Restriction map for
pF1KSDA0286
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BlnI BsaAI BsaHI
BsgI BsiEI BsiWI BsmI BspDI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MfeI MluI
MluNI MscI MunI MvnI MwoI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 115 GTCTAC GT^[AC][GT]AC
AccIII 467 TCCGGA T^CCGGA
AlwNI 958 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1081 GAGCTC G[AT]GC[AT]^C
BbsI 1122 GAAGAC GAAGAC(2/6)
BglII 722 AGATCT A^GATCT
BlpI 537 GCTGAGC GC^T[ACGT]AGC
BpmI 726 CTGGAG CTGGAG(16/14)
Bpu1102I 537 GCTGAGC GC^T[ACGT]AGC
BpuAI 1122 GAAGAC GAAGAC(2/6)
BsaBI 246 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 24 GGTCTC GGTCTC(1/5)
BsaWI 467 TCCGGA [AT]^CCGG[AT]
BseAI 467 TCCGGA T^CCGGA
BsiHKAI 1081 GAGCTC G[AT]GC[AT]^C
BsmBI 1018 CGTCTC CGTCTC(1/5)
Bsp1407I 965 TGTACA T^GTACA
BspEI 467 TCCGGA T^CCGGA
BspHI 776 TCATGA T^CATGA
BsrDI 921 CATTGC GCAATG(2/0)
BsrFI 30 GCCGGC [GA]^CCGG[CT]
BsrGI 965 TGTACA T^GTACA
CelII 537 GCTGAGC GC^T[ACGT]AGC
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
EarI 1176 CTCTTC CTCTTC(1/4)
EcoT14I 809 CCTTGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
HincII 385 GTTAAC GT[CT]^[GA]AC
HindII 385 GTTAAC GT[CT]^[GA]AC
HpaI 385 GTTAAC GTT^AAC
Ksp632I 1176 CTCTTC CTCTTC(1/4)
MamI 246 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 467 TCCGGA T^CCGGA
NaeI 30 GCCGGC GCC^GGC
NgoMIV 30 GCCGGC G^CCGGC
PstI 975 CTGCAG CTGCA^G
RcaI 776 TCATGA T^CATGA
SacI 1081 GAGCTC GAGCT^C
SapI 1175 GCTCTTC GCTCTTC(1/4)
SfcI 975 CTGCAG C^T[GA][CT]AG
SmaI 43 CCCGGG CCC^GGG
SpeI 699 ACTAGT A^CTAGT
SspBI 965 TGTACA T^GTACA
StyI 809 CCTTGG C^C[AT][AT]GG
Tsp45I 283 GTCAC ^GT[GC]AC
XmaCI 43 CCCGGG C^CCGGG
XmaI 43 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 46 GGGCCC GGGCC^C
805 GGGCCC
AvaI 43 CCCGGG C^[CT]CG[GA]G
57 CTCGGG
AvaII 40 GGTCC G^G[AT]CC
845 GGACC
BbvI 852 GCAGC GCAGC(8/12)
107 GCTGC
BciVI 245 GGATAC GTATCC(6/5)
1043 GGATAC
BcnI 43 CCCGG CC^[GC]GG
44 CCGGG
BfaI 319 CTAG C^TAG
700 CTAG
BsoBI 43 CCCGGG C^[CT]CG[GA]G
57 CTCGGG
BstYI 251 AGATCC [GA]^GATC[CT]
722 AGATCT
DraI 357 TTTAAA TTT^AAA
706 TTTAAA
DraII 46 GGGCCCT [GA]G^G[ACGT]CC[CT]
805 GGGCCCT
EcoO109I 46 GGGCCCT [GA]G^G[ACGT]CC[CT]
805 GGGCCCT
HphI 284 TCACC GGTGA(8/7)
971 TCACC
MaeI 319 CTAG C^TAG
700 CTAG
MaeII 389 ACGT A^CGT
653 ACGT
MflI 251 AGATCC [GA]^GATC[CT]
722 AGATCT
MspA1I 125 CAGCTG C[AC]G^C[GT]G
853 CAGCTG
NciI 43 CCCGG CC^[GC]GG
44 CCGGG
PvuII 125 CAGCTG CAG^CTG
853 CAGCTG
SfaNI 884 GCATC GCATC(5/9)
1267 GCATC
TaqI 279 TCGA T^CGA
1134 TCGA
TseI 107 GCTGC G^C[AT]GC
852 GCAGC
TthHB8I 279 TCGA T^CGA
1134 TCGA
XhoII 251 AGATCC [GA]^GATC[CT]
722 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanII BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI TfiI Tru9I
Tsp509I TspRI