Restriction map for
pF1KSDA0273
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII Bst1107I
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SapI SbfI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 166 GACGTC GACGT^C
AccII 751 CGCG CG^CG
AcsI 141 GAATTC [GA]^AATT[CT]
AlwI 621 GATCC GGATC(4/5)
ApoI 141 GAATTC [GA]^AATT[CT]
AspHI 352 GAGCAC G[AT]GC[AT]^C
BalI 185 TGGCCA TGG^CCA
BbeI 717 GGCGCC GGCGC^C
BcnI 162 CCGGG CC^[GC]GG
BsiHKAI 352 GAGCAC G[AT]GC[AT]^C
BsmAI 316 GAGAC GTCTC(1/5)
BsmBI 316 GAGACG CGTCTC(1/5)
Bsp1407I 683 TGTACA T^GTACA
BspHI 920 TCATGA T^CATGA
BsrDI 774 CATTGC GCAATG(2/0)
BsrGI 683 TGTACA T^GTACA
BssSI 181 CTCGTG CACGAG(-5/-1)
BstAPI 962 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 99 GGTCACC G^GT[ACGT]ACC
BstPI 99 GGTCACC G^GT[ACGT]ACC
BstUI 751 CGCG CG^CG
BstXI 176 CCAAGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 620 AGATCC [GA]^GATC[CT]
EagI 603 CGGCCG C^GGCCG
EarI 637 CTCTTC CTCTTC(1/4)
EclXI 603 CGGCCG C^GGCCG
Eco52I 603 CGGCCG C^GGCCG
EcoO65I 99 GGTCACC G^GT[ACGT]ACC
EcoRI 141 GAATTC G^AATTC
HincII 874 GTCGAC GT[CT]^[GA]AC
HindII 874 GTCGAC GT[CT]^[GA]AC
KasI 717 GGCGCC G^GCGCC
Ksp632I 637 CTCTTC CTCTTC(1/4)
MaeII 167 ACGT A^CGT
MflI 620 AGATCC [GA]^GATC[CT]
MluNI 185 TGGCCA TGG^CCA
MscI 185 TGGCCA TGG^CCA
MseI 122 TTAA T^TAA
MvnI 751 CGCG CG^CG
NarI 717 GGCGCC GG^CGCC
NciI 162 CCGGG CC^[GC]GG
NcoI 74 CCATGG C^CATGG
RcaI 920 TCATGA T^CATGA
SalI 874 GTCGAC G^TCGAC
ScaI 440 AGTACT AGT^ACT
SexAI 296 ACCTGGT A^CC[AT]GGT
SfoI 717 GGCGCC GGC^GCC
SspBI 683 TGTACA T^GTACA
TaqI 875 TCGA T^CGA
Tru9I 122 TTAA T^TAA
Tsp509I 142 AATT ^AATT
TthHB8I 875 TCGA T^CGA
XhoII 620 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 874 GTCGAC GT^[AC][GT]AC
930 GTCTAC
AcyI 166 GACGTC G[GA]^CG[CT]C
717 GGCGCC
BanII 234 GAGCCC G[GA]GC[CT]^C
868 GAGCCC
BsaHI 166 GACGTC G[GA]^CG[CT]C
717 GGCGCC
BsgI 330 CTGCAC GTGCAG(16/14)
262 GTGCAG
BsiEI 603 CGGCCG CG[GA][CT]^CG
753 CGACCG
BsrI 224 ACTGG ACTGG(1/-1)
334 ACTGG
HapII 162 CCGG C^CGG
305 CCGG
Hin1I 166 GACGTC G[GA]^CG[CT]C
717 GGCGCC
HpaII 162 CCGG C^CGG
305 CCGG
MaeIII 86 GTGAC ^GT[ACGT]AC
100 GTCAC
MspI 162 CCGG C^CGG
305 CCGG
PstI 478 CTGCAG CTGCA^G
798 CTGCAG
PvuII 552 CAGCTG CAG^CTG
957 CAGCTG
SfaNI 940 GATGC GCATC(5/9)
62 GCATC
SfcI 478 CTGCAG C^T[GA][CT]AG
798 CTGCAG
SphI 398 GCATGC GCATG^C
450 GCATGC
Tsp45I 86 GTGAC ^GT[GC]AC
100 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbsI BbvI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsiYI BslI
BsmFI Bsp1286I BspMI BssKI BstF5I
BstNI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HinfI HphI ItaI MboI MboII
MnlI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
StyI TseI TspRI XcmI