Restriction map for
pF1KSDA0479
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BspDI BspEI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MunI
MvnI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AcsI 225 GAATTC [GA]^AATT[CT]
AfaI 903 GTAC GT^AC
ApoI 225 GAATTC [GA]^AATT[CT]
AvaI 692 CCCGGG C^[CT]CG[GA]G
BalI 428 TGGCCA TGG^CCA
BamHI 581 GGATCC G^GATCC
BanII 631 GGGCTC G[GA]GC[CT]^C
BbvCI 477 CCTCAGC CCTCAGC(-5/-2)
BglII 452 AGATCT A^GATCT
BpmI 610 CTGGAG CTGGAG(16/14)
BseRI 729 CTCCTC GAGGAG(10/8)
BsgI 103 CTGCAC GTGCAG(16/14)
BsmBI 196 CGTCTC CGTCTC(1/5)
BsmI 534 GAATGC GAATGC(1/-1)
BsoBI 692 CCCGGG C^[CT]CG[GA]G
Bsp1407I 902 TGTACA T^GTACA
BsrGI 902 TGTACA T^GTACA
BssSI 178 CTCGTG CACGAG(-5/-1)
DdeI 478 CTCAG C^T[ACGT]AG
EcoRI 225 GAATTC G^AATTC
EcoT14I 250 CCTTGG C^C[AT][AT]GG
HincII 349 GTCAAC GT[CT]^[GA]AC
HindII 349 GTCAAC GT[CT]^[GA]AC
MluNI 428 TGGCCA TGG^CCA
MscI 428 TGGCCA TGG^CCA
NaeI 188 GCCGGC GCC^GGC
NgoMIV 188 GCCGGC G^CCGGC
PflMI 846 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 308 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 903 GTAC GT^AC
SgrAI 503 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 692 CCCGGG CCC^GGG
SspBI 902 TGTACA T^GTACA
StyI 250 CCTTGG C^C[AT][AT]GG
TaqI 878 TCGA T^CGA
Tsp509I 226 AATT ^AATT
TspRI 441 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 878 TCGA T^CGA
Van91I 846 CCATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 692 CCCGGG C^CCGGG
XmaI 692 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 246 GGACC G^G[AT]CC
309 GGACC
BanI 191 GGCACC G^G[CT][GA]CC
687 GGTGCC
BsaI 7 GAGACC GGTCTC(1/5)
394 GAGACC
BspHI 314 TCATGA T^CATGA
719 TCATGA
BspMI 283 ACCTGC ACCTGC(4/8)
605 ACCTGC
BsrI 112 ACTGG ACTGG(1/-1)
328 ACTGG
BstXI 449 CCAAGATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
628 CCAGGGCTCTGG
DraII 173 AGGGCCT [GA]G^G[ACGT]CC[CT]
308 GGGACCT
EarI 318 GAAGAG CTCTTC(1/4)
565 GAAGAG
EcoO109I 173 AGGGCCT [GA]G^G[ACGT]CC[CT]
308 GGGACCT
FauI 501 CCCGC CCCGC(4/6)
134 GCGGG
Ksp632I 318 GAAGAG CTCTTC(1/4)
565 GAAGAG
MaeII 23 ACGT A^CGT
425 ACGT
MboII 318 GAAGA GAAGA(8/7)
565 GAAGA
MspA1I 211 CAGCTG C[AC]G^C[GT]G
898 CAGCTG
PleI 157 GACTC GAGTC(4/5)
613 GAGTC
PvuII 211 CAGCTG CAG^CTG
898 CAGCTG
RcaI 314 TCATGA T^CATGA
719 TCATGA
TfiI 716 GAATC G^A[AT]TC
723 GAATC
XcmI 153 CCACGACTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
812 CCAAGAGCAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI BstYI Cac8I Cfr10I Cfr13I
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MflI MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I XhoII