Restriction map for
pF1KSDA0375
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# NO CUT ENZYMES ( 77 enzymes) #
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AclI AcsI AflII AflIII AgeI
AhdI ApoI AscI AseI AsnI
AspEI AspI AviII BbrPI BcgI
BclI BfrI BglII BsaBI BsiEI
BsiWI BsmI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BssHII BstBI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI HgaI KspI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfuI SgrAI SnaBI
SphI SspBI SspI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 3812 GGTACC G^GTACC
AccII 3133 CGCG CG^CG
AccIII 2348 TCCGGA T^CCGGA
Alw44I 58 GTGCAC G^TGCAC
AocI 2237 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1481 AGCGCT AGC^GCT
ApaLI 58 GTGCAC G^TGCAC
Asp700I 3071 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3812 GGTACC G^GTACC
AvrII 174 CCTAGG C^CTAGG
BbeI 952 GGCGCC GGCGC^C
BbsI 3385 GAAGAC GAAGAC(2/6)
BciVI 540 GGATAC GTATCC(6/5)
BlnI 174 CCTAGG C^CTAGG
BpuAI 3385 GAAGAC GAAGAC(2/6)
BsaAI 4498 TACGTG [CT]AC^GT[GA]
BseAI 2348 TCCGGA T^CCGGA
BsmBI 3653 GAGACG CGTCTC(1/5)
BspDI 3844 ATCGAT AT^CGAT
BspEI 2348 TCCGGA T^CCGGA
BsrBI 3679 GAGCGG CCGCTC(-3/-3)
BssSI 3875 CTCGTG CACGAG(-5/-1)
Bst1107I 1438 GTATAC GTA^TAC
BstUI 3133 CGCG CG^CG
BstZ17I 1438 GTATAC GTA^TAC
Bsu36I 2237 CCTCAGG CC^T[ACGT]AGG
ClaI 3844 ATCGAT AT^CGAT
Eco47III 1481 AGCGCT AGC^GCT
Eco81I 2237 CCTCAGG CC^T[ACGT]AGG
EcoT22I 871 ATGCAT ATGCA^T
HincII 2998 GTTAAC GT[CT]^[GA]AC
HindII 2998 GTTAAC GT[CT]^[GA]AC
HindIII 2556 AAGCTT A^AGCTT
HpaI 2998 GTTAAC GTT^AAC
KasI 952 GGCGCC G^GCGCC
KpnI 3812 GGTACC GGTAC^C
MroI 2348 TCCGGA T^CCGGA
MvnI 3133 CGCG CG^CG
NarI 952 GGCGCC GG^CGCC
NsiI 871 ATGCAT ATGCA^T
NspI 3197 ACATGC [GA]CATG^[CT]
PshAI 1834 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4305 CCTGCAGG CCTGCA^GG
ScaI 3113 AGTACT AGT^ACT
SfiI 3637 GGCCAGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 952 GGCGCC GGC^GCC
SpeI 4254 ACTAGT A^CTAGT
Sse8387I 4305 CCTGCAGG CCTGCA^GG
TfiI 703 GAATC G^A[AT]TC
XmnI 3071 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AatII 1475 GACGTC GACGT^C
3172 GACGTC
AccI 1438 GTATAC GT^[AC][GT]AC
3838 GTCTAC
BamHI 1941 GGATCC G^GATCC
3045 GGATCC
BsaI 28 GAGACC GGTCTC(1/5)
2308 GAGACC
BsaWI 2348 TCCGGA [AT]^CCGG[AT]
3344 TCCGGT
BstAPI 1399 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3525 GCACCGGCTGC
HaeII 952 GGCGCC [GA]GCGC^[CT]
1481 AGCGCT
MseI 2999 TTAA T^TAA
3365 TTAA
SacI 3289 GAGCTC GAGCT^C
3828 GAGCTC
SapI 3460 GAAGAGC GCTCTTC(1/4)
1241 GCTCTTC
StuI 3158 AGGCCT AGG^CCT
3977 AGGCCT
TaqI 3509 TCGA T^CGA
3845 TCGA
Tru9I 2999 TTAA T^TAA
3365 TTAA
Tsp509I 1183 AATT ^AATT
3281 AATT
TthHB8I 3509 TCGA T^CGA
3845 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StyI TseI Tsp45I
TspRI Van91I XcmI XhoII XmaCI
XmaI