Restriction map for
pF1KSDA0552
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI AlwI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BciVI
BclI BfrI BlnI BpuAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 166 GGTACC G^GTACC
Alw44I 953 GTGCAC G^TGCAC
AocI 559 CCTGAGG CC^T[ACGT]AGG
ApaLI 953 GTGCAC G^TGCAC
Asp718I 166 GGTACC G^GTACC
BcgI 1530 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1343 AGATCT A^GATCT
BsaAI 1867 TACGTG [CT]AC^GT[GA]
BsaI 274 GGTCTC GGTCTC(1/5)
BsmBI 13 GAGACG CGTCTC(1/5)
BsrFI 1554 GCCGGC [GA]^CCGG[CT]
BstYI 1343 AGATCT [GA]^GATC[CT]
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1554 GCCGGC [GA]^CCGG[CT]
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
KpnI 166 GGTACC GGTAC^C
MflI 1343 AGATCT [GA]^GATC[CT]
MseI 743 TTAA T^TAA
MslI 322 CACACCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1554 GCCGGC GCC^GGC
NcoI 107 CCATGG C^CATGG
NgoMIV 1554 GCCGGC G^CCGGC
PaeR7I 1993 CTCGAG C^TCGAG
PshAI 268 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 1769 CCCGGG CCC^GGG
SpeI 471 ACTAGT A^CTAGT
Tru9I 743 TTAA T^TAA
XhoI 1993 CTCGAG C^TCGAG
XhoII 1343 AGATCT [GA]^GATC[CT]
XmaCI 1769 CCCGGG C^CCGGG
XmaI 1769 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 510 GAATTT [GA]^AATT[CT]
528 GAATTT
ApoI 510 GAATTT [GA]^AATT[CT]
528 GAATTT
AspHI 459 GTGCTC G[AT]GC[AT]^C
953 GTGCAC
BfaI 451 CTAG C^TAG
472 CTAG
BsiEI 478 CGGCCG CG[GA][CT]^CG
1577 CGGCCG
BsiHKAI 459 GTGCTC G[AT]GC[AT]^C
953 GTGCAC
BssHII 27 GCGCGC G^CGCGC
1149 GCGCGC
BstAPI 1173 GCAGGTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1927 GCAGGGGGTGC
BstXI 425 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1329 CCAGAAGGCTGG
EagI 478 CGGCCG C^GGCCG
1577 CGGCCG
EarI 156 GAAGAG CTCTTC(1/4)
1243 GAAGAG
EclXI 478 CGGCCG C^GGCCG
1577 CGGCCG
Eco52I 478 CGGCCG C^GGCCG
1577 CGGCCG
EcoT14I 107 CCATGG C^C[AT][AT]GG
1661 CCTTGG
HgaI 15 GACGC GACGC(5/10)
1707 GACGC
Ksp632I 156 GAAGAG CTCTTC(1/4)
1243 GAAGAG
MaeI 451 CTAG C^TAG
472 CTAG
MaeIII 317 GTGAC ^GT[ACGT]AC
383 GTGAC
SapI 156 GAAGAGC GCTCTTC(1/4)
1243 GAAGAGC
SfiI 574 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1638 GGCCGGGGTGGCC
SmlI 1549 CTCAAG C^T[CT][GA]AG
1993 CTCGAG
StuI 915 AGGCCT AGG^CCT
1973 AGGCCT
StyI 107 CCATGG C^C[AT][AT]GG
1661 CCTTGG
TaqI 1838 TCGA T^CGA
1994 TCGA
Tsp45I 317 GTGAC ^GT[GC]AC
383 GTGAC
Tsp509I 511 AATT ^AATT
529 AATT
TthHB8I 1838 TCGA T^CGA
1994 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AvaI AvaII BalI BanI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI TspRI Van91I
XcmI