Restriction map for
pF1KSDA0535
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbvCI BcgI BclI BglI
BlnI BsiEI BsiWI BsmBI BspDI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FbaI FseI
FspI HgaI HpaI KpnI KspI
MluI MvnI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 2368 TCCGGA T^CCGGA
AcyI 1645 GGCGCC G[GA]^CG[CT]C
AflIII 1131 ACACGT A^C[GA][CT]GT
AlwI 1107 GATCC GGATC(4/5)
Asp700I 304 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2325 CTCGGG C^[CT]CG[GA]G
AvaII 3004 GGACC G^G[AT]CC
BbeI 1645 GGCGCC GGCGC^C
BciVI 3131 GTATCC GTATCC(6/5)
BglII 2145 AGATCT A^GATCT
BlpI 940 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 940 GCTGAGC GC^T[ACGT]AGC
BsaBI 1345 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1645 GGCGCC G[GA]^CG[CT]C
BsaI 1093 GAGACC GGTCTC(1/5)
BsaWI 2368 TCCGGA [AT]^CCGG[AT]
BseAI 2368 TCCGGA T^CCGGA
BsoBI 2325 CTCGGG C^[CT]CG[GA]G
Bsp1407I 3056 TGTACA T^GTACA
BspEI 2368 TCCGGA T^CCGGA
BspMI 2947 GCAGGT ACCTGC(4/8)
BsrGI 3056 TGTACA T^GTACA
BssSI 2378 CTCGTG CACGAG(-5/-1)
BstXI 1656 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2145 AGATCT [GA]^GATC[CT]
CelII 940 GCTGAGC GC^T[ACGT]AGC
CfoI 1646 GCGC GCG^C
DraII 3003 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1180 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 104 CCATGG C^C[GA][CT]GG
EcoO109I 3003 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1423 GAATTC G^AATTC
HaeII 1645 GGCGCC [GA]GCGC^[CT]
HhaI 1646 GCGC GCG^C
Hin1I 1645 GGCGCC G[GA]^CG[CT]C
HinP1I 1646 GCGC G^CGC
HincII 151 GTCAAC GT[CT]^[GA]AC
HindII 151 GTCAAC GT[CT]^[GA]AC
KasI 1645 GGCGCC G^GCGCC
MamI 1345 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2145 AGATCT [GA]^GATC[CT]
MroI 2368 TCCGGA T^CCGGA
NarI 1645 GGCGCC GG^CGCC
NcoI 104 CCATGG C^CATGG
NdeI 3032 CATATG CA^TATG
NspI 1797 GCATGC [GA]CATG^[CT]
PpuMI 3003 GGGACCT [GA]G^G[AT]CC[CT]
SfoI 1645 GGCGCC GGC^GCC
SphI 1797 GCATGC GCATG^C
SspBI 3056 TGTACA T^GTACA
SwaI 443 ATTTAAAT ATTT^AAAT
XbaI 2070 TCTAGA T^CTAGA
XhoII 2145 AGATCT [GA]^GATC[CT]
XmnI 304 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflII 1845 CTTAAG C^TTAAG
2296 CTTAAG
BalI 2196 TGGCCA TGG^CCA
2466 TGGCCA
BcnI 1243 CCGGG CC^[GC]GG
3084 CCCGG
BfrI 1845 CTTAAG C^TTAAG
2296 CTTAAG
BspHI 284 TCATGA T^CATGA
2726 TCATGA
BstAPI 1717 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1720 GCAGCTGCTGC
DraI 444 TTTAAA TTT^AAA
468 TTTAAA
EcoT22I 1213 ATGCAT ATGCA^T
2216 ATGCAT
MfeI 926 CAATTG C^AATTG
1310 CAATTG
MluNI 2196 TGGCCA TGG^CCA
2466 TGGCCA
MscI 2196 TGGCCA TGG^CCA
2466 TGGCCA
MunI 926 CAATTG C^AATTG
1310 CAATTG
NciI 1243 CCGGG CC^[GC]GG
3084 CCCGG
NsiI 1213 ATGCAT ATGCA^T
2216 ATGCAT
RcaI 284 TCATGA T^CATGA
2726 TCATGA
StuI 1078 AGGCCT AGG^CCT
2105 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BanI BanII BbrPI
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EaeI EarI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PmaCI PmlI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I