Restriction map for
pF1KSDA0416
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BglI
BlnI BpuAI BsaAI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AfaI 520 GTAC GT^AC
AlwNI 1519 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 201 CCTGAGG CC^T[ACGT]AGG
ApaI 29 GGGCCC GGGCC^C
Asp700I 1225 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 19 TGGCCA TGG^CCA
BglII 884 AGATCT A^GATCT
BlpI 681 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 681 GCTAAGC GC^T[ACGT]AGC
BsaBI 277 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 919 GGTCTC GGTCTC(1/5)
BsmI 940 GAATGC GAATGC(1/-1)
BspMI 401 ACCTGC ACCTGC(4/8)
Bsu36I 201 CCTGAGG CC^T[ACGT]AGG
CelII 681 GCTAAGC GC^T[ACGT]AGC
CfoI 947 GCGC GCG^C
DraI 69 TTTAAA TTT^AAA
DraIII 157 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1062 CCATGG C^C[GA][CT]GG
EaeI 19 TGGCCA [CT]^GGCC[GA]
Eco81I 201 CCTGAGG CC^T[ACGT]AGG
FauI 1263 GCGGG CCCGC(4/6)
HaeII 946 AGCGCC [GA]GCGC^[CT]
HhaI 947 GCGC GCG^C
HinP1I 947 GCGC G^CGC
HphI 630 TCACC GGTGA(8/7)
MaeII 492 ACGT A^CGT
MamI 277 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 19 TGGCCA TGG^CCA
MscI 19 TGGCCA TGG^CCA
NcoI 1062 CCATGG C^CATGG
NdeI 1420 CATATG CA^TATG
PacI 610 TTAATTAA TTAAT^TAA
PpuMI 1476 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 520 GTAC GT^AC
SspI 360 AATATT AAT^ATT
TfiI 877 GATTC G^A[AT]TC
Tsp45I 1099 GTCAC ^GT[GC]AC
XbaI 1050 TCTAGA T^CTAGA
XmnI 1225 GAACACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 331 CTTAAG C^TTAAG
345 CTTAAG
AflIII 733 ACATGT A^C[GA][CT]GT
1430 ACATGT
BfrI 331 CTTAAG C^TTAAG
345 CTTAAG
BpmI 1329 CTCCAG CTGGAG(16/14)
547 CTGGAG
BsgI 442 CTGCAC GTGCAG(16/14)
691 CTGCAC
BsmAI 1178 GAGAC GTCTC(1/5)
920 GTCTC
BsmFI 533 GGGAC GGGAC(10/14)
198 GTCCC
BspLU11I 733 ACATGT A^CATGT
1430 ACATGT
BsrDI 55 GCAATG GCAATG(2/0)
593 GCAATG
BsrI 781 ACTGG ACTGG(1/-1)
517 CCAGT
BstAPI 46 GCAATATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
477 GCAGACCTTGC
BstF5I 1008 CATCC GGATG(2/0)
740 GGATG
FokI 1008 CATCC GGATG(9/13)
740 GGATG
HincII 254 GTCAAC GT[CT]^[GA]AC
1089 GTCAAC
HindII 254 GTCAAC GT[CT]^[GA]AC
1089 GTCAAC
MslI 104 CACCCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1101 CACTGTCATG
NspI 733 ACATGT [GA]CATG^[CT]
1430 ACATGT
PleI 142 GACTC GAGTC(4/5)
1415 GACTC
PstI 475 CTGCAG CTGCA^G
1199 CTGCAG
SacI 223 GAGCTC GAGCT^C
451 GAGCTC
SfcI 475 CTGCAG C^T[GA][CT]AG
1199 CTGCAG
SmlI 331 CTTAAG C^T[CT][GA]AG
345 CTTAAG
TaqI 227 TCGA T^CGA
803 TCGA
TthHB8I 227 TCGA T^CGA
803 TCGA
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AseI AsnI AspHI AvaII BanII
BbvI BfaI BmyI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BssKI BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII EcoT14I
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PshBI PvuII
SapI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp509I TspRI
XhoII