Restriction map for
pF1KSDA0315
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# NO CUT ENZYMES ( 61 enzymes) #
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Acc65I AccIII AflII AgeI AscI
AseI AsnI Asp718I AvrII BbvCI
BciVI BfaI BfrI BlnI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EcoT22I HpaI KpnI MaeI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RcaI RsrII SalI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI Tru9I XbaI
XhoI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AclI 2549 AACGTT AA^CGTT
AcsI 3754 GAATTC [GA]^AATT[CT]
AflIII 3134 ACGTGT A^C[GA][CT]GT
AhdI 4588 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3404 AGCGCT AGC^GCT
ApoI 3754 GAATTC [GA]^AATT[CT]
AspEI 4588 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4169 TGCGCA TGC^GCA
BbeI 4932 GGCGCC GGCGC^C
BclI 3890 TGATCA T^GATCA
BsaBI 4212 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 153 GAATGC GAATGC(1/-1)
Bsp1407I 4262 TGTACA T^GTACA
BsrGI 4262 TGTACA T^GTACA
BssHII 366 GCGCGC G^CGCGC
Eam1105I 4588 GACCTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3404 AGCGCT AGC^GCT
EcoRI 3754 GAATTC G^AATTC
EcoRV 1042 GATATC GAT^ATC
FbaI 3890 TGATCA T^GATCA
FseI 2520 GGCCGGCC GGCCGG^CC
FspI 4169 TGCGCA TGC^GCA
HindIII 2976 AAGCTT A^AGCTT
KasI 4932 GGCGCC G^GCGCC
MamI 4212 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 4932 GGCGCC GG^CGCC
NruI 3537 TCGCGA TCG^CGA
PshAI 1990 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2549 AACGTT AA^CGTT
SfiI 1509 GGCCGAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4932 GGCGCC GGC^GCC
SspBI 4262 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 20 enzymes) #
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AatII 2626 GACGTC GACGT^C
4593 GACGTC
AccI 3649 GTCTAC GT^[AC][GT]AC
4042 GTCTAC
Asp700I 420 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3750 GAAGGAATTC
AspI 280 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACCGCGTC
BbrPI 1765 CACGTG CAC^GTG
2101 CACGTG
BcgI 3365 CGATGACGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2237 GCAAGAATATCG
BglII 4856 AGATCT A^GATCT
5231 AGATCT
BlpI 3591 GCTCAGC GC^T[ACGT]AGC
5187 GCTGAGC
Bpu1102I 3591 GCTCAGC GC^T[ACGT]AGC
5187 GCTGAGC
BsaWI 566 ACCGGA [AT]^CCGG[AT]
5057 TCCGGT
BsrDI 437 GCAATG GCAATG(2/0)
518 GCAATG
CelII 3591 GCTCAGC GC^T[ACGT]AGC
5187 GCTGAGC
NcoI 3044 CCATGG C^CATGG
3623 CCATGG
NspI 743 GCATGT [GA]CATG^[CT]
5106 GCATGT
PflFI 280 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACCGCGTC
PmaCI 1765 CACGTG CAC^GTG
2101 CACGTG
PmlI 1765 CACGTG CAC^GTG
2101 CACGTG
SacI 471 GAGCTC GAGCT^C
4123 GAGCTC
Tth111I 280 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3844 GACCGCGTC
XmnI 420 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3750 GAAGGAATTC
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# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I KspI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacII SapI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI