Restriction map for
pF1KSDA0319
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# NO CUT ENZYMES ( 89 enzymes) #
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Acc65I AccII AccIII AclI AflII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BbrPI BbvCI BcgI BclI BfrI
BglI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BsmI BspEI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
DraI EcoNI EcoO65I EcoRI EcoT22I
FbaI FspI HindIII HpaI KpnI
KspI MamI MluI MroI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI SwaI
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1347 GACGTC GACGT^C
AcsI 1116 GAATTT [GA]^AATT[CT]
AhdI 1202 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 400 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2783 AGCGCT AGC^GCT
ApoI 1116 GAATTT [GA]^AATT[CT]
AspEI 1202 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2434 CCTAGG C^CTAGG
BamHI 2261 GGATCC G^GATCC
BbeI 3 GGCGCC GGCGC^C
BciVI 3082 GTATCC GTATCC(6/5)
BlnI 2434 CCTAGG C^CTAGG
BlpI 843 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 843 GCTCAGC GC^T[ACGT]AGC
BsaI 2047 GGTCTC GGTCTC(1/5)
BsiEI 173 CGGCCG CG[GA][CT]^CG
BsmBI 622 GAGACG CGTCTC(1/5)
BspDI 2299 ATCGAT AT^CGAT
BsrFI 1885 GCCGGC [GA]^CCGG[CT]
Bsu36I 400 CCTGAGG CC^T[ACGT]AGG
CelII 843 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1885 GCCGGC [GA]^CCGG[CT]
ClaI 2299 ATCGAT AT^CGAT
DraIII 2314 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 173 CGGCCG C^GGCCG
Eam1105I 1202 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 173 CGGCCG C^GGCCG
Eco47III 2783 AGCGCT AGC^GCT
Eco52I 173 CGGCCG C^GGCCG
Eco81I 400 CCTGAGG CC^T[ACGT]AGG
EcoRV 406 GATATC GAT^ATC
FauI 138 GCGGG CCCGC(4/6)
FseI 1884 GGCCGGCC GGCCGG^CC
KasI 3 GGCGCC G^GCGCC
NaeI 1885 GCCGGC GCC^GGC
NarI 3 GGCGCC GG^CGCC
NcoI 2757 CCATGG C^CATGG
NgoMIV 1885 GCCGGC G^CCGGC
SbfI 2463 CCTGCAGG CCTGCA^GG
SexAI 233 ACCTGGT A^CC[AT]GGT
SfoI 3 GGCGCC GGC^GCC
SmlI 2715 CTTGAG C^T[CT][GA]AG
Sse8387I 2463 CCTGCAGG CCTGCA^GG
SspI 2017 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 1719 ACATGT A^C[GA][CT]GT
2178 ACATGT
BalI 2271 TGGCCA TGG^CCA
2754 TGGCCA
BanI 3 GGCGCC G^G[CT][GA]CC
612 GGTGCC
BseRI 588 CTCCTC GAGGAG(10/8)
451 GAGGAG
Bsp1407I 2354 TGTACA T^GTACA
2610 TGTACA
BspLU11I 1719 ACATGT A^CATGT
2178 ACATGT
BsrDI 29 CATTGC GCAATG(2/0)
1765 GCAATG
BsrGI 2354 TGTACA T^GTACA
2610 TGTACA
BstAPI 52 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2930 GCATCTGCTGC
DrdI 1453 GACTCTCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2551 GACATTAAGGTC
HgaI 624 GACGC GACGC(5/10)
1743 GACGC
MfeI 47 CAATTG C^AATTG
1189 CAATTG
MluNI 2271 TGGCCA TGG^CCA
2754 TGGCCA
MscI 2271 TGGCCA TGG^CCA
2754 TGGCCA
MunI 47 CAATTG C^AATTG
1189 CAATTG
PflMI 2281 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2313 CCACAGTGTGG
SmaI 888 CCCGGG CCC^GGG
2162 CCCGGG
SspBI 2354 TGTACA T^GTACA
2610 TGTACA
StuI 325 AGGCCT AGG^CCT
1064 AGGCCT
TaqI 218 TCGA T^CGA
2300 TCGA
TthHB8I 218 TCGA T^CGA
2300 TCGA
Van91I 2281 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2313 CCACAGTGTGG
XcmI 194 CCAGCTGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2114 CCACCCTCACTGTGG
XmaCI 888 CCCGGG C^CCGGG
2162 CCCGGG
XmaI 888 CCCGGG C^CCGGG
2162 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
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AccI AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII