Restriction map for
pF1KSDA0271
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HindIII HinfI HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MboII MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 479 GGCGTC G[GA]^CG[CT]C
AlwNI 110 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 253 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 97 CCCGGG C^[CT]CG[GA]G
BamHI 410 GGATCC G^GATCC
BbvCI 63 GCTGAGG CCTCAGC(-5/-2)
BfaI 572 CTAG C^TAG
BglI 133 GCCATGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 479 GGCGTC G[GA]^CG[CT]C
BseRI 469 GAGGAG GAGGAG(10/8)
BsmBI 391 GAGACG CGTCTC(1/5)
BsoBI 97 CCCGGG C^[CT]CG[GA]G
BstAPI 313 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 374 GGATG GGATG(2/0)
BstXI 414 CCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 410 GGATCC [GA]^GATC[CT]
DraIII 314 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 530 CCGTGG C^C[GA][CT]GG
EcoNI 465 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 374 GGATG GGATG(9/13)
Hin1I 479 GGCGTC G[GA]^CG[CT]C
HincII 331 GTCAAC GT[CT]^[GA]AC
HindII 331 GTCAAC GT[CT]^[GA]AC
HphI 236 TCACC GGTGA(8/7)
MaeI 572 CTAG C^TAG
MflI 410 GGATCC [GA]^GATC[CT]
MslI 415 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 571 GCTAGC G^CTAGC
NspI 204 GCATGT [GA]CATG^[CT]
SfaNI 502 GCATC GCATC(5/9)
SfcI 554 CTGTAG C^T[GA][CT]AG
SmaI 97 CCCGGG CCC^GGG
TaqI 158 TCGA T^CGA
Tsp45I 208 GTGAC ^GT[GC]AC
TthHB8I 158 TCGA T^CGA
XhoII 410 GGATCC [GA]^GATC[CT]
XmaCI 97 CCCGGG C^CCGGG
XmaI 97 CCCGGG C^CCGGG
XmnI 253 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 395 CGCG CG^CG
476 CGCG
AlwI 411 GATCC GGATC(4/5)
410 GGATC
BcnI 97 CCCGG CC^[GC]GG
98 CCGGG
BlpI 196 GCTCAGC GC^T[ACGT]AGC
218 GCTCAGC
Bpu1102I 196 GCTCAGC GC^T[ACGT]AGC
218 GCTCAGC
BsaI 160 GAGACC GGTCTC(1/5)
243 GGTCTC
BsmFI 357 GGGAC GGGAC(10/14)
455 GGGAC
BstUI 395 CGCG CG^CG
476 CGCG
CelII 196 GCTCAGC GC^T[ACGT]AGC
218 GCTCAGC
CfoI 174 GCGC GCG^C
475 GCGC
DpnII 187 GATC ^GATC
411 GATC
HapII 98 CCGG C^CGG
171 CCGG
HgaI 393 GACGC GACGC(5/10)
480 GCGTC
HhaI 174 GCGC GCG^C
475 GCGC
HinP1I 174 GCGC G^CGC
475 GCGC
HpaII 98 CCGG C^CGG
171 CCGG
MaeIII 208 GTGAC ^GT[ACGT]AC
550 GTAAC
MboI 187 GATC ^GATC
411 GATC
MspI 98 CCGG C^CGG
171 CCGG
MvnI 395 CGCG CG^CG
476 CGCG
NciI 97 CCCGG CC^[GC]GG
98 CCGGG
NdeII 187 GATC ^GATC
411 GATC
NlaIII 135 CATG CATG^
205 CATG
Sau3AI 187 GATC ^GATC
411 GATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI ApaI BanII BbvI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII FauI Fnu4HI HaeIII
ItaI MnlI MspA1I MvaI MwoI
NlaIV PvuII Sau96I ScrFI TseI
TspRI