Restriction map for
pF1KSDA0207
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp718I AspEI
AspI BamHI BbrPI BciVI BclI
BfrI BglI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
HindIII HpaI KpnI Ksp632I KspI
MroI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 1309 CGCG CG^CG
AflIII 1308 ACGCGT A^C[GA][CT]GT
Alw44I 325 GTGCAC G^TGCAC
AlwNI 982 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 311 AGCGCT AGC^GCT
ApaLI 325 GTGCAC G^TGCAC
Asp700I 759 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1253 TGCGCA TGC^GCA
BbeI 344 GGCGCC GGCGC^C
BsaBI 1386 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 554 GAGACC GGTCTC(1/5)
BspMI 977 ACCTGC ACCTGC(4/8)
BsrDI 1282 GCAATG GCAATG(2/0)
BstAPI 300 GCAGAGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1309 CGCG CG^CG
DraI 745 TTTAAA TTT^AAA
DraII 261 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 311 AGCGCT AGC^GCT
EcoO109I 261 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 161 ATGCAT ATGCA^T
FspI 1253 TGCGCA TGC^GCA
KasI 344 GGCGCC G^GCGCC
MamI 1386 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 565 CAATTG C^AATTG
MluI 1308 ACGCGT A^CGCGT
MunI 565 CAATTG C^AATTG
MvnI 1309 CGCG CG^CG
NarI 344 GGCGCC GG^CGCC
NcoI 689 CCATGG C^CATGG
NsiI 161 ATGCAT ATGCA^T
SfoI 344 GGCGCC GGC^GCC
SpeI 612 ACTAGT A^CTAGT
StuI 1223 AGGCCT AGG^CCT
TaqI 1243 TCGA T^CGA
TthHB8I 1243 TCGA T^CGA
XcmI 792 CCAGCAGTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 759 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 344 GGCGCC G[GA]^CG[CT]C
1245 GACGCC
AvaI 251 CTCGGG C^[CT]CG[GA]G
294 CCCGAG
AvrII 633 CCTAGG C^CTAGG
1395 CCTAGG
BalI 228 TGGCCA TGG^CCA
692 TGGCCA
BbsI 358 GAAGAC GAAGAC(2/6)
655 GAAGAC
BbvCI 1210 CCTCAGC CCTCAGC(-5/-2)
1107 GCTGAGG
BcnI 69 CCCGG CC^[GC]GG
448 CCGGG
BglII 928 AGATCT A^GATCT
1610 AGATCT
BlnI 633 CCTAGG C^CTAGG
1395 CCTAGG
BpuAI 358 GAAGAC GAAGAC(2/6)
655 GAAGAC
BsaHI 344 GGCGCC G[GA]^CG[CT]C
1245 GACGCC
BsgI 307 GTGCAG GTGCAG(16/14)
1121 GTGCAG
BsmAI 554 GAGAC GTCTC(1/5)
1261 GTCTC
BsmI 159 GAATGC GAATGC(1/-1)
1181 GAATGC
BsoBI 251 CTCGGG C^[CT]CG[GA]G
294 CCCGAG
BsrFI 10 GCCGGC [GA]^CCGG[CT]
387 GCCGGC
BssSI 1276 CTCGTG CACGAG(-5/-1)
1516 CTCGTG
Cfr10I 10 GCCGGC [GA]^CCGG[CT]
387 GCCGGC
DraIII 586 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1575 CACACTGTG
DsaI 689 CCATGG C^C[GA][CT]GG
1059 CCACGG
EcoNI 348 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1347 CCTGGAGGAGG
HaeII 311 AGCGCT [GA]GCGC^[CT]
344 GGCGCC
HgaI 1245 GACGC GACGC(5/10)
1307 GACGC
Hin1I 344 GGCGCC G[GA]^CG[CT]C
1245 GACGCC
HincII 59 GTCAAC GT[CT]^[GA]AC
1693 GTTGAC
HindII 59 GTCAAC GT[CT]^[GA]AC
1693 GTTGAC
MaeII 1144 ACGT A^CGT
1639 ACGT
MluNI 228 TGGCCA TGG^CCA
692 TGGCCA
MscI 228 TGGCCA TGG^CCA
692 TGGCCA
MslI 1380 CACACGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1749 CATCCGAGTG
NaeI 10 GCCGGC GCC^GGC
387 GCCGGC
NciI 69 CCCGG CC^[GC]GG
448 CCGGG
NgoMIV 10 GCCGGC G^CCGGC
387 GCCGGC
PflMI 564 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCATGAGCTGG
SfaNI 163 GCATC GCATC(5/9)
1748 GCATC
SmlI 1171 CTCAAG C^T[CT][GA]AG
1735 CTCAAG
SphI 194 GCATGC GCATG^C
234 GCATGC
Tsp45I 584 GTCAC ^GT[GC]AC
1541 GTGAC
Van91I 564 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
660 CCATGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbvI BcgI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII