Restriction map for
pF1KSDA0278
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I EarI Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SalI SapI SbfI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tsp509I Tth111I XbaI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AflIII 341 ACGTGT A^C[GA][CT]GT
AgeI 194 ACCGGT A^CCGGT
Asp700I 804 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 118 GAGCAC G[AT]GC[AT]^C
BfaI 670 CTAG C^TAG
BglI 1087 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 635 AGATCT A^GATCT
BsiHKAI 118 GAGCAC G[AT]GC[AT]^C
DrdI 496 GACTACACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1074 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HphI 521 TCACC GGTGA(8/7)
KspI 49 CCGCGG CCGC^GG
MaeI 670 CTAG C^TAG
MaeIII 1169 GTGAC ^GT[ACGT]AC
MslI 1056 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 650 CTCGAG C^TCGAG
PinAI 194 ACCGGT A^CCGGT
SacII 49 CCGCGG CCGC^GG
SfiI 1086 GGCCGAGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 275 AGGCCT AGG^CCT
TfiI 729 GAATC G^A[AT]TC
Tsp45I 1169 GTGAC ^GT[GC]AC
TspRI 1167 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 650 CTCGAG C^TCGAG
XmnI 804 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 81 GATCC GGATC(4/5)
723 GATCC
AlwNI 533 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
898 CAGTTCCTG
BalI 65 TGGCCA TGG^CCA
1163 TGGCCA
BanI 140 GGCACC G^G[CT][GA]CC
979 GGCACC
BbrPI 121 CACGTG CAC^GTG
340 CACGTG
BsaI 298 GAGACC GGTCTC(1/5)
1129 GAGACC
BsgI 190 CTGCAC GTGCAG(16/14)
1063 GTGCAG
BsmAI 298 GAGAC GTCTC(1/5)
1129 GAGAC
BssSI 240 CACGAG CACGAG(-5/-1)
478 CACGAG
BstXI 161 CCAAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCAAGACCCTGG
BstYI 635 AGATCT [GA]^GATC[CT]
722 AGATCC
DdeI 661 CTGAG C^T[ACGT]AG
701 CTGAG
EagI 1085 CGGCCG C^GGCCG
1094 CGGCCG
EclXI 1085 CGGCCG C^GGCCG
1094 CGGCCG
Eco52I 1085 CGGCCG C^GGCCG
1094 CGGCCG
FseI 743 GGCCGGCC GGCCGG^CC
1095 GGCCGGCC
MflI 635 AGATCT [GA]^GATC[CT]
722 AGATCC
MluNI 65 TGGCCA TGG^CCA
1163 TGGCCA
MscI 65 TGGCCA TGG^CCA
1163 TGGCCA
NlaIII 735 CATG CATG^
1056 CATG
PmaCI 121 CACGTG CAC^GTG
340 CACGTG
PmlI 121 CACGTG CAC^GTG
340 CACGTG
ScaI 677 AGTACT AGT^ACT
707 AGTACT
SfaNI 111 GATGC GCATC(5/9)
336 GATGC
SmaI 43 CCCGGG CCC^GGG
550 CCCGGG
SmlI 650 CTCGAG C^T[CT][GA]AG
997 CTCAAG
TaqI 641 TCGA T^CGA
651 TCGA
TthHB8I 641 TCGA T^CGA
651 TCGA
XcmI 302 CCATCGCCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCAGCCCCGGCGTGG
XhoII 635 AGATCT [GA]^GATC[CT]
722 AGATCC
XmaCI 43 CCCGGG C^CCGGG
550 CCCGGG
XmaI 43 CCCGGG C^CCGGG
550 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AfaI AluI Aor51HI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaAI BsaJI BsaWI
BseRI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DsaI EaeI Eco47III EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII ItaI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfcI
TseI Van91I