Restriction map for
pF1KSDA0353
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccIII AclI AflII AocI
Aor51HI AscI AseI AsnI BamHI
BbeI BbvCI BclI BfrI BglII
BseAI BsiWI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eco47III
Eco81I EcoRV FbaI FseI HindIII
HpaI KasI MluI MroI NarI
NheI NruI NspV PacI PmeI
PshBI Psp1406I RcaI RsrII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 3769 GGTACC G^GTACC
AflIII 74 ACGTGT A^C[GA][CT]GT
AgeI 1819 ACCGGT A^CCGGT
AhdI 4609 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3769 GGTACC G^GTACC
AspEI 4609 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4214 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 342 TGCGCA TGC^GCA
AvrII 3567 CCTAGG C^CTAGG
BalI 854 TGGCCA TGG^CCA
BbrPI 2440 CACGTG CAC^GTG
BlnI 3567 CCTAGG C^CTAGG
BsaI 4535 GAGACC GGTCTC(1/5)
BsmI 3185 GCATTC GAATGC(1/-1)
BssSI 1512 CACGAG CACGAG(-5/-1)
BstAPI 1438 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3466 GGTGACC G^GT[ACGT]ACC
BstPI 3466 GGTGACC G^GT[ACGT]ACC
DrdI 1993 GACACAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4609 GACCAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3466 GGTGACC G^GT[ACGT]ACC
EcoT22I 490 ATGCAT ATGCA^T
FspI 342 TGCGCA TGC^GCA
KpnI 3769 GGTACC GGTAC^C
KspI 315 CCGCGG CCGC^GG
MfeI 1397 CAATTG C^AATTG
MluNI 854 TGGCCA TGG^CCA
MscI 854 TGGCCA TGG^CCA
MunI 1397 CAATTG C^AATTG
NaeI 134 GCCGGC GCC^GGC
NdeI 3422 CATATG CA^TATG
NgoMIV 134 GCCGGC G^CCGGC
NotI 317 GCGGCCGC GC^GGCCGC
NsiI 490 ATGCAT ATGCA^T
PaeR7I 388 CTCGAG C^TCGAG
PflFI 4214 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1819 ACCGGT A^CCGGT
PmaCI 2440 CACGTG CAC^GTG
PmlI 2440 CACGTG CAC^GTG
PvuI 3033 CGATCG CGAT^CG
SacII 315 CCGCGG CCGC^GG
SapI 1760 GAAGAGC GCTCTTC(1/4)
SbfI 891 CCTGCAGG CCTGCA^GG
Sse8387I 891 CCTGCAGG CCTGCA^GG
Tth111I 4214 GACTAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 388 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 4082 GTAGAC GT^[AC][GT]AC
4606 GTAGAC
AcyI 328 GACGCC G[GA]^CG[CT]C
1791 GACGCC
BbsI 900 GAAGAC GAAGAC(2/6)
3627 GTCTTC
BpuAI 900 GAAGAC GAAGAC(2/6)
3627 GTCTTC
BsaHI 328 GACGCC G[GA]^CG[CT]C
1791 GACGCC
BsaWI 1819 ACCGGT [AT]^CCGG[AT]
3493 ACCGGA
Bsp1407I 3218 TGTACA T^GTACA
3885 TGTACA
BsrDI 4248 CATTGC GCAATG(2/0)
4350 CATTGC
BsrGI 3218 TGTACA T^GTACA
3885 TGTACA
EagI 132 CGGCCG C^GGCCG
318 CGGCCG
EclXI 132 CGGCCG C^GGCCG
318 CGGCCG
Eco52I 132 CGGCCG C^GGCCG
318 CGGCCG
EcoRI 1006 GAATTC G^AATTC
2794 GAATTC
Hin1I 328 GACGCC G[GA]^CG[CT]C
1791 GACGCC
HincII 2230 GTCAAC GT[CT]^[GA]AC
3007 GTCAAC
HindII 2230 GTCAAC GT[CT]^[GA]AC
3007 GTCAAC
MslI 2004 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2802 CACCTCCATG
NcoI 857 CCATGG C^CATGG
3978 CCATGG
NspI 995 ACATGC [GA]CATG^[CT]
3516 GCATGT
SmaI 2515 CCCGGG CCC^GGG
3209 CCCGGG
SmlI 388 CTCGAG C^T[CT][GA]AG
1319 CTCAAG
SspBI 3218 TGTACA T^GTACA
3885 TGTACA
StuI 426 AGGCCT AGG^CCT
4517 AGGCCT
XbaI 1740 TCTAGA T^CTAGA
4656 TCTAGA
XcmI 2967 CCAGGGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3434 CCACTGCAGAAGTGG
XmaCI 2515 CCCGGG C^CCGGG
3209 CCCGGG
XmaI 2515 CCCGGG C^CCGGG
3209 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcgI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI