Restriction map for
pF1KSDA0264
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HpaI
HpaII HphI KasI KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI Sau96I
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StyI SwaI Tsp45I
Tth111I XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 305 GGTACC G^GTACC
AccI 103 GTAGAC GT^[AC][GT]AC
AcsI 407 GAATTT [GA]^AATT[CT]
AlwI 1167 GATCC GGATC(4/5)
AlwNI 1129 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 834 AGCGCT AGC^GCT
ApoI 407 GAATTT [GA]^AATT[CT]
Asp718I 305 GGTACC G^GTACC
AvaI 242 CTCGGG C^[CT]CG[GA]G
BanI 305 GGTACC G^G[CT][GA]CC
BanII 773 GAGCCC G[GA]GC[CT]^C
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BfaI 1150 CTAG C^TAG
BmyI 773 GAGCCC G[AGT]GC[ACT]^C
BsaJI 921 CCAGGG C^C[ACGT][ACGT]GG
BsmFI 900 GTCCC GGGAC(10/14)
BsoBI 242 CTCGGG C^[CT]CG[GA]G
Bsp1286I 773 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 1158 TGTACA T^GTACA
BspHI 500 TCATGA T^CATGA
BsrDI 562 CATTGC GCAATG(2/0)
BsrGI 1158 TGTACA T^GTACA
BstAPI 1128 GCAGAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 669 CCAGTTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1194 GCAAGC GC[ACGT]^[ACGT]GC
Eco47III 834 AGCGCT AGC^GCT
EcoNI 332 CCTGGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 23 GCGGG CCCGC(4/6)
HaeII 834 AGCGCT [GA]GCGC^[CT]
KpnI 305 GGTACC GGTAC^C
MaeI 1150 CTAG C^TAG
MspA1I 875 CAGCTG C[AC]G^C[GT]G
NlaIV 305 GGTACC GG[ACGT]^[ACGT]CC
NspI 737 ACATGC [GA]CATG^[CT]
PflMI 296 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1042 GAGTC GAGTC(4/5)
PvuII 875 CAGCTG CAG^CTG
RcaI 500 TCATGA T^CATGA
SbfI 67 CCTGCAGG CCTGCA^GG
Sse8387I 67 CCTGCAGG CCTGCA^GG
SspBI 1158 TGTACA T^GTACA
Van91I 296 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1149 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AccII 22 CGCG CG^CG
38 CGCG
BseRI 598 GAGGAG GAGGAG(10/8)
892 GAGGAG
BspMI 83 ACCTGC ACCTGC(4/8)
70 GCAGGT
BstUI 22 CGCG CG^CG
38 CGCG
EarI 247 GAAGAG CTCTTC(1/4)
595 GAAGAG
HaeIII 634 GGCC GG^CC
1005 GGCC
HindIII 976 AAGCTT A^AGCTT
1018 AAGCTT
Ksp632I 247 GAAGAG CTCTTC(1/4)
595 GAAGAG
MvnI 22 CGCG CG^CG
38 CGCG
PstI 68 CTGCAG CTGCA^G
1135 CTGCAG
SfcI 68 CTGCAG C^T[GA][CT]AG
1135 CTGCAG
StuI 633 AGGCCT AGG^CCT
1004 AGGCCT
TfiI 445 GATTC G^A[AT]TC
1131 GAATC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI BbsI BbvI
BpuAI BsiYI BslI BsrI BssKI
BstF5I BstNI CfoI DdeI DpnII
EcoRII Fnu4HI FokI HhaI HinP1I
HinfI ItaI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I