Restriction map for
pF1KSDA0576
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AlwNI
Aor51HI ApaI AscI Asp718I AspI
AviII AvrII BalI BbeI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AocI 49 CCTGAGG CC^T[ACGT]AGG
AseI 442 ATTAAT AT^TAAT
AsnI 442 ATTAAT AT^TAAT
AvaI 9 CCCGGG C^[CT]CG[GA]G
BamHI 1227 GGATCC G^GATCC
BanII 985 GAGCTC G[GA]GC[CT]^C
BbrPI 483 CACGTG CAC^GTG
BclI 1779 TGATCA T^GATCA
BsaAI 483 CACGTG [CT]AC^GT[GA]
BsaBI 1096 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2379 CTGCAC GTGCAG(16/14)
BsmFI 34 GTCCC GGGAC(10/14)
BsoBI 9 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2489 TGTACA T^GTACA
BspHI 2394 TCATGA T^CATGA
BspLU11I 1644 ACATGT A^CATGT
BsrBI 2776 CCGCTC CCGCTC(-3/-3)
BsrGI 2489 TGTACA T^GTACA
Bsu36I 49 CCTGAGG CC^T[ACGT]AGG
EaeI 2886 TGGCCG [CT]^GGCC[GA]
Eco81I 49 CCTGAGG CC^T[ACGT]AGG
EcoRI 1146 GAATTC G^AATTC
EcoT14I 2724 CCAAGG C^C[AT][AT]GG
EcoT22I 2878 ATGCAT ATGCA^T
FauI 257 CCCGC CCCGC(4/6)
FbaI 1779 TGATCA T^GATCA
MamI 1096 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2763 CAATTG C^AATTG
MunI 2763 CAATTG C^AATTG
NsiI 2878 ATGCAT ATGCA^T
PmaCI 483 CACGTG CAC^GTG
PmlI 483 CACGTG CAC^GTG
PshBI 442 ATTAAT AT^TAAT
RcaI 2394 TCATGA T^CATGA
SacI 985 GAGCTC GAGCT^C
SexAI 1411 ACCTGGT A^CC[AT]GGT
SmaI 9 CCCGGG CCC^GGG
SmlI 3172 CTTGAG C^T[CT][GA]AG
SspBI 2489 TGTACA T^GTACA
SspI 190 AATATT AAT^ATT
StyI 2724 CCAAGG C^C[AT][AT]GG
SwaI 1574 ATTTAAAT ATTT^AAAT
XbaI 2892 TCTAGA T^CTAGA
XmaCI 9 CCCGGG C^CCGGG
XmaI 9 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 258 CCGC CCGC(-3/-1)
2776 CCGC
AfaI 1953 GTAC GT^AC
2490 GTAC
AflIII 482 ACACGT A^C[GA][CT]GT
1644 ACATGT
AhdI 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1154 GACACAAAGTC
Alw44I 2323 GTGCAC G^TGCAC
2408 GTGCAC
ApaLI 2323 GTGCAC G^TGCAC
2408 GTGCAC
AspEI 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1154 GACACAAAGTC
BbsI 82 GAAGAC GAAGAC(2/6)
2976 GAAGAC
BpuAI 82 GAAGAC GAAGAC(2/6)
2976 GAAGAC
BsaI 5 GAGACC GGTCTC(1/5)
2143 GAGACC
BsmI 1423 GAATGC GAATGC(1/-1)
304 GCATTC
BspMI 2377 ACCTGC ACCTGC(4/8)
2642 ACCTGC
BstYI 1227 GGATCC [GA]^GATC[CT]
2470 GGATCT
DraI 1350 TTTAAA TTT^AAA
1575 TTTAAA
Eam1105I 7 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1154 GACACAAAGTC
HpaI 1617 GTTAAC GTT^AAC
2157 GTTAAC
MaeII 198 ACGT A^CGT
484 ACGT
MflI 1227 GGATCC [GA]^GATC[CT]
2470 GGATCT
MspA1I 41 CAGCTG C[AC]G^C[GT]G
1908 CAGCTG
NdeI 811 CATATG CA^TATG
2871 CATATG
PstI 1920 CTGCAG CTGCA^G
2262 CTGCAG
PvuII 41 CAGCTG CAG^CTG
1908 CAGCTG
RsaI 1953 GTAC GT^AC
2490 GTAC
SapI 160 GAAGAGC GCTCTTC(1/4)
1044 GAAGAGC
SphI 330 GCATGC GCATG^C
2880 GCATGC
Tsp45I 1648 GTGAC ^GT[GC]AC
3074 GTGAC
XcmI 2154 CCAGTTAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3131 CCACAGAAGATGTGG
XhoII 1227 GGATCC [GA]^GATC[CT]
2470 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AcsI AluI AlwI ApoI Asp700I
AspHI AvaII BanI BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XmnI