Restriction map for
pF1KSDA0582
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflIII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BbrPI BclI BglI BlnI BsaAI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoT22I FbaI FseI FspI HincII
HindII HpaI KpnI KspI MaeII
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI StuI SwaI TaqI TthHB8I
XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1000 GTAGAC GT^[AC][GT]AC
AflII 1123 CTTAAG C^TTAAG
AgeI 1793 ACCGGT A^CCGGT
AspHI 2251 GAGCAC G[AT]GC[AT]^C
AspI 976 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1382 GGCGCC G^G[CT][GA]CC
BbeI 1382 GGCGCC GGCGC^C
BcgI 1440 CGAGTATCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 179 GGATAC GTATCC(6/5)
BfrI 1123 CTTAAG C^TTAAG
BglII 900 AGATCT A^GATCT
BsaBI 2026 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1793 ACCGGT [AT]^CCGG[AT]
BsiHKAI 2251 GAGCAC G[AT]GC[AT]^C
BsmBI 792 GAGACG CGTCTC(1/5)
BssSI 647 CTCGTG CACGAG(-5/-1)
DraIII 293 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1337 CCTCGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1534 GATATC GAT^ATC
HaeII 1382 GGCGCC [GA]GCGC^[CT]
HgaI 794 GACGC GACGC(5/10)
HindIII 370 AAGCTT A^AGCTT
KasI 1382 GGCGCC G^GCGCC
MamI 2026 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2254 CACCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1382 GGCGCC GG^CGCC
NspI 2198 ACATGC [GA]CATG^[CT]
PflFI 976 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1793 ACCGGT A^CCGGT
PpuMI 726 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1903 GAAGAGC GCTCTTC(1/4)
SbfI 984 CCTGCAGG CCTGCA^GG
ScaI 815 AGTACT AGT^ACT
SfoI 1382 GGCGCC GGC^GCC
SmaI 931 CCCGGG CCC^GGG
Sse8387I 984 CCTGCAGG CCTGCA^GG
Tsp509I 2009 AATT ^AATT
Tth111I 976 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 226 TCTAGA T^CTAGA
XmaCI 931 CCCGGG C^CCGGG
XmaI 931 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AcyI 794 GACGCC G[GA]^CG[CT]C
1382 GGCGCC
AfaI 816 GTAC GT^AC
1144 GTAC
AocI 1251 CCTGAGG CC^T[ACGT]AGG
1651 CCTGAGG
ApaI 103 GGGCCC GGGCC^C
1105 GGGCCC
AvaI 931 CCCGGG C^[CT]CG[GA]G
1738 CTCGGG
BalI 662 TGGCCA TGG^CCA
1798 TGGCCA
BamHI 1498 GGATCC G^GATCC
1647 GGATCC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
BsaHI 794 GACGCC G[GA]^CG[CT]C
1382 GGCGCC
BsiEI 689 CGGTCG CG[GA][CT]^CG
858 CGACCG
BsoBI 931 CCCGGG C^[CT]CG[GA]G
1738 CTCGGG
BspMI 183 ACCTGC ACCTGC(4/8)
1390 ACCTGC
BsrBI 1457 CCGCTC CCGCTC(-3/-3)
82 GAGCGG
BsrDI 1872 GCAATG GCAATG(2/0)
2245 GCAATG
BstXI 54 CCAGTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1635 CCAAGGATCTGG
Bsu36I 1251 CCTGAGG CC^T[ACGT]AGG
1651 CCTGAGG
CelII 552 GCTGAGC GC^T[ACGT]AGC
571 GCTCAGC
EaeI 662 TGGCCA [CT]^GGCC[GA]
1798 TGGCCA
Eco81I 1251 CCTGAGG CC^T[ACGT]AGG
1651 CCTGAGG
FauI 1456 CCCGC CCCGC(4/6)
84 GCGGG
Hin1I 794 GACGCC G[GA]^CG[CT]C
1382 GGCGCC
MluNI 662 TGGCCA TGG^CCA
1798 TGGCCA
MscI 662 TGGCCA TGG^CCA
1798 TGGCCA
MseI 1124 TTAA T^TAA
2012 TTAA
NcoI 317 CCATGG C^CATGG
2237 CCATGG
PflMI 483 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1096 CCATTTTCTGG
RsaI 816 GTAC GT^AC
1144 GTAC
SmlI 1123 CTTAAG C^T[CT][GA]AG
1981 CTCAAG
Tru9I 1124 TTAA T^TAA
2012 TTAA
Van91I 483 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1096 CCATTTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I TspRI XcmI XhoII