Restriction map for
pF1KSDA0201
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AhdI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsiWI BsmBI BspDI BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FauI FseI FspI HgaI
Hin1I HpaI KasI KpnI Ksp632I
KspI MfeI MluI MluNI MscI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccII 45 CGCG CG^CG
AccIII 892 TCCGGA T^CCGGA
AgeI 95 ACCGGT A^CCGGT
Alw44I 99 GTGCAC G^TGCAC
ApaLI 99 GTGCAC G^TGCAC
AseI 2082 ATTAAT AT^TAAT
AsnI 2082 ATTAAT AT^TAAT
Asp700I 1240 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 53 CCCGGG C^[CT]CG[GA]G
BamHI 2328 GGATCC G^GATCC
BclI 2322 TGATCA T^GATCA
BsaAI 2339 TACGTG [CT]AC^GT[GA]
BsaBI 2323 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 892 TCCGGA T^CCGGA
BsiEI 93 CGACCG CG[GA][CT]^CG
BsoBI 53 CCCGGG C^[CT]CG[GA]G
Bsp1407I 311 TGTACA T^GTACA
BspEI 892 TCCGGA T^CCGGA
BsrFI 95 ACCGGT [GA]^CCGG[CT]
BsrGI 311 TGTACA T^GTACA
BstUI 45 CGCG CG^CG
Cac8I 26 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 1028 GCGC GCG^C
Cfr10I 95 ACCGGT [GA]^CCGG[CT]
DraII 1311 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 1458 CCATGG C^C[GA][CT]GG
EcoNI 685 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1311 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1037 GAATTC G^AATTC
FbaI 2322 TGATCA T^GATCA
HaeII 1027 GGCGCT [GA]GCGC^[CT]
HhaI 1028 GCGC GCG^C
HinP1I 1028 GCGC G^CGC
HindIII 1328 AAGCTT A^AGCTT
MamI 2323 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 892 TCCGGA T^CCGGA
MvnI 45 CGCG CG^CG
NcoI 1458 CCATGG C^CATGG
PinAI 95 ACCGGT A^CCGGT
PshBI 2082 ATTAAT AT^TAAT
PstI 2167 CTGCAG CTGCA^G
SacI 831 GAGCTC GAGCT^C
SfcI 2167 CTGCAG C^T[GA][CT]AG
SmaI 53 CCCGGG CCC^GGG
SspBI 311 TGTACA T^GTACA
SspI 2458 AATATT AAT^ATT
XbaI 1426 TCTAGA T^CTAGA
XcmI 1760 CCAAAATAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 53 CCCGGG C^CCGGG
XmaI 53 CCCGGG C^CCGGG
XmnI 1240 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 1380 CCGC CCGC(-3/-1)
46 GCGG
AflIII 1835 ACATGT A^C[GA][CT]GT
2383 ACATGT
AvaII 1951 GGACC G^G[AT]CC
2043 GGACC
BanII 24 GGGCTC G[GA]GC[CT]^C
831 GAGCTC
BpmI 1742 CTCCAG CTGGAG(16/14)
2099 CTCCAG
BsaI 70 GAGACC GGTCTC(1/5)
1557 GAGACC
BsaWI 95 ACCGGT [AT]^CCGG[AT]
892 TCCGGA
BseRI 2002 CTCCTC GAGGAG(10/8)
1510 GAGGAG
BspHI 1248 TCATGA T^CATGA
2294 TCATGA
BspLU11I 1835 ACATGT A^CATGT
2383 ACATGT
BstNI 570 CCTGG CC^[AT]GG
1626 CCAGG
BstYI 558 GGATCT [GA]^GATC[CT]
2328 GGATCC
DraI 742 TTTAAA TTT^AAA
1159 TTTAAA
EaeI 213 TGGCCG [CT]^GGCC[GA]
2134 CGGCCA
EcoRII 570 CCTGG ^CC[AT]GG
1626 CCAGG
EcoT14I 1347 CCAAGG C^C[AT][AT]GG
1458 CCATGG
MflI 558 GGATCT [GA]^GATC[CT]
2328 GGATCC
MvaI 570 CCTGG CC^[AT]GG
1626 CCAGG
NdeI 1955 CATATG CA^TATG
2060 CATATG
NspI 1835 ACATGT [GA]CATG^[CT]
2383 ACATGT
RcaI 1248 TCATGA T^CATGA
2294 TCATGA
SmlI 258 CTTGAG C^T[CT][GA]AG
397 CTCAAG
StyI 1347 CCAAGG C^C[AT][AT]GG
1458 CCATGG
Tsp45I 909 GTCAC ^GT[GC]AC
1180 GTCAC
XhoII 558 GGATCT [GA]^GATC[CT]
2328 GGATCC
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AspHI BbsI BbvI BcnI
BfaI BmyI BpuAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
Cfr13I DdeI DpnII Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I