Restriction map for
pF1KSDA0205
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Aor51HI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BcgI
BclI BcnI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 840 ACATGT A^C[GA][CT]GT
Alw44I 321 GTGCAC G^TGCAC
AlwNI 475 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 561 CCTCAGG CC^T[ACGT]AGG
ApaLI 321 GTGCAC G^TGCAC
Asp700I 413 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 321 GTGCAC G[AT]GC[AT]^C
BamHI 809 GGATCC G^GATCC
BbsI 931 GAAGAC GAAGAC(2/6)
BbvCI 1014 CCTCAGC CCTCAGC(-5/-2)
BciVI 817 GGATAC GTATCC(6/5)
BfaI 424 CTAG C^TAG
BglII 457 AGATCT A^GATCT
BmyI 321 GTGCAC G[AGT]GC[ACT]^C
BpuAI 931 GAAGAC GAAGAC(2/6)
BseRI 707 GAGGAG GAGGAG(10/8)
BsiHKAI 321 GTGCAC G[AT]GC[AT]^C
BsmI 666 GAATGC GAATGC(1/-1)
Bsp1286I 321 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 843 TGTACA T^GTACA
BspLU11I 840 ACATGT A^CATGT
BsrDI 1099 CATTGC GCAATG(2/0)
BsrGI 843 TGTACA T^GTACA
Bsu36I 561 CCTCAGG CC^T[ACGT]AGG
Cac8I 35 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 981 GGGCC G^G[ACGT]CC
DsaI 25 CCGTGG C^C[GA][CT]GG
EarI 567 GAAGAG CTCTTC(1/4)
Eco81I 561 CCTCAGG CC^T[ACGT]AGG
HincII 79 GTCAAC GT[CT]^[GA]AC
HindII 79 GTCAAC GT[CT]^[GA]AC
Ksp632I 567 GAAGAG CTCTTC(1/4)
MaeI 424 CTAG C^TAG
NlaIV 809 GGATCC GG[ACGT]^[ACGT]CC
NspI 840 ACATGT [GA]CATG^[CT]
PflMI 637 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 475 CAGCTG CAG^CTG
Sau96I 981 GGGCC G^G[ACGT]CC
SexAI 86 ACCTGGT A^CC[AT]GGT
SfaNI 712 GATGC GCATC(5/9)
SfcI 791 CTATAG C^T[GA][CT]AG
SmlI 487 CTCAAG C^T[CT][GA]AG
SspBI 843 TGTACA T^GTACA
StuI 741 AGGCCT AGG^CCT
Van91I 637 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 413 GAATTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 156 GCGG CCGC(-3/-1)
915 GCGG
BbvI 477 GCTGC GCAGC(8/12)
480 GCTGC
BsmAI 434 GAGAC GTCTC(1/5)
883 GAGAC
BsrI 354 ACTGG ACTGG(1/-1)
747 CCAGT
BstF5I 101 CATCC GGATG(2/0)
217 GGATG
Fnu4HI 477 GCTGC GC^[ACGT]GC
480 GCTGC
FokI 101 CATCC GGATG(9/13)
217 GGATG
HaeIII 742 GGCC GG^CC
982 GGCC
HinfI 176 GAATC G^A[ACGT]TC
297 GAATC
HphI 45 GGTGA GGTGA(8/7)
294 GGTGA
ItaI 477 GCTGC GC^[ACGT]GC
480 GCTGC
MslI 324 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
946 CATTTTTATG
MspA1I 475 CAGCTG C[AC]G^C[GT]G
913 CAGCGG
TfiI 176 GAATC G^A[AT]TC
297 GAATC
TseI 477 GCTGC G^C[AT]GC
480 GCTGC
Tsp45I 467 GTGAC ^GT[GC]AC
835 GTCAC
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
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AfaI AluI AlwI BpmI BsaJI
BsiYI BslI BssKI BstNI BstYI
DdeI DpnII EcoRII EcoT14I MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII RsaI
Sau3AI ScrFI StyI TaqI Tru9I
Tsp509I TspRI TthHB8I XhoII