Restriction map for
pF1KSDA0436
##################################
# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AccIII AcyI AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BcnI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HincII
HindII HpaI HpaII KasI KspI
MamI MfeI MluI MroI MspI
MunI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 1674 GGTACC G^GTACC
AclI 1595 AACGTT AA^CGTT
AlwNI 713 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 1674 GGTACC G^GTACC
BalI 1362 TGGCCA TGG^CCA
BanII 582 GAGCCC G[GA]GC[CT]^C
BbsI 1033 GAAGAC GAAGAC(2/6)
BbvCI 1624 CCTCAGC CCTCAGC(-5/-2)
BbvI 244 GCTGC GCAGC(8/12)
BciVI 918 GGATAC GTATCC(6/5)
BglI 1206 GCCTGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 297 GCTCAGC GC^T[ACGT]AGC
BmyI 582 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 297 GCTCAGC GC^T[ACGT]AGC
BpuAI 1033 GAAGAC GAAGAC(2/6)
BsaI 878 GGTCTC GGTCTC(1/5)
BsmAI 879 GTCTC GTCTC(1/5)
Bsp1286I 582 GAGCCC G[AGT]GC[ACT]^C
BspHI 414 TCATGA T^CATGA
BsrBI 1463 GAGCGG CCGCTC(-3/-3)
BstYI 1890 GGATCT [GA]^GATC[CT]
CelII 297 GCTCAGC GC^T[ACGT]AGC
EarI 762 GAAGAG CTCTTC(1/4)
EcoT22I 5 ATGCAT ATGCA^T
FauI 1215 GCGGG CCCGC(4/6)
HgaI 1353 GACGC GACGC(5/10)
HindIII 320 AAGCTT A^AGCTT
KpnI 1674 GGTACC GGTAC^C
Ksp632I 762 GAAGAG CTCTTC(1/4)
MflI 1890 GGATCT [GA]^GATC[CT]
MluNI 1362 TGGCCA TGG^CCA
MscI 1362 TGGCCA TGG^CCA
MspA1I 713 CAGCGG C[AC]G^C[GT]G
NdeI 1655 CATATG CA^TATG
NsiI 5 ATGCAT ATGCA^T
PleI 1126 GACTC GAGTC(4/5)
Psp1406I 1595 AACGTT AA^CGTT
RcaI 414 TCATGA T^CATGA
ScaI 600 AGTACT AGT^ACT
SfcI 689 CTACAG C^T[GA][CT]AG
SpeI 1051 ACTAGT A^CTAGT
SphI 1731 GCATGC GCATG^C
TseI 244 GCTGC G^C[AT]GC
Tsp45I 1468 GTGAC ^GT[GC]AC
XcmI 1254 CCATGTTCGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1890 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 1057 CGCG CG^CG
1725 CGCG
AflII 184 CTTAAG C^TTAAG
1894 CTTAAG
AflIII 791 ACATGT A^C[GA][CT]GT
1161 ACATGT
AlwI 801 GGATC GGATC(4/5)
1890 GGATC
BanI 1479 GGCACC G^G[CT][GA]CC
1674 GGTACC
BfrI 184 CTTAAG C^TTAAG
1894 CTTAAG
BspLU11I 791 ACATGT A^CATGT
1161 ACATGT
BssKI 1221 CCTGG ^CC[ACGT]GG
1789 CCTGG
BstNI 1221 CCTGG CC^[AT]GG
1789 CCTGG
BstUI 1057 CGCG CG^CG
1725 CGCG
EaeI 1299 TGGCCG [CT]^GGCC[GA]
1362 TGGCCA
EcoRII 1221 CCTGG ^CC[AT]GG
1789 CCTGG
MaeIII 1468 GTGAC ^GT[ACGT]AC
1598 GTTAC
MvaI 1221 CCTGG CC^[AT]GG
1789 CCTGG
MvnI 1057 CGCG CG^CG
1725 CGCG
ScrFI 1221 CCTGG CC^[ACGT]GG
1789 CCTGG
SfaNI 4 GATGC GCATC(5/9)
274 GCATC
StuI 1204 AGGCCT AGG^CCT
1278 AGGCCT
XbaI 1068 TCTAGA T^CTAGA
1776 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BfaI BpmI BsaJI BsiYI
BslI BsmFI BsrI BstF5I Cac8I
Cfr13I DdeI DpnII DraII EcoNI
EcoO109I EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeII
MboI MboII MnlI MseI MslI
MwoI NdeII NlaIII NlaIV NspI
PpuMI RsaI Sau3AI Sau96I SmlI
SspI StyI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I