Restriction map for
pF1KSDA0526
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccII AccIII AflII AflIII
AgeI Alw44I AlwNI AocI ApaLI
AscI AseI AsnI AspI AviII
AvrII BalI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII ClaI CpoI DraIII
DrdI DsaI EarI Eco81I EcoNI
EcoO65I FbaI FseI FspI HincII
HindII HpaI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI MvnI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1632 GGTACC G^GTACC
AccI 483 GTATAC GT^[AC][GT]AC
AclI 1125 AACGTT AA^CGTT
AhdI 328 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 97 AGCGCT AGC^GCT
ApaI 1056 GGGCCC GGGCC^C
Asp700I 1196 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1632 GGTACC G^GTACC
AspEI 328 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 214 GTGCTC G[AT]GC[AT]^C
AvaI 1099 CCCGAG C^[CT]CG[GA]G
BamHI 1095 GGATCC G^GATCC
BglI 1058 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 4 CGGCCG CG[GA][CT]^CG
BsiHKAI 214 GTGCTC G[AT]GC[AT]^C
BsmFI 1595 GGGAC GGGAC(10/14)
BsoBI 1099 CCCGAG C^[CT]CG[GA]G
BspHI 459 TCATGA T^CATGA
BspMI 1190 ACCTGC ACCTGC(4/8)
BsrFI 124 GCCGGC [GA]^CCGG[CT]
Bst1107I 483 GTATAC GTA^TAC
BstZ17I 483 GTATAC GTA^TAC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraI 1572 TTTAAA TTT^AAA
EagI 4 CGGCCG C^GGCCG
Eam1105I 328 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4 CGGCCG C^GGCCG
Eco47III 97 AGCGCT AGC^GCT
Eco52I 4 CGGCCG C^GGCCG
EcoRI 723 GAATTC G^AATTC
EcoRV 254 GATATC GAT^ATC
EcoT14I 1299 CCTTGG C^C[AT][AT]GG
EcoT22I 788 ATGCAT ATGCA^T
FauI 42 GCGGG CCCGC(4/6)
HgaI 1352 GACGC GACGC(5/10)
HindIII 182 AAGCTT A^AGCTT
KpnI 1632 GGTACC GGTAC^C
MspA1I 1606 CAGCTG C[AC]G^C[GT]G
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
NsiI 788 ATGCAT ATGCA^T
NspI 1421 ACATGC [GA]CATG^[CT]
PpuMI 1595 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1125 AACGTT AA^CGTT
PstI 101 CTGCAG CTGCA^G
PvuII 1606 CAGCTG CAG^CTG
RcaI 459 TCATGA T^CATGA
SexAI 1336 ACCAGGT A^CC[AT]GGT
SfiI 1057 GGCCCCACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 1299 CCTTGG C^C[AT][AT]GG
TaqI 261 TCGA T^CGA
TthHB8I 261 TCGA T^CGA
XmnI 1196 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 723 GAATTC [GA]^AATT[CT]
926 AAATTC
ApoI 723 GAATTC [GA]^AATT[CT]
926 AAATTC
AvaII 473 GGTCC G^G[AT]CC
1596 GGACC
BanII 10 GAGCCC G[GA]GC[CT]^C
1056 GGGCCC
BbeI 1048 GGCGCC GGCGC^C
1438 GGCGCC
BcnI 13 CCCGG CC^[GC]GG
1068 CCGGG
BfaI 655 CTAG C^TAG
862 CTAG
BsaAI 85 TACGTG [CT]AC^GT[GA]
219 CACGTA
BsgI 609 GTGCAG GTGCAG(16/14)
1211 GTGCAG
BsmBI 1350 GAGACG CGTCTC(1/5)
1660 GAGACG
Bsp1407I 377 TGTACA T^GTACA
1314 TGTACA
BsrGI 377 TGTACA T^GTACA
1314 TGTACA
BstAPI 752 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1213 GCAGTGTATGC
DdeI 678 CTTAG C^T[ACGT]AG
769 CTGAG
KasI 1048 GGCGCC G^GCGCC
1438 GGCGCC
MaeI 655 CTAG C^TAG
862 CTAG
MseI 176 TTAA T^TAA
1573 TTAA
NarI 1048 GGCGCC GG^CGCC
1438 GGCGCC
NciI 13 CCCGG CC^[GC]GG
1068 CCGGG
PleI 1393 GACTC GAGTC(4/5)
1513 GAGTC
ScaI 613 AGTACT AGT^ACT
1082 AGTACT
SfoI 1048 GGCGCC GGC^GCC
1438 GGCGCC
SspBI 377 TGTACA T^GTACA
1314 TGTACA
Tru9I 176 TTAA T^TAA
1573 TTAA
Tsp45I 296 GTCAC ^GT[GC]AC
1233 GTCAC
TspRI 794 CACTG CA[GC]TG[ACGT][ACGT]^
1214 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
BanI BbsI BbvI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DpnII DraII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI TseI
Tsp509I XhoII