Restriction map for
pF1KSDA0607
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AfaI AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbsI BciVI BglII
BlnI BpuAI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RsaI RsrII SacII SalI
ScaI SexAI SfuI SgrAI SnaBI
SspBI SspI SwaI Tsp509I Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1599 GACGTC GACGT^C
AflII 1015 CTTAAG C^TTAAG
Aor51HI 59 AGCGCT AGC^GCT
AvaII 372 GGACC G^G[AT]CC
BbeI 228 GGCGCC GGCGC^C
BbrPI 1768 CACGTG CAC^GTG
BbvCI 643 GCTGAGG CCTCAGC(-5/-2)
BfrI 1015 CTTAAG C^TTAAG
BlpI 765 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 765 GCTGAGC GC^T[ACGT]AGC
BsaAI 1768 CACGTG [CT]AC^GT[GA]
BsaBI 1386 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1165 GAGACC GGTCTC(1/5)
BsrBI 393 CCGCTC CCGCTC(-3/-3)
BsrDI 465 CATTGC GCAATG(2/0)
CelII 765 GCTGAGC GC^T[ACGT]AGC
Eco47III 59 AGCGCT AGC^GCT
KasI 228 GGCGCC G^GCGCC
MamI 1386 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1016 TTAA T^TAA
NarI 228 GGCGCC GG^CGCC
NheI 1806 GCTAGC G^CTAGC
NspI 1868 GCATGC [GA]CATG^[CT]
PmaCI 1768 CACGTG CAC^GTG
PmlI 1768 CACGTG CAC^GTG
PpuMI 371 GGGACCC [GA]G^G[AT]CC[CT]
SapI 1702 GCTCTTC GCTCTTC(1/4)
SbfI 1845 CCTGCAGG CCTGCA^GG
SfoI 228 GGCGCC GGC^GCC
SmlI 1015 CTTAAG C^T[CT][GA]AG
SpeI 1614 ACTAGT A^CTAGT
SphI 1868 GCATGC GCATG^C
Sse8387I 1845 CCTGCAGG CCTGCA^GG
StuI 1874 AGGCCT AGG^CCT
TaqI 179 TCGA T^CGA
Tru9I 1016 TTAA T^TAA
TthHB8I 179 TCGA T^CGA
XcmI 1303 CCAGGGCCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1775 CGCG CG^CG
2033 CGCG
BcgI 99 CGAGCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
698 CGACAACCGTGC
BclI 1391 TGATCA T^GATCA
1553 TGATCA
BsmI 715 GAATGC GAATGC(1/-1)
988 GAATGC
BspHI 1049 TCATGA T^CATGA
1595 TCATGA
BsrFI 1882 ACCGGC [GA]^CCGG[CT]
2042 ACCGGC
BssSI 1039 CTCGTG CACGAG(-5/-1)
1531 CTCGTG
BstAPI 1005 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1941 GCAGGAGCTGC
BstEII 948 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
BstPI 948 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
BstUI 1775 CGCG CG^CG
2033 CGCG
Cfr10I 1882 ACCGGC [GA]^CCGG[CT]
2042 ACCGGC
EarI 1703 CTCTTC CTCTTC(1/4)
1750 CTCTTC
EcoO65I 948 GGTGACC G^GT[ACGT]ACC
1536 GGTCACC
FbaI 1391 TGATCA T^GATCA
1553 TGATCA
HaeII 59 AGCGCT [GA]GCGC^[CT]
228 GGCGCC
HinfI 336 GATTC G^A[ACGT]TC
882 GAATC
Ksp632I 1703 CTCTTC CTCTTC(1/4)
1750 CTCTTC
MaeII 1600 ACGT A^CGT
1769 ACGT
MvnI 1775 CGCG CG^CG
2033 CGCG
PflMI 528 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGCAGGTGG
RcaI 1049 TCATGA T^CATGA
1595 TCATGA
TfiI 336 GATTC G^A[AT]TC
882 GAATC
Van91I 528 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1266 CCAGCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
ApaI AspHI AvaI BalI BamHI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfiI
SmaI StyI TseI Tsp45I TspRI
XhoII XmaCI XmaI