Restriction map for
pF1KSDA0410
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraII DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII HpaI HphI KasI
KpnI Ksp632I KspI MaeII MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NarI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflII 841 CTTAAG C^TTAAG
AlwI 205 GGATC GGATC(4/5)
BbvCI 386 CCTCAGC CCTCAGC(-5/-2)
BfrI 841 CTTAAG C^TTAAG
BsaBI 730 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 669 CTCCTC GAGGAG(10/8)
BspHI 1218 TCATGA T^CATGA
BsrFI 55 GCCGGC [GA]^CCGG[CT]
BstXI 41 CCACCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 205 GGATCT [GA]^GATC[CT]
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
DpnII 206 GATC ^GATC
DrdI 402 GACTCTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 50 TGGCCG [CT]^GGCC[GA]
EcoNI 1426 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 59 GCGGG CCCGC(4/6)
HgaI 100 GCGTC GACGC(5/10)
HincII 597 GTTGAC GT[CT]^[GA]AC
HindII 597 GTTGAC GT[CT]^[GA]AC
MaeIII 1342 GTTAC ^GT[ACGT]AC
MamI 730 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 206 GATC ^GATC
MflI 205 GGATCT [GA]^GATC[CT]
NaeI 55 GCCGGC GCC^GGC
NcoI 1394 CCATGG C^CATGG
NdeII 206 GATC ^GATC
NgoMIV 55 GCCGGC G^CCGGC
NheI 391 GCTAGC G^CTAGC
PflMI 1316 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1218 TCATGA T^CATGA
Sau3AI 206 GATC ^GATC
ScaI 145 AGTACT AGT^ACT
SgrAI 54 CGCCGGCG C[GA]^CCGG[CT]G
TspRI 219 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 1316 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 205 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AcsI 778 AAATTT [GA]^AATT[CT]
1172 AAATTT
ApoI 778 AAATTT [GA]^AATT[CT]
1172 AAATTT
BbsI 987 GAAGAC GAAGAC(2/6)
825 GTCTTC
BcnI 26 CCGGG CC^[GC]GG
194 CCGGG
BpuAI 987 GAAGAC GAAGAC(2/6)
825 GTCTTC
BspMI 213 ACCTGC ACCTGC(4/8)
342 ACCTGC
BsrDI 635 GCAATG GCAATG(2/0)
835 GCAATG
BsrI 220 ACTGG ACTGG(1/-1)
1348 ACTGG
BstNI 756 CCAGG CC^[AT]GG
997 CCTGG
DsaI 47 CCGTGG C^C[GA][CT]GG
1394 CCATGG
EcoRII 756 CCAGG ^CC[AT]GG
997 CCTGG
MvaI 756 CCAGG CC^[AT]GG
997 CCTGG
NciI 26 CCGGG CC^[GC]GG
194 CCGGG
PleI 30 GACTC GAGTC(4/5)
402 GACTC
StuI 501 AGGCCT AGG^CCT
642 AGGCCT
TfiI 332 GATTC G^A[AT]TC
1040 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwNI Asp700I
AvaII BanII BbvI BfaI BmyI
BpmI BsaJI BsiYI BslI BsmFI
BsmI Bsp1286I BssKI BstAPI BstF5I
Cac8I Cfr13I DdeI EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MaeI MboII MnlI MseI
MslI MspI MwoI NlaIII NlaIV
PstI RsaI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp509I XmnI