Restriction map for
pF1KSDA0427
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 1343 TCCGGA T^CCGGA
AcyI 1743 GACGCC G[GA]^CG[CT]C
AlwI 1131 GATCC GGATC(4/5)
BalI 1640 TGGCCA TGG^CCA
BfaI 1702 CTAG C^TAG
BglI 910 GCCCCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 689 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 689 GCTCAGC GC^T[ACGT]AGC
BsaAI 91 TACGTG [CT]AC^GT[GA]
BsaBI 1125 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1743 GACGCC G[GA]^CG[CT]C
BsaWI 1343 TCCGGA [AT]^CCGG[AT]
BseAI 1343 TCCGGA T^CCGGA
BsgI 109 GTGCAG GTGCAG(16/14)
BsmBI 1087 GAGACG CGTCTC(1/5)
BspEI 1343 TCCGGA T^CCGGA
BsrDI 806 GCAATG GCAATG(2/0)
BssSI 1495 CTCGTG CACGAG(-5/-1)
BstEII 628 GGTGACC G^GT[ACGT]ACC
BstPI 628 GGTGACC G^GT[ACGT]ACC
BstYI 1130 AGATCC [GA]^GATC[CT]
CelII 689 GCTCAGC GC^T[ACGT]AGC
EcoO65I 628 GGTGACC G^GT[ACGT]ACC
FauI 890 CCCGC CCCGC(4/6)
HgaI 1743 GACGC GACGC(5/10)
Hin1I 1743 GACGCC G[GA]^CG[CT]C
MaeI 1702 CTAG C^TAG
MaeIII 629 GTGAC ^GT[ACGT]AC
MamI 1125 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1130 AGATCC [GA]^GATC[CT]
MluNI 1640 TGGCCA TGG^CCA
MroI 1343 TCCGGA T^CCGGA
MscI 1640 TGGCCA TGG^CCA
NaeI 1595 GCCGGC GCC^GGC
NgoMIV 1595 GCCGGC G^CCGGC
PaeR7I 880 CTCGAG C^TCGAG
PflMI 525 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1675 GAGTC GAGTC(4/5)
ScaI 1760 AGTACT AGT^ACT
SexAI 104 ACCAGGT A^CC[AT]GGT
SmlI 880 CTCGAG C^T[CT][GA]AG
SphI 1142 GCATGC GCATG^C
TfiI 316 GATTC G^A[AT]TC
Tsp45I 629 GTGAC ^GT[GC]AC
Tsp509I 1017 AATT ^AATT
Van91I 525 CCATGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 880 CTCGAG C^TCGAG
XhoII 1130 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 424 CGCG CG^CG
1387 CGCG
AocI 704 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
Aor51HI 74 AGCGCT AGC^GCT
1415 AGCGCT
Asp700I 552 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGGACTTC
AspI 159 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1686 GACCCGGTC
AviII 1241 TGCGCA TGC^GCA
1463 TGCGCA
BbsI 1032 GTCTTC GAAGAC(2/6)
1450 GTCTTC
BpuAI 1032 GTCTTC GAAGAC(2/6)
1450 GTCTTC
BsaI 594 GAGACC GGTCTC(1/5)
817 GAGACC
BspMI 341 ACCTGC ACCTGC(4/8)
1513 ACCTGC
BsrFI 740 ACCGGC [GA]^CCGG[CT]
1595 GCCGGC
BstAPI 111 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
402 GCAGCCCCTGC
BstUI 424 CGCG CG^CG
1387 CGCG
BstXI 618 CCAACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1181 CCACCTTCATGG
Bsu36I 704 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
Cfr10I 740 ACCGGC [GA]^CCGG[CT]
1595 GCCGGC
Eco47III 74 AGCGCT AGC^GCT
1415 AGCGCT
Eco81I 704 CCTCAGG CC^T[ACGT]AGG
1518 CCTCAGG
EcoT14I 723 CCATGG C^C[AT][AT]GG
753 CCATGG
FspI 1241 TGCGCA TGC^GCA
1463 TGCGCA
HinfI 316 GATTC G^A[ACGT]TC
1675 GAGTC
MslI 802 CACCGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1182 CACCTTCATG
MvnI 424 CGCG CG^CG
1387 CGCG
NcoI 723 CCATGG C^CATGG
753 CCATGG
PflFI 159 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1686 GACCCGGTC
StyI 723 CCATGG C^C[AT][AT]GG
753 CCATGG
Tth111I 159 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1686 GACCCGGTC
XmnI 552 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGGACTTC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I