAC GAAGAC(2/6)
BsaAI 369 CACGTG [CT]AC^GT[GA]
BsaHI 910 GACGCC G[GA]^CG[CT]C
BsiYI 839 CCGCAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 839 CCGCAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 5 ATCGAT AT^CGAT
BspMI 1201 ACCTGC ACCTGC(4/8)
BsrDI 147 CATTGC GCAATG(2/0)
BsrI 669 CCAGT ACTGG(1/-1)
BssSI 925 CACGAG CACGAG(-5/-1)
BstEII 411 GGTGACC G^GT[ACGT]ACC
BstPI 411 GGTGACC G^GT[ACGT]ACC
BstUI 421 CGCG CG^CG
ClaI 5 ATCGAT AT^CGAT
DraI 105 TTTAAA TTT^AAA
DsaI 680 CCATGG C^C[GA][CT]GG
EcoO65I 411 GGTGACC G^GT[ACGT]ACC
Hin1I 910 GACGCC G[GA]^CG[CT]C
KpnI 1131 GGTACC GGTAC^C
MvnI 421 CGCG CG^CG
NcoI 680 CCATGG C^CATGG
NspI 984 GCATGC [GA]CATG^[CT]
PmaCI 369 CACGTG CAC^GTG
PmlI 369 CACGTG CAC^GTG
SphI 984 GCATGC GCATG^C
XbaI 648 TCTAGA T^CTAGA
XcmI 226 CCACAGTTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 451 GTCTAC GT^[AC][GT]AC
856 GTCTAC
AfaI 1132 GTAC GT^AC
1144 GTAC
AflIII 368 ACACGT A^C[GA][CT]GT
370 ACGTGT
AvaII 495 GGTCC G^G[AT]CC
572 GGACC
BanI 394 GGCACC G^G[CT][GA]CC
1131 GGTACC
BcgI 702 CGAAGAGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
888 CGACTCCCCTGC
BciVI 64 GTATCC GTATCC(6/5)
343 GTATCC
BsgI 922 CTGCAC GTGCAG(16/14)
581 GTGCAG
BsmAI 440 GAGAC GTCTC(1/5)
1033 GAGAC
BstXI 485 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
996 CCAAGAGGGTGG
CfoI 420 GCGC GCG^C
618 GCGC
DpnII 4 GATC ^GATC
609 GATC
DrdI 442 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1035 GACAGAACTGTC
EcoT14I 338 CCAAGG C^C[AT][AT]GG
680 CCATGG
HgaI 910 GACGC GACGC(5/10)
422 GCGTC
HhaI 420 GCGC GCG^C
618 GCGC
HinP1I 420 GCGC G^CGC
618 GCGC
HinfI 889 GACTC G^A[ACGT]TC
904 GACTC
MboI 4 GATC ^GATC
609 GATC
MspA1I 318 CAGCGG C[AC]G^C[GT]G
899 CCGCTG
NdeII 4 GATC ^GATC
609 GATC
NlaIV 394 GGCACC GG[ACGT]^[ACGT]CC
1131 GGTACC
PleI 889 GACTC GAGTC(4/5)
904 GACTC
RsaI 1132 GTAC GT^AC
1144 GTAC
Sau3AI 4 GATC ^GATC
609 GATC
SfaNI 215 GCATC GCATC(5/9)
620 GCATC
StyI 338 CCAAGG C^C[AT][AT]GG
680 CCATGG
TaqI 6 TCGA T^CGA
701 TCGA
TspRI 186 CAGTG CA[GC]TG[ACGT][ACGT]^
1181 CAGTG
TthHB8I 6 TCGA T^CGA
701 TCGA
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BbvI BfaI BmyI
BpmI BsaJI BseRI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboII MnlI MseI
MslI MvaI MwoI NlaIII PstI
SapI Sau96I ScrFI SfcI Tru9I
TseI Tsp45I Tsp509I