Restriction map for
pF1KSDA0217
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# NO CUT ENZYMES ( 90 enzymes) #
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AccI AclI AflII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp700I AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BsaAI BsaBI BspHI
BsrBI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I CpoI DrdI
Eam1105I EarI Eco81I EcoRV EcoT22I
FspI HincII HindII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MspA1I MunI NarI NheI NotI
NruI NsiI NspV PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1014 GACGTC GACGT^C
Acc65I 141 GGTACC G^GTACC
AccIII 1346 TCCGGA T^CCGGA
AflIII 527 ACATGT A^C[GA][CT]GT
AlwNI 842 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 141 GGTACC G^GTACC
AspHI 2190 GAGCAC G[AT]GC[AT]^C
AvaI 467 CCCGAG C^[CT]CG[GA]G
BalI 261 TGGCCA TGG^CCA
BciVI 1215 GTATCC GTATCC(6/5)
BcnI 2079 CCGGG CC^[GC]GG
BglII 643 AGATCT A^GATCT
BlpI 606 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 606 GCTCAGC GC^T[ACGT]AGC
BseAI 1346 TCCGGA T^CCGGA
BsiHKAI 2190 GAGCAC G[AT]GC[AT]^C
BsiWI 1784 CGTACG C^GTACG
BsmI 327 GAATGC GAATGC(1/-1)
BsoBI 467 CCCGAG C^[CT]CG[GA]G
BspDI 1328 ATCGAT AT^CGAT
BspEI 1346 TCCGGA T^CCGGA
BspLU11I 527 ACATGT A^CATGT
BstAPI 214 GCAAGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 310 GGTGACC G^GT[ACGT]ACC
BstPI 310 GGTGACC G^GT[ACGT]ACC
BstYI 643 AGATCT [GA]^GATC[CT]
CelII 606 GCTCAGC GC^T[ACGT]AGC
ClaI 1328 ATCGAT AT^CGAT
DraI 766 TTTAAA TTT^AAA
DraIII 1855 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2131 CGGCCG C^GGCCG
EclXI 2131 CGGCCG C^GGCCG
Eco52I 2131 CGGCCG C^GGCCG
EcoNI 1465 CCTGGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 310 GGTGACC G^GT[ACGT]ACC
FseI 2143 GGCCGGCC GGCCGG^CC
HindIII 1575 AAGCTT A^AGCTT
KpnI 141 GGTACC GGTAC^C
MflI 643 AGATCT [GA]^GATC[CT]
MluNI 261 TGGCCA TGG^CCA
MroI 1346 TCCGGA T^CCGGA
MscI 261 TGGCCA TGG^CCA
NciI 2079 CCGGG CC^[GC]GG
NcoI 2162 CCATGG C^CATGG
NdeI 1406 CATATG CA^TATG
PacI 1333 TTAATTAA TTAAT^TAA
PstI 1322 CTGCAG CTGCA^G
ScaI 474 AGTACT AGT^ACT
SmlI 2095 CTCAAG C^T[CT][GA]AG
SphI 1257 GCATGC GCATG^C
XcmI 267 CCACGCAGACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 643 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 38 enzymes) #
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AccII 1011 CGCG CG^CG
1893 CGCG
AcyI 1014 GACGTC G[GA]^CG[CT]C
1188 GGCGTC
Aor51HI 70 AGCGCT AGC^GCT
1004 AGCGCT
AseI 1332 ATTAAT AT^TAAT
1336 ATTAAT
AsnI 1332 ATTAAT AT^TAAT
1336 ATTAAT
AspI 1086 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1539 GACACAGTC
BanI 141 GGTACC G^G[CT][GA]CC
1755 GGTGCC
BbsI 460 GAAGAC GAAGAC(2/6)
1632 GAAGAC
BclI 9 TGATCA T^GATCA
1073 TGATCA
BpuAI 460 GAAGAC GAAGAC(2/6)
1632 GAAGAC
BsaHI 1014 GACGTC G[GA]^CG[CT]C
1188 GGCGTC
BsaI 2120 GAGACC GGTCTC(1/5)
1737 GGTCTC
BsaWI 1346 TCCGGA [AT]^CCGG[AT]
1502 ACCGGA
BsiEI 1889 CGACCG CG[GA][CT]^CG
2131 CGGCCG
BsmFI 1281 GGGAC GGGAC(10/14)
389 GTCCC
Bsp1407I 1037 TGTACA T^GTACA
1064 TGTACA
BspMI 1369 GCAGGT ACCTGC(4/8)
1908 GCAGGT
BsrDI 335 CATTGC GCAATG(2/0)
1994 CATTGC
BsrGI 1037 TGTACA T^GTACA
1064 TGTACA
BsrI 1144 ACTGG ACTGG(1/-1)
558 CCAGT
BstUI 1011 CGCG CG^CG
1893 CGCG
CfoI 71 GCGC GCG^C
1005 GCGC
Eco47III 70 AGCGCT AGC^GCT
1004 AGCGCT
EcoRI 493 GAATTC G^AATTC
1488 GAATTC
FbaI 9 TGATCA T^GATCA
1073 TGATCA
HaeII 70 AGCGCT [GA]GCGC^[CT]
1004 AGCGCT
HhaI 71 GCGC GCG^C
1005 GCGC
Hin1I 1014 GACGTC G[GA]^CG[CT]C
1188 GGCGTC
HinP1I 71 GCGC G^CGC
1005 GCGC
MvnI 1011 CGCG CG^CG
1893 CGCG
NspI 527 ACATGT [GA]CATG^[CT]
1257 GCATGC
PflFI 1086 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1539 GACACAGTC
PshBI 1332 ATTAAT AT^TAAT
1336 ATTAAT
SfaNI 295 GATGC GCATC(5/9)
850 GATGC
SfcI 1322 CTGCAG C^T[GA][CT]AG
1730 CTGTAG
SspBI 1037 TGTACA T^GTACA
1064 TGTACA
SspI 714 AATATT AAT^ATT
1308 AATATT
Tth111I 1086 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1539 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmBI Bsp1286I
BsrFI BssKI BstF5I BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NaeI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI RsaI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I