Restriction map for
pF1KSDA0316
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AhdI Alw44I ApaLI AscI
AspEI AviII AvrII BamHI BbeI
BbvCI BclI BfrI BglI BglII
BlnI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmI BspEI BspLU11I
BssHII BstAPI BstBI BstEII BstPI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI MamI MluI MroI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI Psp1406I PvuI RsrII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1179 GGTACC G^GTACC
AccI 1658 GTATAC GT^[AC][GT]AC
AgeI 378 ACCGGT A^CCGGT
AocI 3541 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1298 AGCGCT AGC^GCT
ApaI 1324 GGGCCC GGGCC^C
AseI 367 ATTAAT AT^TAAT
AsnI 367 ATTAAT AT^TAAT
Asp700I 2166 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1179 GGTACC G^GTACC
BbrPI 1453 CACGTG CAC^GTG
BcgI 28 GCAAAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1528 GGATAC GTATCC(6/5)
BsaAI 1453 CACGTG [CT]AC^GT[GA]
BsaWI 378 ACCGGT [AT]^CCGG[AT]
BsmBI 2607 CGTCTC CGTCTC(1/5)
BspHI 789 TCATGA T^CATGA
BspMI 3517 ACCTGC ACCTGC(4/8)
Bst1107I 1658 GTATAC GTA^TAC
BstZ17I 1658 GTATAC GTA^TAC
Bsu36I 3541 CCTGAGG CC^T[ACGT]AGG
Eco47III 1298 AGCGCT AGC^GCT
Eco81I 3541 CCTGAGG CC^T[ACGT]AGG
EcoNI 2362 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KpnI 1179 GGTACC GGTAC^C
KspI 2180 CCGCGG CCGC^GG
MfeI 881 CAATTG C^AATTG
MunI 881 CAATTG C^AATTG
NaeI 2301 GCCGGC GCC^GGC
NgoMIV 2301 GCCGGC G^CCGGC
NspI 2801 GCATGT [GA]CATG^[CT]
PinAI 378 ACCGGT A^CCGGT
PmaCI 1453 CACGTG CAC^GTG
PmlI 1453 CACGTG CAC^GTG
PshBI 367 ATTAAT AT^TAAT
PvuII 1825 CAGCTG CAG^CTG
RcaI 789 TCATGA T^CATGA
SacII 2180 CCGCGG CCGC^GG
SexAI 315 ACCAGGT A^CC[AT]GGT
XbaI 2625 TCTAGA T^CTAGA
XcmI 1358 CCAAAACCAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2166 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 2181 CGCG CG^CG
2241 CGCG
AcsI 2136 AAATTC [GA]^AATT[CT]
3190 AAATTT
ApoI 2136 AAATTC [GA]^AATT[CT]
3190 AAATTT
BlpI 1827 GCTGAGC GC^T[ACGT]AGC
1876 GCTCAGC
Bpu1102I 1827 GCTGAGC GC^T[ACGT]AGC
1876 GCTCAGC
Bsp1407I 1013 TGTACA T^GTACA
1784 TGTACA
BspDI 409 ATCGAT AT^CGAT
435 ATCGAT
BsrBI 3712 CCGCTC CCGCTC(-3/-3)
400 GAGCGG
BsrGI 1013 TGTACA T^GTACA
1784 TGTACA
BssSI 3209 CACGAG CACGAG(-5/-1)
2278 CTCGTG
BstUI 2181 CGCG CG^CG
2241 CGCG
CelII 1827 GCTGAGC GC^T[ACGT]AGC
1876 GCTCAGC
CfoI 1299 GCGC GCG^C
2567 GCGC
ClaI 409 ATCGAT AT^CGAT
435 ATCGAT
DraIII 314 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1755 CACCATGTG
HaeII 1298 AGCGCT [GA]GCGC^[CT]
2566 AGCGCC
HhaI 1299 GCGC GCG^C
2567 GCGC
HinP1I 1299 GCGC G^CGC
2567 GCGC
HincII 1399 GTCAAC GT[CT]^[GA]AC
3218 GTCAAC
HindII 1399 GTCAAC GT[CT]^[GA]AC
3218 GTCAAC
HindIII 31 AAGCTT A^AGCTT
3535 AAGCTT
MslI 2982 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3696 CACACCCGTG
MvnI 2181 CGCG CG^CG
2241 CGCG
PflMI 1999 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2124 CCAGTTTGTGG
PstI 663 CTGCAG CTGCA^G
3628 CTGCAG
SmaI 345 CCCGGG CCC^GGG
1837 CCCGGG
SmlI 90 CTTGAG C^T[CT][GA]AG
1228 CTCAAG
SspBI 1013 TGTACA T^GTACA
1784 TGTACA
Van91I 1999 CCAACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2124 CCAGTTTGTGG
XmaCI 345 CCCGGG C^CCGGG
1837 CCCGGG
XmaI 345 CCCGGG C^CCGGG
1837 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflFI PleI
PpuMI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XhoII