Restriction map for
pF1KSDA0253
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I Aor51HI
ApaLI AscI Asp700I Asp718I AspI
AviII AvrII BbeI BbrPI BbvCI
BciVI BglII BlnI BsaHI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I Cfr10I ClaI DraI DrdI
EagI EclXI Eco47III Eco52I FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI TfiI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 1030 GTCTAC GT^[AC][GT]AC
AflII 1107 CTTAAG C^TTAAG
ApaI 1818 GGGCCC GGGCC^C
AseI 1387 ATTAAT AT^TAAT
AsnI 1387 ATTAAT AT^TAAT
BamHI 1761 GGATCC G^GATCC
BcgI 1747 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2009 TGATCA T^GATCA
BfrI 1107 CTTAAG C^TTAAG
BglI 874 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 220 CACGTA [CT]AC^GT[GA]
BsaBI 117 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 46 GGTCTC GGTCTC(1/5)
BseRI 235 GAGGAG GAGGAG(10/8)
BsmBI 1837 GAGACG CGTCTC(1/5)
BsmI 1827 GCATTC GAATGC(1/-1)
BstEII 1551 GGTTACC G^GT[ACGT]ACC
BstPI 1551 GGTTACC G^GT[ACGT]ACC
CpoI 1841 CGGACCG CG^G[AT]CCG
DraIII 632 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1472 CCACGG C^C[GA][CT]GG
EcoO65I 1551 GGTTACC G^GT[ACGT]ACC
EcoRI 492 GAATTC G^AATTC
EcoRV 1963 GATATC GAT^ATC
EcoT22I 661 ATGCAT ATGCA^T
FbaI 2009 TGATCA T^GATCA
HgaI 71 GCGTC GACGC(5/10)
HindIII 929 AAGCTT A^AGCTT
MamI 117 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 661 ATGCAT ATGCA^T
PaeR7I 1020 CTCGAG C^TCGAG
PflMI 421 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1387 ATTAAT AT^TAAT
PvuII 913 CAGCTG CAG^CTG
RsrII 1841 CGGACCG CG^G[AT]CCG
ScaI 1802 AGTACT AGT^ACT
SexAI 1175 ACCAGGT A^CC[AT]GGT
SspI 1352 AATATT AAT^ATT
Van91I 421 CCAAATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 311 CCAGGGATTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1020 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcsI 492 GAATTC [GA]^AATT[CT]
1716 AAATTT
AhdI 745 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1621 GACCTAAGGTC
ApoI 492 GAATTC [GA]^AATT[CT]
1716 AAATTT
AspEI 745 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1621 GACCTAAGGTC
BalI 1193 TGGCCA TGG^CCA
1469 TGGCCA
BanI 942 GGCACC G^G[CT][GA]CC
1336 GGTGCC
BfaI 79 CTAG C^TAG
407 CTAG
BlpI 614 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
BpmI 1671 CTCCAG CTGGAG(16/14)
292 CTGGAG
Bpu1102I 614 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
Bsp1407I 411 TGTACA T^GTACA
1698 TGTACA
BspMI 685 ACCTGC ACCTGC(4/8)
82 GCAGGT
BsrGI 411 TGTACA T^GTACA
1698 TGTACA
CelII 614 GCTCAGC GC^T[ACGT]AGC
1410 GCTGAGC
CfoI 696 GCGC GCG^C
890 GCGC
EaeI 1193 TGGCCA [CT]^GGCC[GA]
1469 TGGCCA
Eam1105I 745 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1621 GACCTAAGGTC
EcoNI 388 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1611 CCTCAGGCAGG
EcoT14I 1451 CCAAGG C^C[AT][AT]GG
1709 CCTTGG
FauI 965 CCCGC CCCGC(4/6)
1556 CCCGC
HhaI 696 GCGC GCG^C
890 GCGC
HinP1I 696 GCGC G^CGC
890 GCGC
HincII 1075 GTTGAC GT[CT]^[GA]AC
1735 GTCAAC
HindII 1075 GTTGAC GT[CT]^[GA]AC
1735 GTCAAC
MaeI 79 CTAG C^TAG
407 CTAG
MaeII 221 ACGT A^CGT
1485 ACGT
MluNI 1193 TGGCCA TGG^CCA
1469 TGGCCA
MscI 1193 TGGCCA TGG^CCA
1469 TGGCCA
MspA1I 913 CAGCTG C[AC]G^C[GT]G
1953 CCGCTG
PstI 474 CTGCAG CTGCA^G
1279 CTGCAG
SacI 1295 GAGCTC GAGCT^C
1920 GAGCTC
SmaI 33 CCCGGG CCC^GGG
2102 CCCGGG
SspBI 411 TGTACA T^GTACA
1698 TGTACA
StyI 1451 CCAAGG C^C[AT][AT]GG
1709 CCTTGG
XmaCI 33 CCCGGG C^CCGGG
2102 CCCGGG
XmaI 33 CCCGGG C^CCGGG
2102 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII EarI Eco81I EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII