Restriction map for
pF1KSDA0529
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccI AclI AcyI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BanII BbeI BcgI BcnI
BlnI BlpI Bpu1102I BsaHI BsgI
BsiEI BsiWI BsmBI BsmI BspDI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KspI MluI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NsiI NspI NspV
PflFI PinAI PleI PmeI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 74 enzymes) #
###################################
Acc65I 89 GGTACC G^GTACC
AccII 48 CGCG CG^CG
AccIII 71 TCCGGA T^CCGGA
AflII 235 CTTAAG C^TTAAG
AflIII 802 ACGTGT A^C[GA][CT]GT
AocI 905 CCTTAGG CC^T[ACGT]AGG
Asp700I 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 89 GGTACC G^GTACC
AspHI 1600 GAGCAC G[AT]GC[AT]^C
AvaI 1644 CTCGAG C^[CT]CG[GA]G
BalI 658 TGGCCA TGG^CCA
BanI 89 GGTACC G^G[CT][GA]CC
BbrPI 1603 CACGTG CAC^GTG
BbvCI 2212 GCTGAGG CCTCAGC(-5/-2)
BclI 947 TGATCA T^GATCA
BfrI 235 CTTAAG C^TTAAG
BmyI 1600 GAGCAC G[AGT]GC[ACT]^C
BpmI 2396 CTCCAG CTGGAG(16/14)
BsaI 526 GAGACC GGTCTC(1/5)
BsaWI 71 TCCGGA [AT]^CCGG[AT]
BseAI 71 TCCGGA T^CCGGA
BsiHKAI 1600 GAGCAC G[AT]GC[AT]^C
BsoBI 1644 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1600 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1429 TGTACA T^GTACA
BspEI 71 TCCGGA T^CCGGA
BspHI 809 TCATGA T^CATGA
BsrBI 14 GAGCGG CCGCTC(-3/-3)
BsrGI 1429 TGTACA T^GTACA
BstAPI 1435 GCATTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 48 CGCG CG^CG
BstXI 1656 CCACCATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 905 CCTTAGG CC^T[ACGT]AGG
Cac8I 2361 GCAAGC GC[ACGT]^[ACGT]GC
DraI 1191 TTTAAA TTT^AAA
DraIII 1868 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2638 GACAGTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 658 TGGCCA [CT]^GGCC[GA]
EarI 2695 CTCTTC CTCTTC(1/4)
Eco81I 905 CCTTAGG CC^T[ACGT]AGG
EcoRI 2500 GAATTC G^AATTC
FbaI 947 TGATCA T^GATCA
HapII 72 CCGG C^CGG
HgaI 51 GACGC GACGC(5/10)
HincII 2018 GTCAAC GT[CT]^[GA]AC
HindII 2018 GTCAAC GT[CT]^[GA]AC
HpaII 72 CCGG C^CGG
KpnI 89 GGTACC GGTAC^C
Ksp632I 2695 CTCTTC CTCTTC(1/4)
MfeI 479 CAATTG C^AATTG
MluNI 658 TGGCCA TGG^CCA
MroI 71 TCCGGA T^CCGGA
MscI 658 TGGCCA TGG^CCA
MspI 72 CCGG C^CGG
MunI 479 CAATTG C^AATTG
MvnI 48 CGCG CG^CG
NdeI 2687 CATATG CA^TATG
NruI 47 TCGCGA TCG^CGA
PacI 2506 TTAATTAA TTAAT^TAA
PaeR7I 1644 CTCGAG C^TCGAG
PflMI 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1603 CACGTG CAC^GTG
PmlI 1603 CACGTG CAC^GTG
PstI 1460 CTGCAG CTGCA^G
RcaI 809 TCATGA T^CATGA
ScaI 2400 AGTACT AGT^ACT
SexAI 85 ACCTGGT A^CC[AT]GGT
SfcI 1460 CTGCAG C^T[GA][CT]AG
SfiI 772 GGCCTCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1429 TGTACA T^GTACA
StuI 771 AGGCCT AGG^CCT
Van91I 530 CCAGATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1644 CTCGAG C^TCGAG
XmnI 1199 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 1828 ATTAAT AT^TAAT
2509 ATTAAT
AsnI 1828 ATTAAT AT^TAAT
2509 ATTAAT
BglI 773 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1841 GCCCAAATGGC
BglII 1422 AGATCT A^GATCT
2149 AGATCT
BsaAI 801 TACGTG [CT]AC^GT[GA]
1603 CACGTG
BsaBI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
BseRI 1967 GAGGAG GAGGAG(10/8)
2576 GAGGAG
BspMI 1458 ACCTGC ACCTGC(4/8)
2521 GCAGGT
BsrDI 1313 CATTGC GCAATG(2/0)
2807 GCAATG
DraII 666 GGGTCCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
EcoO109I 666 GGGTCCT [GA]G^G[ACGT]CC[CT]
2523 AGGTCCT
EcoRV 1037 GATATC GAT^ATC
2080 GATATC
MamI 871 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2167 GATTGGGATC
MslI 312 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2230 CATGAAAGTG
PpuMI 666 GGGTCCT [GA]G^G[AT]CC[CT]
2523 AGGTCCT
PshBI 1828 ATTAAT AT^TAAT
2509 ATTAAT
PvuII 303 CAGCTG CAG^CTG
2768 CAGCTG
SspI 1509 AATATT AAT^ATT
1825 AATATT
Tsp45I 231 GTCAC ^GT[GC]AC
982 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BamHI BbsI BbvI
BciVI BfaI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII