Restriction map for
pF1KSDA0532
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII AccII AclI AflII AgeI
Aor51HI AscI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BfrI BlnI BsiEI BsiWI BsmI
BspDI BsrBI BssHII BssSI BstBI
BstEII BstPI BstUI ClaI CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FauI FseI
FspI HincII HindII HpaI KasI
KspI MluI MvnI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 3574 GGTACC G^GTACC
AccIII 2358 TCCGGA T^CCGGA
AcyI 3529 GGCGTC G[GA]^CG[CT]C
AflIII 47 ACATGT A^C[GA][CT]GT
AseI 3010 ATTAAT AT^TAAT
AsnI 3010 ATTAAT AT^TAAT
Asp718I 3574 GGTACC G^GTACC
BalI 387 TGGCCA TGG^CCA
BciVI 396 GGATAC GTATCC(6/5)
BclI 3765 TGATCA T^GATCA
BglI 3373 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2792 AGATCT A^GATCT
BsaAI 3049 TACGTG [CT]AC^GT[GA]
BsaBI 627 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3529 GGCGTC G[GA]^CG[CT]C
BsaI 3410 GGTCTC GGTCTC(1/5)
BsaWI 2358 TCCGGA [AT]^CCGG[AT]
BseAI 2358 TCCGGA T^CCGGA
BsmBI 3531 CGTCTC CGTCTC(1/5)
BspEI 2358 TCCGGA T^CCGGA
BspLU11I 47 ACATGT A^CATGT
BsrDI 3361 GCAATG GCAATG(2/0)
BstAPI 3342 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 2614 TTTAAA TTT^AAA
EaeI 387 TGGCCA [CT]^GGCC[GA]
EcoRI 1258 GAATTC G^AATTC
EcoT22I 1982 ATGCAT ATGCA^T
FbaI 3765 TGATCA T^GATCA
HgaI 3530 GCGTC GACGC(5/10)
Hin1I 3529 GGCGTC G[GA]^CG[CT]C
KpnI 3574 GGTACC GGTAC^C
MamI 627 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3749 CAATTG C^AATTG
MluNI 387 TGGCCA TGG^CCA
MroI 2358 TCCGGA T^CCGGA
MscI 387 TGGCCA TGG^CCA
MunI 3749 CAATTG C^AATTG
NsiI 1982 ATGCAT ATGCA^T
PaeR7I 2213 CTCGAG C^TCGAG
PflMI 4434 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 3010 ATTAAT AT^TAAT
ScaI 1974 AGTACT AGT^ACT
SexAI 4439 ACCTGGT A^CC[AT]GGT
StuI 4175 AGGCCT AGG^CCT
Van91I 4434 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2213 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccI 1751 GTATAC GT^[AC][GT]AC
3067 GTATAC
AhdI 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4341 GACATCTTGTC
AocI 319 CCTGAGG CC^T[ACGT]AGG
4503 CCTGAGG
AspEI 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4341 GACATCTTGTC
BanI 891 GGTGCC G^G[CT][GA]CC
3574 GGTACC
BlpI 2680 GCTGAGC GC^T[ACGT]AGC
3895 GCTGAGC
Bpu1102I 2680 GCTGAGC GC^T[ACGT]AGC
3895 GCTGAGC
BspHI 998 TCATGA T^CATGA
2328 TCATGA
BsrFI 3626 ACCGGC [GA]^CCGG[CT]
3656 ACCGGC
Bst1107I 1751 GTATAC GTA^TAC
3067 GTATAC
BstXI 3182 CCAGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3602 CCACAAGCCTGG
BstZ17I 1751 GTATAC GTA^TAC
3067 GTATAC
Bsu36I 319 CCTGAGG CC^T[ACGT]AGG
4503 CCTGAGG
CelII 2680 GCTGAGC GC^T[ACGT]AGC
3895 GCTGAGC
Cfr10I 3626 ACCGGC [GA]^CCGG[CT]
3656 ACCGGC
DsaI 1736 CCATGG C^C[GA][CT]GG
4484 CCATGG
Eam1105I 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4341 GACATCTTGTC
EarI 4138 CTCTTC CTCTTC(1/4)
2752 GAAGAG
Eco81I 319 CCTGAGG CC^T[ACGT]AGG
4503 CCTGAGG
EcoNI 2023 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3693 CCTGGGCGAGG
HindIII 296 AAGCTT A^AGCTT
1526 AAGCTT
Ksp632I 4138 CTCTTC CTCTTC(1/4)
2752 GAAGAG
MaeII 3050 ACGT A^CGT
4073 ACGT
NcoI 1736 CCATGG C^CATGG
4484 CCATGG
NdeI 1290 CATATG CA^TATG
1382 CATATG
PshAI 1960 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2203 GACCCTAGTC
PstI 2809 CTGCAG CTGCA^G
4400 CTGCAG
RcaI 998 TCATGA T^CATGA
2328 TCATGA
SapI 2752 GAAGAGC GCTCTTC(1/4)
4137 GCTCTTC
SmaI 2727 CCCGGG CCC^GGG
3503 CCCGGG
SspI 409 AATATT AAT^ATT
1809 AATATT
XbaI 123 TCTAGA T^CTAGA
2412 TCTAGA
XmaCI 2727 CCCGGG C^CCGGG
3503 CCCGGG
XmaI 2727 CCCGGG C^CCGGG
3503 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmnI