Restriction map for
pF1KSDA0258
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 846 ACATGT A^C[GA][CT]GT
AlwI 316 GATCC GGATC(4/5)
AlwNI 1052 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 306 CCTGAGG CC^T[ACGT]AGG
AspHI 19 GAGCTC G[AT]GC[AT]^C
AvaI 992 CCCGAG C^[CT]CG[GA]G
BanI 863 GGCACC G^G[CT][GA]CC
BanII 19 GAGCTC G[GA]GC[CT]^C
BciVI 269 GTATCC GTATCC(6/5)
BsaWI 500 TCCGGT [AT]^CCGG[AT]
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
BsoBI 992 CCCGAG C^[CT]CG[GA]G
BspLU11I 846 ACATGT A^CATGT
BspMI 1091 ACCTGC ACCTGC(4/8)
Bsu36I 306 CCTGAGG CC^T[ACGT]AGG
CfoI 55 GCGC GCG^C
DpnII 316 GATC ^GATC
DsaI 95 CCACGG C^C[GA][CT]GG
Eco81I 306 CCTGAGG CC^T[ACGT]AGG
HaeII 54 GGCGCT [GA]GCGC^[CT]
HhaI 55 GCGC GCG^C
HinP1I 55 GCGC G^CGC
HincII 433 GTCAAC GT[CT]^[GA]AC
HindII 433 GTCAAC GT[CT]^[GA]AC
MboI 316 GATC ^GATC
MseI 727 TTAA T^TAA
NdeI 1133 CATATG CA^TATG
NdeII 316 GATC ^GATC
NheI 579 GCTAGC G^CTAGC
NspI 846 ACATGT [GA]CATG^[CT]
PshAI 196 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1083 CAGCTG CAG^CTG
SacI 19 GAGCTC GAGCT^C
Sau3AI 316 GATC ^GATC
TaqI 174 TCGA T^CGA
Tru9I 727 TTAA T^TAA
TthHB8I 174 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 403 GTCTAC GT^[AC][GT]AC
1072 GTAGAC
AcsI 290 AAATTC [GA]^AATT[CT]
979 GAATTT
ApoI 290 AAATTC [GA]^AATT[CT]
979 GAATTT
BcnI 27 CCGGG CC^[GC]GG
860 CCCGG
BlpI 21 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
BmyI 19 GAGCTC G[AGT]GC[ACT]^C
137 GTGCCC
BpmI 58 CTGGAG CTGGAG(16/14)
320 CTGGAG
Bpu1102I 21 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
Bsp1286I 19 GAGCTC G[AGT]GC[ACT]^C
137 GTGCCC
CelII 21 GCTCAGC GC^T[ACGT]AGC
583 GCTGAGC
EaeI 98 CGGCCA [CT]^GGCC[GA]
654 TGGCCG
HphI 253 GGTGA GGTGA(8/7)
77 TCACC
MboII 564 GAAGA GAAGA(8/7)
683 TCTTC
MspA1I 808 CAGCGG C[AC]G^C[GT]G
1083 CAGCTG
NciI 27 CCGGG CC^[GC]GG
860 CCCGG
SfaNI 109 GCATC GCATC(5/9)
680 GCATC
TfiI 571 GATTC G^A[AT]TC
871 GAATC
Tsp509I 291 AATT ^AATT
980 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BsaJI BseRI BsiYI BslI
BsmFI Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeII MaeIII MnlI MslI
MspI MvaI MwoI NlaIII NlaIV
PleI PpuMI RsaI Sau96I ScrFI
SfcI SspBI TseI Tsp45I TspRI
XcmI