Restriction map for
pF1KSDA0604
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AclI AflII AgeI
AhdI Alw44I AlwI AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BbrPI BbvCI
BcgI BciVI BfrI BsaAI BsaBI
BsiWI BsmBI BspDI BspHI BsrBI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI DraI DraIII DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 703 GTCTAC GT^[AC][GT]AC
AflIII 1583 ACATGT A^C[GA][CT]GT
Aor51HI 524 AGCGCT AGC^GCT
ApaI 625 GGGCCC GGGCC^C
Asp700I 423 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 240 CCTAGG C^CTAGG
BalI 926 TGGCCA TGG^CCA
BbeI 1011 GGCGCC GGCGC^C
BclI 1385 TGATCA T^GATCA
BcnI 111 CCCGG CC^[GC]GG
BfaI 241 CTAG C^TAG
BglII 971 AGATCT A^GATCT
BlnI 240 CCTAGG C^CTAGG
BlpI 508 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 508 GCTGAGC GC^T[ACGT]AGC
BsiEI 1965 CGGCCG CG[GA][CT]^CG
Bsp1407I 1595 TGTACA T^GTACA
BspLU11I 1583 ACATGT A^CATGT
BsrFI 1355 ACCGGC [GA]^CCGG[CT]
BsrGI 1595 TGTACA T^GTACA
BstEII 2166 GGTGACC G^GT[ACGT]ACC
BstPI 2166 GGTGACC G^GT[ACGT]ACC
BstYI 971 AGATCT [GA]^GATC[CT]
CelII 508 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1355 ACCGGC [GA]^CCGG[CT]
EagI 1965 CGGCCG C^GGCCG
EclXI 1965 CGGCCG C^GGCCG
Eco47III 524 AGCGCT AGC^GCT
Eco52I 1965 CGGCCG C^GGCCG
EcoO65I 2166 GGTGACC G^GT[ACGT]ACC
FbaI 1385 TGATCA T^GATCA
HincII 1809 GTTGAC GT[CT]^[GA]AC
HindII 1809 GTTGAC GT[CT]^[GA]AC
KasI 1011 GGCGCC G^GCGCC
MaeI 241 CTAG C^TAG
MflI 971 AGATCT [GA]^GATC[CT]
MluNI 926 TGGCCA TGG^CCA
MscI 926 TGGCCA TGG^CCA
MseI 793 TTAA T^TAA
NarI 1011 GGCGCC GG^CGCC
NciI 111 CCCGG CC^[GC]GG
SapI 2097 GCTCTTC GCTCTTC(1/4)
SbfI 1731 CCTGCAGG CCTGCA^GG
SfoI 1011 GGCGCC GGC^GCC
SphI 1815 GCATGC GCATG^C
Sse8387I 1731 CCTGCAGG CCTGCA^GG
SspBI 1595 TGTACA T^GTACA
StuI 290 AGGCCT AGG^CCT
TaqI 300 TCGA T^CGA
Tru9I 793 TTAA T^TAA
TthHB8I 300 TCGA T^CGA
XcmI 363 CCAGTTCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 971 AGATCT [GA]^GATC[CT]
XmnI 423 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccIII 1397 TCCGGA T^CCGGA
1898 TCCGGA
AcsI 1556 AAATTT [GA]^AATT[CT]
1590 GAATTT
ApoI 1556 AAATTT [GA]^AATT[CT]
1590 GAATTT
AvaI 82 CCCGAG C^[CT]CG[GA]G
779 CTCGGG
BanI 193 GGTGCC G^G[CT][GA]CC
1011 GGCGCC
BsaWI 1397 TCCGGA [AT]^CCGG[AT]
1898 TCCGGA
BseAI 1397 TCCGGA T^CCGGA
1898 TCCGGA
BsgI 212 CTGCAC GTGCAG(16/14)
1220 CTGCAC
BsmI 1680 GAATGC GAATGC(1/-1)
296 GCATTC
BsoBI 82 CCCGAG C^[CT]CG[GA]G
779 CTCGGG
BspEI 1397 TCCGGA T^CCGGA
1898 TCCGGA
BsrDI 1989 CATTGC GCAATG(2/0)
737 GCAATG
BssSI 882 CACGAG CACGAG(-5/-1)
2155 CACGAG
BstAPI 897 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2061 GCAGCAACTGC
CpoI 1399 CGGACCG CG^G[AT]CCG
2132 CGGTCCG
EaeI 926 TGGCCA [CT]^GGCC[GA]
1965 CGGCCG
EarI 1333 CTCTTC CTCTTC(1/4)
2098 CTCTTC
EcoNI 282 CCTTACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
878 CCTCCACGAGG
HaeII 524 AGCGCT [GA]GCGC^[CT]
1011 GGCGCC
Ksp632I 1333 CTCTTC CTCTTC(1/4)
2098 CTCTTC
MaeII 5 ACGT A^CGT
1348 ACGT
MroI 1397 TCCGGA T^CCGGA
1898 TCCGGA
NcoI 119 CCATGG C^CATGG
1019 CCATGG
NspI 1583 ACATGT [GA]CATG^[CT]
1815 GCATGC
PflMI 1019 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1971 CCAGACGCTGG
PpuMI 673 GGGACCT [GA]G^G[AT]CC[CT]
1327 GGGTCCC
RsrII 1399 CGGACCG CG^G[AT]CCG
2132 CGGTCCG
SacI 1123 GAGCTC GAGCT^C
2148 GAGCTC
SexAI 1175 ACCTGGT A^CC[AT]GGT
1940 ACCAGGT
SmlI 1030 CTTGAG C^T[CT][GA]AG
1194 CTCAAG
Van91I 1019 CCATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1971 CCAGACGCTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanII BbsI
BbvI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI