Restriction map for
pF1KSDA0193
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BanII BbeI BbsI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tth111I Van91I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 603 TCCGGA T^CCGGA
AcsI 78 AAATTC [GA]^AATT[CT]
Alw44I 159 GTGCAC G^TGCAC
ApaLI 159 GTGCAC G^TGCAC
ApoI 78 AAATTC [GA]^AATT[CT]
AvaI 502 CTCGAG C^[CT]CG[GA]G
BbvCI 1101 GCTGAGG CCTCAGC(-5/-2)
BclI 132 TGATCA T^GATCA
BcnI 86 CCCGG CC^[GC]GG
BfaI 697 CTAG C^TAG
BsaI 505 GAGACC GGTCTC(1/5)
BseAI 603 TCCGGA T^CCGGA
BsgI 751 GTGCAG GTGCAG(16/14)
BsmI 869 GCATTC GAATGC(1/-1)
BsoBI 502 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1040 TGTACA T^GTACA
BspEI 603 TCCGGA T^CCGGA
BsrFI 1028 GCCGGC [GA]^CCGG[CT]
BsrGI 1040 TGTACA T^GTACA
BssSI 1051 CACGAG CACGAG(-5/-1)
BstXI 581 CCACTAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 330 GGATCT [GA]^GATC[CT]
Cfr10I 1028 GCCGGC [GA]^CCGG[CT]
DsaI 1145 CCATGG C^C[GA][CT]GG
FauI 217 CCCGC CCCGC(4/6)
FbaI 132 TGATCA T^GATCA
HincII 1216 GTTGAC GT[CT]^[GA]AC
HindII 1216 GTTGAC GT[CT]^[GA]AC
HinfI 802 GACTC G^A[ACGT]TC
MaeI 697 CTAG C^TAG
MflI 330 GGATCT [GA]^GATC[CT]
MroI 603 TCCGGA T^CCGGA
NaeI 1028 GCCGGC GCC^GGC
NciI 86 CCCGG CC^[GC]GG
NcoI 1145 CCATGG C^CATGG
NgoMIV 1028 GCCGGC G^CCGGC
PaeR7I 502 CTCGAG C^TCGAG
PleI 802 GACTC GAGTC(4/5)
PstI 5 CTGCAG CTGCA^G
PvuII 299 CAGCTG CAG^CTG
ScaI 518 AGTACT AGT^ACT
SfcI 5 CTGCAG C^T[GA][CT]AG
SmlI 502 CTCGAG C^T[CT][GA]AG
SspBI 1040 TGTACA T^GTACA
StuI 1134 AGGCCT AGG^CCT
XbaI 696 TCTAGA T^CTAGA
XcmI 1112 CCATGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 502 CTCGAG C^TCGAG
XhoII 330 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 519 GTAC GT^AC
1041 GTAC
BbrPI 44 CACGTG CAC^GTG
1061 CACGTG
BsaAI 44 CACGTG [CT]AC^GT[GA]
1061 CACGTG
BsaWI 141 ACCGGA [AT]^CCGG[AT]
603 TCCGGA
BseRI 11 CTCCTC GAGGAG(10/8)
1104 GAGGAG
DraII 190 AGGACCT [GA]G^G[ACGT]CC[CT]
1196 GGGACCT
EcoO109I 190 AGGACCT [GA]G^G[ACGT]CC[CT]
1196 GGGACCT
HaeIII 89 GGCC GG^CC
1135 GGCC
HphI 578 TCACC GGTGA(8/7)
818 TCACC
MseI 1229 TTAA T^TAA
1238 TTAA
MslI 582 CACTAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
744 CATCACAGTG
MspA1I 299 CAGCTG C[AC]G^C[GT]G
786 CAGCGG
PmaCI 44 CACGTG CAC^GTG
1061 CACGTG
PmlI 44 CACGTG CAC^GTG
1061 CACGTG
PpuMI 190 AGGACCT [GA]G^G[AT]CC[CT]
1196 GGGACCT
RsaI 519 GTAC GT^AC
1041 GTAC
Tru9I 1229 TTAA T^TAA
1238 TTAA
Tsp45I 538 GTCAC ^GT[GC]AC
1079 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HapII HpaII ItaI
MaeII MaeIII MboI MboII MnlI
MspI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp509I TspRI
TthHB8I